A kind of extraction process of vasopressin solution

An extraction process, the technology of vasopressin, applied in the field of bioengineering, achieves the effects of short production cycle, low production cost and simple operation

Active Publication Date: 2021-01-15
NANJING XINBAI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of resin exchange, ultrafiltration, preparative high-performance liquid phase purification and other technologies is used to purify and separate to solve this problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 1000g of the posterior pituitary powder, add 20L of 0.2% acetic acid solution to the pituitary powder for the first time, stir at 45°C for 10 minutes, filter to obtain the filtrate and filter cake, and then add 0.2% acetic acid solution to the filter cake 10 L, stirred at 45°C for 10 min to obtain a filtrate, and the two filtrates were combined. The filtrate was stirred and heated to 95 ° C, kept for 10 min and then cooled to room temperature. Equilibrate the weakly acidic ion exchange resin column with a balance solution with a pH of 3.5, elute through the balanced ion column with a sodium chloride solution with a concentration of 0.1M, collect the eluate, and carry out ultraviolet detection at 280nm on the eluate. When the original absorbance value rises, collect vasopressin. While collecting vasopressin, constantly use acid to adjust the pH value of the collection solution to about 3.5. When the absorbance value drops to 0.4, stop collecting. Pass the collecte...

Embodiment 2

[0027] Weigh 800g of the posterior pituitary gland powder, add 20L of 0.25% acetic acid solution to the pituitary gland posterior leaf powder for the first time, stir at 50°C for 15 minutes, filter to obtain the filtrate and filter cake, and then add 0.25% acetic acid solution to the filter cake 12 L, stirred at 50°C for 15 min to obtain a filtrate, and the two filtrates were combined. The filtrate was stirred and heated to 98°C, kept for 15 minutes and then cooled to room temperature. Equilibrate the weakly acidic ion exchange resin column with a balance solution with a pH of 4.0, elute through the balanced ion column with a sodium chloride solution with a concentration of 0.2M, collect the eluate, and carry out ultraviolet detection at 280nm on the eluate. When the original absorbance value rises, collect vasopressin. While collecting vasopressin, constantly use acid to adjust the pH value of the collection solution to about 3.8. When the absorbance value drops to 0.4, stop ...

Embodiment 3

[0029]Weigh 600g of the posterior pituitary powder, add 18L of 0.3% acetic acid solution to the pituitary powder for the first time, stir at 55°C for 20min, filter to obtain the filtrate and filter cake, and then add 0.3% acetic acid solution to the filter cake 12 L, stirred at 55°C for 20 min to obtain a filtrate, and the two filtrates were combined. The filtrate was stirred and heated to 100°C, kept for 20 minutes and then cooled to room temperature. Equilibrate the weakly acidic ion exchange resin column with a balance solution with a pH of 4.5, elute through the balanced ion column with a sodium chloride solution with a concentration of 0.3M, collect the eluate, and carry out ultraviolet detection at 280nm on the eluate. When the original absorbance value rises, collect vasopressin. While collecting vasopressin, keep using acid to adjust the pH value of the collection solution to about 4.0. When the absorbance value drops to 0.4, stop collecting. Pass the collected vasopr...

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PUM

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Abstract

The invention discloses extraction process of a vasopressin solution. The extraction process includes the steps of raw material extraction, protein removal, resin exchange, ultrafiltration, and purification through preparative high performance liquid chromatography. The process combines various separation and purification methods to directly separate and purify the vasopressin from pituitary of animals. The raw material is low in cost and is easy to obtain. The product is high in yield and purity. The process has simple operations, is environment-friendly, is short in production period and is low in production cost, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to a biological extraction method of vasopressin, a medicinal raw material, belonging to the field of bioengineering, in particular to an extraction process of vasopressin solution. Background technique [0002] Vasopressin, also known as vasopressin and antidiuretic hormone, is a 9-peptide hormone secreted by the nerve cells of the supraoptic nucleus and paraventricular nucleus of the hypothalamus. The molecular formula is: C43H67N15O12S2, and the molecular weight is: 1050.22. Released posteriorly by the posterior lobe of the neurohypophysis. Its main function is to improve the permeability of the distal convoluted tubule and collecting duct to water, promote water absorption, and is a key regulatory hormone for urine concentration and dilution. It is mainly used for the treatment of diabetes insipidus and esophageal variceal bleeding. [0003] There is no report on the preparation of vasopressin using biological extraction met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/16C07K1/36C07K1/18C07K1/34C07K1/20
CPCC07K7/16Y02P20/582
Inventor 沈飞李栋芸于辉冯旌朱小龙潘爱梅高旭江夏芸
Owner NANJING XINBAI PHARMA
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