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Airlift micro-algae biofilm cultivation system

A microalgae biology and culture system technology, applied in enzymology/microbiology devices, methods of supporting/immobilizing microorganisms, biomass post-processing, etc., can solve the problem of low accuracy, achieve good transfer temperature, and improve accuracy , Improve the effect of cultivating safety

Active Publication Date: 2017-05-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing technologies such as authorized announcement number CN 103773673 B A cylindrical air-lift high-efficiency photobioreactor for microalgae cultivation and its application This invention discloses a cylindrical air-lift high-efficiency photobioreactor for microalgae cultivation The reactor and its application include a light-transmitting culture tank, a light control device arranged outside the light-transmitting culture tank, a temperature control device arranged inside the light-transmitting culture tank, and a gas supply device arranged at the bottom of the light-transmitting culture tank. The top of the light culture tank is provided with an inoculation sampling port and an air outlet, and a second filter is provided on the air outlet. The bottom of the light-transmitting tank is provided with an algae liquid collection port, and a valve is provided on the algae liquid collection port. The reactor adopts a height A cylindrical tank with a large diameter ratio, and a lighting device with replaceable and adjustable light intensity and light quality is installed to solve the self-shading phenomenon of algae cells after the increase of microalgae culture density and the demand for different light quality of specific algae species. , to ensure sufficient light energy supply, improve the photosynthetic efficiency of microalgae, and easily achieve high-density cultivation, but it seems that only one temperature monitoring device is used to detect the temperature in the cultivation tank. The accuracy is not high, and the safety of light control is still Room for improvement

Method used

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  • Airlift micro-algae biofilm cultivation system
  • Airlift micro-algae biofilm cultivation system
  • Airlift micro-algae biofilm cultivation system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Such as Figure 1~4 Shown: an air-lift microalgae biofilm cultivation system, including a cultivation tank 1, a shell 2, a temperature controller 3, and a gas cylinder 4. The bottom of the cultivation tank 1 is provided with a temperature control rod 33, an air stone 42, and a collection port 6 , the culture tank 1 is connected to the collection port 6 through a conduit, the collection port 6 is provided with a valve 5, and the gas stone 42 is evenly distributed on the bottom of the culture tank 1, and the gas stone 42 is connected to the gas supply port 43 through the conduit, and the gas supply port 43 is connected to the gas supply port 43 through the conduit. The first filter 41 is connected, the first filter 41 is connected with the first flow meter 44 and the second flow meter 46 through a tee joint, the first flow meter 44 is connected with the air pump 45, and the second flow meter 46 is connected with the gas cylinder 4 connection, the air pump 45 and the gas c...

Embodiment 2

[0023] Such as Figure 1~4As shown, the bottom of the culture tank 1 is provided with a temperature control rod 33, an air stone 42, and a collection port 6. The culture tank 1 is connected to the collection port 6 through a conduit, and the collection port 6 is provided with a valve 5. The air stones 42 are evenly distributed in the culture tank 1 At the bottom, the gas stone 42 is connected to the gas supply port 43 through a conduit, the gas supply port 43 is connected to the first filter 41 through a conduit, and the first filter 41 is connected to the first flowmeter 44 and the second flowmeter 46 through a three-way joint , the first flowmeter 44 is connected with the air pump 45, the second flowmeter 46 is connected with the gas cylinder 4, the air pump 45 and the gas cylinder 4 are respectively connected with an air filter, and the top of the culture tank 1 is provided with an air outlet 7 and a sample inlet 11, the air outlet 7 is provided with a second filter 8, the ...

Embodiment 3

[0028] Such as Figure 1~4 As shown, the culture tank 1 is sterilized before inoculation, the temperature probe 31 and the optical fiber are taken out of the culture tank 1 during sterilization, and the culture medium is added to the culture tank 1 and sterilized by high-pressure steam, and the culture medium is the culture tank 1 80% of the volume, light-emitting diode groups 21 are installed on the inner wall of the housing 2, and the light quality is white light. The combination of diode group 21 can provide 4 levels of light intensity ranging from 30-120 μmol photons m-2s-1, and each level increases in turn by 30 μmol photons m-2s-1. The components are installed to form an airlift microalgae biofilm culture system, and then the microalgae cells are inoculated into the culture tank 1 through the sampling port 11 at an inoculation density of 0.4×107 cells / mL, and cultured under continuous light.

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Abstract

The invention provides an airlift micro-algae biofilm cultivation system which comprises a cultivation tank, a shell, a temperature controller and a gas cylinder. A temperature control rod and gas relay rock are arranged at the bottom of the cultivation tank, a collecting opening is formed in the bottom of the cultivation tank, a gas outlet and a sample injecting opening are formed in the top of the cultivation tank, the cultivation tank is provided with an optical fiber tube and a jacket, a temperature probe is arranged in the jacket and is connected with the temperature controller, the temperature controller is connected with the temperature control rod, optical fibers are arranged in the optical fiber tube and are connected with an industrial personal computer, a central control room and an alarm are connected with the industrial personal computer, the cultivation tank is a transmittance cultivation tank, and a light emitting diode group is arranged on the inner wall of the shell and is connected with an illumination controller. The airlift micro-algae biofilm cultivation system has the advantages that the airlift micro-algae biofilm cultivation system is provided with double temperature detection devices and alarm devices, the cultivation tank is good in temperature resistance, the light intensity and the light quality can be effectively controlled, the micro-algae cultivation yield can be increased, and the micro-algae cultivation quality can be improved.

Description

technical field [0001] The invention belongs to the technical field of biological cultivation, and in particular relates to an airlift microalgae biofilm cultivation system. Background technique [0002] Microalgae cells are rich in amino acids, proteins, vitamins, unsaturated fatty acids and other high value-added biological substances, and have become an important source of materials in the fields of human food, medicine, dyes, and fine chemicals. At present, with the depletion of fossil energy such as petroleum and coal, people have paid great attention to biorefinery based on biomass. As an important renewable energy source, microalgae can provide a large amount of biomass (fat, starch, cellulose) , has broad application prospects in the field of biorefinery. [0003] Existing technologies such as authorized announcement number CN 103773673 B A cylindrical air-lift high-efficiency photobioreactor for microalgae cultivation and its application This invention discloses a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/12C12M1/00
CPCC12M21/02C12M23/20C12M23/22C12M37/02C12M41/06C12M41/12
Inventor 高锋李晨崔伟彭苑媛
Owner ZHEJIANG OCEAN UNIV