An airlift microalgae biofilm cultivation system
A microalgae biology and culture system technology, applied in enzymology/microbiology devices, methods of supporting/immobilizing microorganisms, biomass post-processing, etc., can solve the problem of low accuracy, achieve good transfer temperature, and improve accuracy , Improve the effect of cultivating safety
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Embodiment 1
[0018] like Figure 1~4 Shown: an air-lift microalgae biofilm cultivation system, including a cultivation tank 1, a shell 2, a temperature controller 3, and a gas cylinder 4. The bottom of the cultivation tank 1 is provided with a temperature control rod 33, an air stone 42, and a collection port 6 , the culture tank 1 is connected to the collection port 6 through a conduit, the collection port 6 is provided with a valve 5, and the gas stone 42 is evenly distributed on the bottom of the culture tank 1, and the gas stone 42 is connected to the gas supply port 43 through the conduit, and the gas supply port 43 is connected to the gas supply port 43 through the conduit. The first filter 41 is connected, the first filter 41 is connected with the first flow meter 44 and the second flow meter 46 through a tee joint, the first flow meter 44 is connected with the air pump 45, and the second flow meter 46 is connected with the gas cylinder 4 connection, the air pump 45 and the gas cyli...
Embodiment 2
[0023] like Figure 1~4As shown, the bottom of the culture tank 1 is provided with a temperature control rod 33, an air stone 42, and a collection port 6. The culture tank 1 is connected to the collection port 6 through a conduit, and the collection port 6 is provided with a valve 5. The air stones 42 are evenly distributed in the culture tank 1 At the bottom, the gas stone 42 is connected to the gas supply port 43 through a conduit, the gas supply port 43 is connected to the first filter 41 through a conduit, and the first filter 41 is connected to the first flowmeter 44 and the second flowmeter 46 through a three-way joint , the first flowmeter 44 is connected with the air pump 45, the second flowmeter 46 is connected with the gas cylinder 4, the air pump 45 and the gas cylinder 4 are respectively connected with an air filter, and the top of the culture tank 1 is provided with an air outlet 7 and a sample inlet 11, the air outlet 7 is provided with a second filter 8, the cul...
Embodiment 3
[0028] like Figure 1~4 As shown, the culture tank 1 is sterilized before inoculation, the temperature probe 31 and the optical fiber are taken out of the culture tank 1 during sterilization, and the culture medium is added to the culture tank 1 and sterilized by high-pressure steam, and the culture medium is the culture tank 1 80% of the volume, light-emitting diode groups 21 are installed on the inner wall of the housing 2, and the light quality is white light. The combination of diode group 21 can provide 4 levels of light intensity ranging from 30-120 μmol photons m-2s-1, and each level increases in turn by 30 μmol photons m-2s-1. The components are installed to form an airlift microalgae biofilm culture system, and then the microalgae cells are inoculated into the culture tank 1 through the sampling port 11 at an inoculation density of 0.4×107 cells / mL, and cultured under continuous light.
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