Excited Raman spectrum imaging system based on fast scanning optical path

Abstract

The invention belongs to the technical field of non-linear optical imaging and particularly relates to an excited Raman spectrum imaging system based on a fast scanning optical path. A galvanometer scanning unit capable of quickly generating optical path delaying and the excited Raman spectrum imaging system are combined together, and the excited Raman spectrum imaging system based on the fast scanning optical path is simple in structure, low in modification cost and easy to implement. Since the excited Raman spectrum imaging system can quickly generate the optical path delaying required by a spectral focusing method, excited Raman spectrums of a sample can be quickly collected, the spectrums collected many times can be averaged, so that the signal-to-noise ratio can be greatly improved, and thus the detection sensitivity is improved; target objects of various ingredients are detected in real time; and when Raman spectrums of two target objects in a measured sample overlap (such as lipid and protein), spectral errors caused by movement of the sample are eliminated. According to the system, the excited Raman spectrums can be quickly collected, and simultaneous imaging can be performed according to multiple Raman vibrational peaks.

Description

technical field [0001] The invention belongs to the technical field of nonlinear optical imaging, and in particular relates to an improved stimulated Raman spectrum imaging system, which can realize fast stimulated Raman spectrum acquisition and simultaneously image multiple Raman vibration peaks at the same time system. Background technique [0002] Stimulated Raman imaging technology is a new non-linear optical imaging method. With many advantages such as high signal intensity, no spectral distortion, and linear relationship between signal intensity and analyte concentration, stimulated Raman imaging has become more and more popular in recent years. Technology has developed rapidly and is widely used in biomedical detection and imaging, biochemical analysis and other fields. [0003] Stimulated Raman scattering is an organic combination of Raman scattering and stimulated radiation technology, which requires two beams of high-energy-density pulsed lasers with different fre...

AI Technical Summary

Problems solved by technology

However, the conventional method of adjusting the position of the optical delay line is to move the position of the mirror by means of mechanical translation, which is slow and cannot detect two or more Raman vibration frequencies in real time.

For fast-moving objects that require two-color or multi-color imaging, multiple monochrome images for different Raman peaks will inevitably introduce errors when they are combined into one multi-color image

Moreover, the acquisition of stimulated Raman spectra is also limited by the moving speed of the optical delay line, which cannot achieve the effect of fast measurement of spectra

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