Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for synthesizing manganese dioxide nanowire by using filamentous phage template and application of manganese dioxide nanowire

A technology of manganese dioxide nanometer and filamentous bacteriophage, which is applied in the fields of catalysis, analytical chemistry and nanomaterials. It can solve the problems of mild preparation that have not been reported yet, and achieve good application prospects, uniform appearance and simple synthesis method.

Inactive Publication Date: 2017-05-31
QINGDAO UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, to date, MnO based on filovirus templates or other biological templates 2 Gentle preparation of nanowires has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for synthesizing manganese dioxide nanowire by using filamentous phage template and application of manganese dioxide nanowire
  • Method for synthesizing manganese dioxide nanowire by using filamentous phage template and application of manganese dioxide nanowire
  • Method for synthesizing manganese dioxide nanowire by using filamentous phage template and application of manganese dioxide nanowire

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of M13 phage:

[0038] Take 1 μL of phagemid PC89 solution, transform it into Escherichia coli TG1 competent cells, inoculate a single colony in 2 mL of Amp-resistant SOC culture medium after plate culture, culture with shaking at 37 °C for 2 h, transfer to 400 mL of Amp In the resistant SOC culture solution, shake culture at 37 °C until the absorbance of the culture solution at 600 nm is about 0.4, add about 1×10 12 A helper phage M13KO7 was cultured with shaking for 1 h, then kanamycin with a final concentration of 25 µg / mL and isopropyl-β-d-thiogalactoside with a final concentration of 1 mM were added, and cultured with shaking at 37 °C overnight.

Embodiment 2

[0040] Purification of M13 phage:

[0041] Place the culture solution in a 70 °C water bath for 20 min, then divide into 50 mL centrifuge tubes, centrifuge for 15 min (rotating speed 5000rpm), collect the supernatant; add PEG8000 to 40 g / L, NaCl to 30 g / L, and store on ice For 1 h, centrifuge at 4°C for 5 min (12,000 rpm), discard the supernatant; dissolve the pellet with 5 mL of PBS, centrifuge at 4°C for 5 min (12,000 rpm), and discard the insoluble matter. Repeat the above method once, collect the supernatant, and store at 4°C. The concentration of M13 phage in the supernatant was determined by the common method of plate counting phage plaques.

Embodiment 3

[0043] Preparation of manganese dioxide nanowires based on filamentous bacteriophage M13 template:

[0044] (1) Mix the filamentous bacteriophage and the aqueous solution of manganese acetate evenly, and let it stand for 1 h;

[0045] (2) Add an aqueous solution of NaOH to the above-mentioned mixed solution and mix well, and generate a yellow-brown suspension after shaking for 24 h;

[0046] (3) Centrifuge the above reaction solution, remove the supernatant containing unreacted components, collect the precipitate, and store it at 4°C for future use; the purified manganese dioxide nanowires can also be collected by dialysis or filtration.

[0047] Another control experiment was done: manganese dioxide was synthesized in the absence of M13 phage, and other experimental conditions and steps were the same as the above experiment.

[0048] Such as figure 1 As shown, the mixed reaction solution of M13 bacteriophage and manganese acetate produced a large amount of precipitation aft...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to the fields of nanomaterials, catalysis and analytical chemistry, in particular to a preparation method for synthesizing a manganese dioxide nanowire on the basis of using filamentous phage as a biological template and the application of the manganese dioxide nanowire in catalysis aspect. The method comprises the following steps: mixing the filamentous phage with manganous ions by using inherent nanostructure characteristics of the filamentous phage and properties of capsid protein of the filamentous phage; adding sodium hydroxide and carrying out normal temperature reaction to generate manganese dioxide on the surface of the filamentous phage, namely obtaining the manganese dioxide nanowire with the diameter of 10 to 50nm. The material has the biomimetic catalytic characteristic and electrochemical catalysis characteristic, so that the material has a broad application prospect in the fields of analytical chemistry, capacitors and catalysis. According to the method, a natural nanobiological structure is used as a template; the method has the advantages of simple preparation process, mild and environment-friendly reaction conditions and easily-controlled nanostructure.

Description

technical field [0001] The invention relates to the fields of nanomaterials, catalysis and analytical chemistry, and specifically includes a preparation method for synthesizing manganese dioxide nanowires based on filamentous bacteriophage as a biological template and its application in catalysis, capacitors, color analysis, and electrochemical sensing . Background technique [0002] Nanomaterials refer to materials with at least one dimension in the nanoscale range (1−100 nm) in three-dimensional space. Nanowire refers to a one-dimensional structure nanomaterial that is confined to less than 100 nanometers in the lateral direction (no restriction in the longitudinal direction). [0003] In recent years, manganese dioxide, as a metal oxide with important industrial uses, has attracted widespread attention in various fields. Researchers have used a variety of methods to synthesize nano-manganese dioxide with different shapes. With its unique structure and properties, it has...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C01G45/02B82Y40/00G01N21/31G01N27/30H01M4/90B01J23/34B01J35/06
CPCH01M4/9016G01N21/31G01N27/308C01G45/02B01J23/34C01P2004/16C01P2002/80C01P2002/01C01P2006/90C01P2006/40B01J35/58B01J35/40Y02E60/50
Inventor 刘爱骅韩磊
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com