Highly oxidized new compound, preparation method and medicinal uses thereof

A compound and composition technology, applied in the field of highly oxidized new compounds and their preparation, can solve the problems of separation and identification of monomers of flavonoids that have not been seen

Inactive Publication Date: 2017-05-31
温州高新技术产业开发区聚智汇科技信息咨询服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The reported components of cardamom are mainly volatile oils, polysaccharides and proteins, and it is speculated that they also contain flavonoids, but so far no flavonoids have been isolated and identified

Method used

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  • Highly oxidized new compound, preparation method and medicinal uses thereof
  • Highly oxidized new compound, preparation method and medicinal uses thereof
  • Highly oxidized new compound, preparation method and medicinal uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0024] Sources of medicinal materials and reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane are analytically pure, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, analytically pure, are purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd. The dried and mature fruits of cardamom were purchased from the Bozhou Traditional Chinese Medicine Market in Anhui Province, and the place of origin is Fujian.

[0025] Preparation method: (a) The dried and mature fruit (10kg) of cardamom is crushed to the size of mung bean, extracted with 80% ethanol under heat reflux (25L×3 times), the extracts are combined, concentrated until no alcohol smell (6L), and sequentially washed with petroleum Ether (6L×3 times), ethyl acetate (6L×3 times) and water-saturated n-butanol (6L×3 times) were extracted and concentrated to obtain petroleum ether extract, ethyl ac...

Embodiment 2

[0027] Embodiment 2: compound (I) pharmacological action test

[0028] 1. Materials and Instruments

[0029] Human gastric cancer cell line SGC-7901 was purchased from Purcell Reagent Company; compound (I) was self-made, and the normalized purity was greater than 98%; CTX (cyclophosphamide, positive drug), trypsin, MTT, dimethyl sulfoxide ( DMSO), agarose, and glacial acetic acid were purchased from Sigma Company in the United States; RPMI-1640 medium and PBS phosphate buffer solution were purchased from GIBCO Company in the United States; fetal bovine serum was purchased from Shandong Yinxiang Weiye Company; trypan blue was purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.; TUNEL kit was purchased from KGI Biotechnology Development Co., Ltd.; DAB chromogenic kit was purchased from Beijing Zhongshan Jinqiao Biological Company; formaldehyde was purchased from Fisher Company of the United States; catalase was purchased from Tianjin Tianxin Fine Chemicals open you...

Embodiment 3

[0062] Preparation of tablets: firstly prepare compound (I) according to the method of Example 1, and utilize organic acids such as tartaric acid, or citric acid, formic acid or oxalic acid, etc., inorganic acids such as hydrochloric acid or sulfuric acid or phosphoric acid to make salts, according to its The weight ratio of the excipient to the excipient is 1:9, and the excipient is granulated and compressed into tablets.

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Abstract

The present invention discloses a highly oxidized new compound, a preparation method and medicinal uses thereof, belongs to the field of medicine, and particularly relates to a new compound separated from the dried mature fruit of eletteria cardamomum and having an epoxy structure, a preparation method and medicinal uses thereof. According to the present invention, the compound is firstly reported, can be extracted, separated and purified from the dried mature fruit of eletteria cardamomum, and has high purity; and in vitro test results prove that the compound can inhibit the proliferation of gastric cancer cells, wherein the compound can separately provide the inhibition activity and can further enhance the inhibition activity by combining with other anti-cancer drugs, such that the compound can be developed and prepared into the gastric cancer treatment drug.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a highly oxidized new compound isolated from the dry ripe fruit of cardamom, a preparation method and a medical application thereof. Background technique [0002] Cardamom is a perennial herb. Rhizome stout, brownish red. The leaves are in two rows, the blades are narrow and long-lanceolate, and the leaf sheaths are brownish-yellow pilose. Spikes emerge from the base of the stem. The inflorescence is markedly elongated, the flowers are sparsely arranged, and the corolla is white. The fruit is oblong, with tough skin and not easy to crack. The seed mass is divided into 3 petals, with 5 to 9 seeds in each petal, and the smell of the seeds is fragrant and strong. Mainly produced in Vietnam, Sri Lanka and the Malabar (Malabar) coast of southern India. Harvest when the fruit is ripe, remove the residual fruit stalk, and dry in the sun. Use the dried fruit of cardamom, a pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/22A61K31/365A61P35/00
CPCC07D493/22
Inventor 张声芬
Owner 温州高新技术产业开发区聚智汇科技信息咨询服务中心
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