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A kind of nitrous acid bacteria enrichment culture method

A technology of nitrous bacteria and enrichment culture, applied in the field of enrichment culture of nitrous bacteria, can solve the problems of difficult and stable control of DO concentration, long culture time, etc. Effect

Active Publication Date: 2020-09-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical engineering applications, it is difficult to stably control the DO concentration within the required low concentration range. Once the DO concentration is high, the short-cut nitrification will transform into the full-range nitrification
[0004] CN1785843A discloses a method for short-range nitrification of wastewater with low C / N ratio and high concentration of ammonia nitrogen. The method uses the nitrification granular sludge cultivated by anaerobic granular sludge as an inoculum, and gradually increases the concentration of ammonia nitrogen in the substrate to realize it. Although oxygen granular sludge has the advantage of fast cultivation speed, in the subsequent aerobic short-cut nitrification process, there are still shortcomings in the transformation from short-cut nitrification to full-range nitrification.
However, this method relies solely on external conditions for enrichment culture, and the culture time is relatively long

Method used

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  • A kind of nitrous acid bacteria enrichment culture method
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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] According to the inoculum amount of 20% (sludge 30-minute sedimentation ratio), the activated sludge rich in nitrifying bacteria of a sewage treatment plant is inserted into the aeration reactor, and the method of gradually increasing the concentration of ammonia nitrogen in the substrate is adopted for cultivation. The initial ammonia nitrogen concentration 50mg / L, increase 50mg / L each time, when the concentration of ammonia nitrogen increases to 300mg / L, add nitrous bacteria growth accelerator I every day according to the accelerator concentration in the culture system of 30mg / L, and add the accelerator at the same time The pH is controlled at 7.0-8.0, and the DO concentration is controlled at 2.0-2.5mg / L. After 8 hours of cultivation, the pH needs to be adjusted to 8.0-9.0, and the DO concentration is reduced to 1.0-1.5mg / L; when the substrate ammonia nitrogen concentration is increased to 500mg / L L and within 24 hours, the ammonia nitrogen removal rate reached 85%, a...

Embodiment 2

[0028] According to the inoculum amount of 30% (sludge 30-minute sedimentation ratio), the activated sludge rich in nitrifying bacteria of a sewage treatment plant is inserted into the aeration reactor, and the method of gradually increasing the concentration of ammonia nitrogen in the substrate is adopted for cultivation. The initial concentration of ammonia nitrogen 80mg / L, increase 50mg / L each time, when the concentration of ammonia nitrogen increases to 300mg / L, add nitrous bacteria growth promoter II every other day according to the concentration of the accelerator in the culture system 25mg / L, after supplementing to promote At the same time, the pH is controlled at 7.0-8.0, and the DO concentration is controlled at 2.5-3.0mg / L. After 24 hours of cultivation, the pH needs to be adjusted to 8.0-9.0, and the DO concentration is reduced to 1.0-1.5mg / L; when the substrate ammonia nitrogen When the concentration was increased to 500mg / L and the ammonia nitrogen removal rate of ...

Embodiment 3

[0030] According to the inoculum amount of 40% (sludge 30-minute sedimentation ratio), the activated sludge rich in nitrifying bacteria of a sewage treatment plant is inserted into the aeration reactor, and the method of gradually increasing the concentration of ammonia nitrogen in the substrate is adopted for cultivation. The initial ammonia nitrogen concentration When the concentration of ammonia nitrogen increases to 300mg / L, start to add nitrous bacteria growth accelerator III once a day according to the accelerator concentration in the culture system of 20mg / L. At the same time, the pH is controlled at 7.0-8.0, and the DO concentration is 2.5-3.0mg / L. After 15 hours of cultivation, the pH needs to be adjusted to 8.0-9.0, and the DO concentration is reduced to 1.0-1.5mg / L; when the substrate ammonia nitrogen concentration is increased to 500mg / L L and within 24 hours, the ammonia nitrogen removal rate of the matrix reached 85%, and the nitrous acid rate reached more than 80...

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Abstract

The invention relates to a nitrite bacteria enrichment culture method, which comprises the following steps: selecting nitrobacteria-rich activated sludge, culturing by a mode of increasing ammonia concentration of a substrate step by step with the initial ammonia concentration being less than 100 mg / L, and supplementing a nitrite bacteria growth promoter when the ammonia concentration is increased to 300 mg / L; and terminating the enrichment culture process when the ammonia concentration of the substrate is raised to 500 mg / L and ammonium-nitrogen removal rate within 24 h reaches 85% and nitrous acid rate reaches 80% and above. The nitrite bacteria growth promoter contains metal salts, a polyamine substance, organic acid hydroxylamine and Na2SO3; and the metal salts are composed of calcium salt, copper salt, magnesium salt and / or ferrous salt. By adding the nitrite bacteria growth promoter and regulating enrichment culture conditions, nitrite bacteria grow and reproduce rapidly within a short period of time, and culture period is shortened. The cultured nitrite bacteria has high denitrification activity, good stability and strong impact resistance.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a method for enriching and cultivating nitrous bacteria. Background technique [0002] Short-range nitrification and denitrification biological denitrification technology, also known as nitrous acid biological denitrification technology, is to control the nitrification process to NO 2 -N stage, followed by nitrite denitrification. Compared with the traditional biological denitrification technology, the short-cut nitrification-denitrification biological denitrification technology can shorten the hydraulic retention time, save 25% energy consumption and 40% carbon source, and can reduce the amount of residual sludge treatment. Therefore, short-cut nitrification-denitrification biological denitrification technology has become a new research hotspot in the field of sewage biological denitrification. In particular, it is of great practical significance ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 高会杰孙丹凤郭志华赵胜楠
Owner CHINA PETROLEUM & CHEM CORP