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Combination of fluorine-resistant ore-leaching bacteria and its engineering continuous expansion culture method and application

A leaching bacteria, fluorine-resistant technology, applied in the direction of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., can solve the problem of non-continuous production of bacteria, the influence of bacterial activity, and shorten the period of bacterial growth retardation. And other issues

Active Publication Date: 2020-12-08
有研资源环境技术研究院(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although improving the external conditions of bacterial growth can shorten the lag period of bacterial growth to a certain extent, the bacterial activity is still limited.
Moreover, the strains introduced in the biometallurgical process are often not produced continuously, which has a certain impact on the maintenance of bacterial activity.

Method used

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  • Combination of fluorine-resistant ore-leaching bacteria and its engineering continuous expansion culture method and application
  • Combination of fluorine-resistant ore-leaching bacteria and its engineering continuous expansion culture method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0064] Embodiment 1 Fluorine-resistant domestication and expanded cultivation of fluorine-resistant ore-leaching bacteria combination

[0065] 1) Collection:

[0066]Collect ore-leaching bacteria solution from pit water and ores of fluorine-containing mines, inoculate the ore-leaching bacteria solution in 9K medium, account for 20% of the inoculum solution, adjust the pH value to 2.0, and place in an air bath constant temperature shaking incubator, The temperature is 33° C., and the rotation speed of the shaker is 160 rpm. Microscopic counting of bacterial growth to the logarithmic phase, Fe in the culture solution 2+ When the concentration is lower than 0.2g / L, concentrate the leaching bacteria solution, and then inoculate it into a new 9K medium for cultivation. Do this until the growth period of the ore-leaching bacteria is shortened to within 2 days and tends to be stable.

[0067] Place the cultured leaching bacteria solution in an Eppendorf Centrifuge 5804R refrigerat...

Embodiment 2

[0077] Embodiment 2 Industrialized expanded cultivation

[0078] Utilize the continuous expansion culture system provided by the invention to carry out, as figure 1 As shown, add uranium extraction tail liquid in batching tank 1, and add industrial ferrous sulfate 22.10Kg / m 3 to supplement Fe 2+ . Mix evenly through the stirring device 5, adjust the pH to 2.1±0.05, enter the inoculation tank 2 to insert bacteria, insert the total amount of fluorine-resistant leaching bacteria to 10%, and the aeration rate is 2.0m 3 / (m 2 h), the temperature is 33±1°C; the bacteria enter the oxidation tank 3 after the adaptation period, and the aeration rate is 2.0m 3 / (m 2 h), the temperature is 33±1°C, and enters the rapid growth stage; finally enters the finished product tank 4, and performs micro-aeration with an aeration rate of 0.5m 3 / (m 2 h), to prevent bacterial decay. Finally, the acid-resistant pump 13 is used to return and introduce strains. Daily continuous production of f...

Embodiment 3

[0080] Example 3: Bioheap leaching of a certain fluorine-containing uranium ore

[0081] For a uranium ore bioheap leaching, the heap size is 4500t, the fluorine content in the ore is 2.2%, and the uranium grade is 0.2%.

[0082] The fluorine-resistant ore leaching bacteria provided by the invention are used in the biological heap leaching test of the uranium ore after being continuously enlarged and cultivated in the project. The conventional leaching rate of uranium is increased from about 85% to more than 90%, the leaching time is shortened by 30-50 days compared with the prior art, and the consumption of sulfuric acid is reduced from about 6% to about 4%.

[0083] The advantages of the present invention are: a combination of fluoride-resistant ore-leaching bacteria provided by the present invention can quickly oxidize Fe 2+ for Fe 3+ , to provide fresh strains for bioheap leaching, which can significantly improve leaching efficiency and reduce production costs. Use wast...

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Abstract

The invention discloses a fluoride-resistant leaching bacterium composition and an engineering continuous enlarged culture method and application thereof. The fluoride-resistant leaching bacterium composition is formed by a co-culture KF-III of Acidithiobacillus ferrivorans Retech KF-I, Acidithiobacillus ferrooxidans Retech KF-II, Acidithiobacillus ferrooxidans and Leptospirillum ferriphilum Retech. The fluoride-resistant leaching bacterium composition disclosed by the invention can grow under acidic fluoride-containing conditions and is capable of rapidly oxidizing Fe<2+> into Fe<3+>. The fluoride-resistant leaching bacterium composition can be cultured on a large scale by using engineering continuous enlarged culture equipment so as to produce a leaching agent containing a leaching bacterial strain and Fe<3+> and having a double oxidizing effect, and besides, a part of bacteria solution is returned for inoculation. The strain cultured by using the engineering continuous enlarged culture method has the advantages of stable activity and good adaptation and can be widely applied in the field of biological metallurgy.

Description

technical field [0001] The invention relates to the technical field of biometallurgy, in particular to a combination of fluorine-resistant ore leaching mixed bacteria and its engineering continuous expansion cultivation method and application. Background technique [0002] Biometallurgy technology has the characteristics of clean, short process and low cost, and is often used in the extraction of valuable metals such as copper, uranium, molybdenum, vanadium, cobalt, zinc and other metals in ores. At present, there are about 20 kinds of ore leaching bacteria, and most of them belong to chemoautotrophic bacteria. The most commonly used ore leaching bacteria are Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirilum ferriphilum. These bacteria rely on various inorganic salts to survive during the growth and reproduction process, and can obtain the energy required for the life process by oxidizing ferrous iron, elemental sulfur, and reduced compounds, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/36C22B3/18C12M1/38C12M1/36C12R1/01
CPCY02P10/20
Inventor 莫晓兰温建康武彪武名麟
Owner 有研资源环境技术研究院(北京)有限公司
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