Method for inhibiting infection rice SA path relevant gene up-regulation

A technology of rice and pathways, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc.

Inactive Publication Date: 2017-05-31
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most studies focus on how pH affects the interaction mechanism between the host and the pathogen. There are few studies on how pH affects the expression of genes related to the SA pathway in rice infected by pathogenic effector gene overexpression strains.

Method used

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  • Method for inhibiting infection rice SA path relevant gene up-regulation
  • Method for inhibiting infection rice SA path relevant gene up-regulation
  • Method for inhibiting infection rice SA path relevant gene up-regulation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The spore suspension of Magnaporthe oryzae was treated with pH 5, and the spore suspension was inoculated on rice leaves at the three-leaf and one-heart stage, kept in the dark at 28°C for 24 hours, and then placed in a greenhouse for heat preservation and moisture retention. Samples were taken at 0h, 24h, 48h and 96h after inoculation, the total RNA was extracted with Trizol RNA extraction kit, and the expression of two genes (PAL and EDS1) related to the SA pathway was detected with a real-time fluorescence quantitative kit (Table 1).

[0026] Table 1 Expression levels of PAL and EDS1 at pH 5.0 treated with blast fungus overexpressed strains infecting rice at different time points

[0027]

Embodiment 2

[0029] The spore suspension of Magnaporthe grisea was treated with pH 5.5, and the spore suspension was inoculated on rice leaves at the three-leaf-one-heart stage, kept in the dark at 28°C for 24 hours, and then placed in a greenhouse for heat preservation and moisture retention. Samples were taken at 0h, 24h, 48h and 96h after inoculation, the total RNA of the samples was extracted with the Trizol RNA extraction kit, and the expression of two genes (PAL and EDS1) related to the SA pathway was detected with a real-time fluorescent quantitative kit (Table 2).

[0030] Table 2 Expression levels of PAL and EDS1 at pH 5.5 treated with blast fungus overexpressed strains infecting rice at different time points

[0031]

Embodiment 3

[0033] The spore suspension of Magnaporthe grisea was treated with pH 8.0, and the spore suspension was inoculated on rice leaves at the three-leaf-one-heart stage. After 24 hours of dark moisturizing at 28°C, it was placed in a greenhouse for heat preservation and moisturizing. Samples were taken at 0h, 24h, 48h and 96h after inoculation, the total RNA was extracted with the Trizol RNA extraction kit, and the expression of two genes (PAL and EDS1) related to the SA pathway was detected with a real-time fluorescent quantitative kit (Table 3).

[0034] Table 3 Expression levels of PAL and EDS1 at pH 8.0 treated with overexpressed strains of Magnaporthe oryzae infecting rice at different time points

[0035]

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Abstract

The invention discloses a method for inhibiting infection rice SA path relevant gene up-regulation. Weak acid/ alkali pH5, pH5.5, pH8 and pH8.4 solutions are used for treating magnaporthe grisea effect protein gene overexpression strain spores; spore suspension treated by solutions with different pH values are sprayed for inoculating rice leaves; sampling is performed in the time points of 0h, 24h, 48h, 72h and 96h; the rice sample total RNA (ribonucleic acid) is extracted; reverse transcription is performed to obtain cDNA (complementary deoxyribonucleic acid); real-time RT-PCR (reverse transcription-polymerase chain reaction) is performed for detecting cDNA; the expression of two major genes PAL and EDS1 relevant to the SA path in different time points is analyzed. Compared with the prior art, the invention provides the method for more simply, conveniently, directly, effectively and accurately quantifying the influence on the rice SA path relevant gene overexpression after the rice infection by pathogenic bacteria gene overexpression strain subjected to weak acid/alkali treatment; the important experiment basis is provided for the study on the weak acid/alkali response mechanism during the fast and accurately analysis on the rice and magnaporthe grisea interaction.

Description

technical field [0001] The invention belongs to the field of plant protection and biotechnology, and in particular relates to a method for inhibiting the up-regulation of SA pathway-related genes of rice infecting rice by weak acid / alkali treatment of blast fungus effector protein gene overexpressed strains. Background technique [0002] As more and more attention has been paid to the impact of global climate change on agricultural production, many studies have focused on the impact of various climate changes on the prevalence of plant diseases and the specific mechanisms of climate-altered plant pathogen-host interactions. The emergence of plant diseases stems from the interaction among susceptible host plants, pathogenic bacteria, and environmental conditions suitable for disease development, which is known as the disease triangle. Changes in environmental conditions aggravate plant disease symptoms and are associated with 44% of new disease epidemics. Changes in environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/14C12Q1/68C12R1/645
CPCC12N1/14C12N1/36C12Q1/6895C12Q2600/158
Inventor 杨静李成云王春梅刘林吴奇王云锋
Owner YUNNAN AGRICULTURAL UNIVERSITY
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