Method for producing ethanol by fermentation of fresh sugar-containing biomasses

A biomass fermentation and biomass technology, applied in the fields of biomass energy and fermentation engineering, can solve the problems of low concentration, low ethanol yield and high separation cost

Inactive Publication Date: 2017-05-31
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for producing ethanol by fermenting green and fresh sugar-containing biomass in view of the deficiencies in the prior art. Biomass raw materials reduce the cost of production raw materials, and at the same time solve the problems of low ethanol yield, low concentration and high separation cost in the traditional synchronous saccharification ethanol fermentation process

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  • Method for producing ethanol by fermentation of fresh sugar-containing biomasses

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Embodiment approach

[0048] According to some embodiments of the present invention, the method further includes a step of separating and purifying the ethanol-rich fermented mash to obtain an ethanol product.

[0049] In some embodiments of the present invention, the separation and purification method includes one or more of ordinary distillation, vacuum rectification, liquid-liquid extraction, pervaporation, steam permeation, steam stripping, vacuum extraction and adsorption .

[0050] According to the present invention, the separation and purification are carried out after the anaerobic fermentation is terminated, or in-situ separation is carried out coupled with the anaerobic fermentation process.

[0051] In some specific embodiments of the present invention, the method for producing ethanol by fermentation of fresh sugar-containing biomass comprises the following steps:

[0052] (1) Raw material squeezing: squeeze fresh and fresh sugar-containing biomass raw materials to realize solid-liquid...

Embodiment 1

[0069] Example 1: Ethanol was produced by fermenting green and fresh sweet sorghum stalks as raw materials.

[0070] (1) Pressing of fresh and fresh sweet sorghum stalks: take 1 kg of fresh and sweet sorghum stalks grown in the field, and after peeling, obtain about 654 ml of sugar-containing juice and fiber residue after repeated pressing three times; Contains about 144g / L of free sugar. In addition, as measured by ICP-MS and Kjeldahl method, the contents of inorganic ions and nitrogen and phosphorus contained in the sugary juice are shown in Table 1:

[0071] Table 1 Inorganic ions and nitrogen and phosphorus contents in sugary juice from fresh and fresh sweet sorghum straw

[0072] element Content (mg / L) K 262.76 Na 17.6 Ca 143.86 Mg 263.45 Fe 2.07 mn 0.34 P 128.69 N 1480

[0073] (2) Degradation treatment of fiber residues: Dry the remaining about 294g fiber residues at 105°C overnight to dryness to obtain 216g...

Embodiment 2

[0081] Example 2: Production of ethanol by fermenting green and fresh sweet sorghum stalks as raw materials.

[0082]Steps (1), (2) are the same as in Example 1.

[0083] (3) Preparation of fermented liquid: 118.5 g of fiber residue after alkali degradation treatment is pulverized to 60 meshes, divided into 4 parts, and one part is fully mixed with the sugary juice prepared in step (1), and placed In addition, 1.7g / L urea, 1g / L potassium dihydrogen phosphate, and 2g / L anhydrous magnesium sulfate were added to the fermenter, and sterilized at 121°C for 25min to obtain a fermented liquid for later use.

[0084] (4) Preparation of seed liquid: the ethanol-producing strain is the high-temperature resistant Saccharomyces cerevisiae H5 screened by evolutionary engineering in the laboratory. The composition of the strain seed synthetic medium was (g / L): 0.8 sucrose, 20 glucose, 5 soybean peptone, 3 yeast powder, tap water, and the pH was adjusted to 5.5 with 1 mol / L HCl. The strain...

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Abstract

The invention relates to a method for producing ethanol by fermentation of fresh sugar-containing biomasses. The method comprises a step of adding pretreated residues of wood fiber solid materials of a fresh sugar-containing biomass source, cellulase and ethanol-producing strains into a sugar-containing juice of the fresh sugar-containing biomass source to perform fermentation, so as to obtain a fermented mash rich in ethanol, wherein the sugar-containing juice of the fresh sugar-containing biomass source and the wood fiber solid materials of the fresh sugar-containing biomass source are obtained by pressing fresh sugar-containing biomass raw materials. The method achieves simultaneous utilization of juice free sugar and cellulose sugar in a fresh sugar-containing plant raw material, improves the utilization rate of the biomass raw materials, and reduces the cost of raw materials for ethanol production; meanwhile, the concentration and the production efficiency of ethanol in a fermentation product are improved by adopting the fermentation mode, and the problems of low concentration and production efficiency of ethanol in a traditional fiber ethanol fermentation process are solved.

Description

technical field [0001] The invention belongs to the technical fields of fermentation engineering and biomass energy, and in particular relates to a method for producing ethanol by fermenting green and fresh sugar-containing biomass. Background technique [0002] With the development of science and technology economy, the dependence of human society on fossil energy is increasing day by day. The use of fossil fuels has problems such as high consumption and high pollution. Under the dual background of energy security and environmental protection, it is particularly necessary to develop sustainable, renewable, efficient, low-cost, convenient and safe energy sources. Biomass energy can effectively replace traditional fossil fuels and realize green and renewable energy. It is recognized as the only solution that can replace liquid fuels. Biofuel ethanol can reduce the consumption of gasoline and make gasoline burn more fully, thereby reducing the emission of harmful gases. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCC12P7/10Y02E50/10Y02P20/54
Inventor 谭天伟蔡的秦培勇张长伟文皓
Owner BEIJING UNIV OF CHEM TECH
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