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Fermentation method for improving yield of natamycin

A natamycin and fermentation method technology, which is applied in the field of fermentation to increase the yield of natamycin, can solve the problems of not reaching industrialization, high price, low yield and the like, and achieves reduction of feedback inhibition, improvement of recovery rate, and increase of yield Effect

Active Publication Date: 2017-05-31
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, natamycin still has the problems of low output and slow development of industrial production, which leads to its high price, so it is mainly used in high-end products, which limits the development of natamycin
Because of its low output, its price is relatively high, which seriously limits the industrial application of natamycin in my country
For a long time, most researchers have started to increase the yield of natamycin by conducting research on strain selection and optimization of fermentation process conditions, but the problem they are facing now is that it has not yet met the needs of industrialization

Method used

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  • Fermentation method for improving yield of natamycin

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Comparison scheme
Effect test

Embodiment 1

[0035] A fermentation method for improving the output of natamycin, the specific steps are as follows:

[0036] (1) Cell activation culture

[0037] Streptomyces natalis 4.3526 was cultured on a solid slant medium and cultured at 28°C for 7 days to obtain a slant strain of Streptomyces natalis 4.3526, which was stored at 4°C for future use. The formula of this solid slant medium is: distilled water 100mL, glucose 2g, agar 2g, tomato pulp 2g, oat 2g, pH value is natural.

[0038] (2) Shake flask seed culture

[0039]The Streptomyces natalal 4.3526 slant bacterial strain obtained in the step (1) is inoculated in the liquid seed medium with an inoculation loop in 2 loops, at 28 ℃, the shaker speed is cultivated for 2 days under the condition of 180r / min to obtain Streptomyces natalis 4.3526 seed liquid, spare. The above formula of the seed culture medium of Streptomyces natalis is: 10 g of glucose, 5 g of tryptone, 10 g of sodium chloride, 5 g of malt extract powder, 1 L of di...

Embodiment 2

[0047] A fermentation method for improving the output of natamycin, the specific steps are as follows:

[0048] (1) Cell activation culture

[0049] Streptomyces natalis 4.3526 is cultured on a solid slant medium and cultured at 30°C for 3 days to obtain a slant strain of Streptomyces natalis 4.3526, which is stored below 4°C for future use. The formula of this solid slant medium is: 100mL of distilled water, 1g of glucose, 1.5g of agar, 1.5g of tomato pulp, 2.5g of oatmeal, and the pH value is natural.

[0050] (2) Shake flask seed culture

[0051] The Streptomyces natalal 4.3526 slant bacterial strain obtained in the step (1) is inoculated in the liquid seed medium with an inoculation loop in 2 loops, and at 30° C., the shaking table speed is cultivated for 2.5 days under the condition of 180r / min to obtain Streptomyces natalis 4.3526 seed liquid, spare. The formula of the above liquid seed medium for Streptomyces natalis 4.3526 is: 8 g of glucose, 2 g of tryptone, 6 g of...

Embodiment 3

[0059] A fermentation method for improving the output of natamycin, the specific steps are as follows:

[0060] (1) Cell activation culture

[0061] Streptomyces natalis 4.3526 was cultured on a solid slant medium for 9 days at 26°C to obtain a slant strain of Streptomyces natalis 4.35264.3526, which was stored at 4°C for future use. The formula of this solid slant medium is: distilled water 100mL, glucose 1.5g, agar 1.5g, tomato pulp 2.5g, oat 3g, pH value is natural.

[0062] (2) Shake flask seed culture

[0063] The Streptomyces natalal 4.3526 slant bacterial strain obtained in the step (1) is inoculated in the liquid seed medium with an inoculation loop in 2 loops, and cultivated for 3 days at 26 ° C under the condition of a shaking table speed of 180 r / min to obtain Streptomyces natalis 4.3526 seed liquid, spare. The above formula of the seed culture medium of Streptomyces natalis is: 9g of glucose, 4g of tryptone, 8g of sodium chloride, 4g of malt extract powder, 1L o...

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Abstract

The invention provides a fermentation method for improving yield of natamycin. With streptomyces natalensis as a major raw material, the natamycin is obtained by sequentially conducting strain activation, seed culture, fermentation and natamycin extraction. Before fermenting the streptomyces natalensis, macroporous adsorption resin is added to a fermentation medium; when the streptomyces natalensis is fermented for 12-24h, soybean oil, which serves as an oxygen carrier, is added to fermentation liquid; and when the streptomyces natalensis is fermented for 24-48h, valine and threonine are added to the fermentation liquid in a fed-batch mode. With the application of the fermentation method provided by the invention, the yield of the natamycin can be improved by 3-4 times, namely to be about 4g / L, so that the yield of the natamycin is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of natamycin preparation, and in particular relates to a fermentation method for increasing the output of natamycin. Background technique [0002] Natamycin is an active polyolefin macrolide antifungal antibiotic, a secondary metabolite obtained by fermentation of Streptomyces natalis, and the molecular formula of natamycin is C 33 h 47 NO 13 , can contain 3 molecules of crystal water, and the relative molecular weight is 665.725; as shown in Formula 1, its chemical structure contains amino and carboxyl groups, so natamycin is an amphoteric substance, and there are usually two typical configurations in its molecular structure: ketone Formula structure and enol structure, among which the enol structure is mostly, which determines its low solubility in many solvents. Natamycin belongs to polyene macrolide compounds. The C3-C9 part of the lactone ring is a hemiacetal structure. The tetraene system is all-cis...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/465
CPCC12P19/626
Inventor 王大红韦兰兰张颖孙建瑞张孟娟宋鹏辉季鹏飞郑宇丹姚露露张帅滢
Owner HENAN UNIV OF SCI & TECH