Electrochemical sensor and method system for simultaneous detection of isocarbophos and acetamiprid

A technology of ammonium phosphine and acetamiprid, which is applied in the field of sensors and can solve the problems of complicated instrument operation and inability to achieve high throughput and the like

Active Publication Date: 2019-06-14
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims at the shortcomings of complex instrument operation, professional operators and inability to achieve high throughput in the existing detection methods, and provides an electrochemical sensor and its method system for simultaneously detecting isocarbophos and acetamiprid. Electrochemical Detection of Isocarbophos and Acetamiprid by Ligand

Method used

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  • Electrochemical sensor and method system for simultaneous detection of isocarbophos and acetamiprid
  • Electrochemical sensor and method system for simultaneous detection of isocarbophos and acetamiprid
  • Electrochemical sensor and method system for simultaneous detection of isocarbophos and acetamiprid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] a. The gold electrode was first polished in an alumina slurry with a particle size of 0.3 μm and an alumina slurry with a particle size of 0.05 μm until it became a mirror surface, and the electrode was immersed in 20 mL of H with a volume ratio of 3:1. 2 SO 4 and H 2 O 2 In the mixed solution, incubate for 20min and wash thoroughly with ultrapure water;

[0073] b. Add a total of 6 μL of XB solution (10 μM XB1: 2 μL and 10 μM XB2: 4 μL) dropwise to the electrode surface treated in step a, incubate at room temperature for 2 h, the beacon probe XB converts sulfhydryl groups through Au-S bonds The chain is fixed to the electrode surface.

[0074] c. Use MCH to block unspecific binding sites, specifically: drop 6 μL of 1 mM MCH solution onto the electrode surface, incubate for 30 min, and wash with PBS.

[0075] d, carry out homogeneous reaction, the main steps are:

[0076] 1. Add 2μL of 100nM HAP1, 2μL of 100nM HAP2 and 20μL of 1× Phi29 DNA polymerase buffer solutio...

Embodiment 2

[0084] a. The gold electrode is first polished in an alumina slurry with a particle size of 0.3 μm and an alumina slurry with a particle size of 0.05 μm until it becomes a mirror surface, and the electrode is immersed in 20 mL of H with a volume ratio of 3:1. 2 SO 4 and H 2 O 2 The mixture was incubated for 20 min and washed thoroughly with ultrapure water;

[0085] b. Add a total of 6 μL of XB solution (10 μM XB1: 2 μL and 10 μM XB2: 4 μL) dropwise to the electrode surface treated in step a, incubate at room temperature for 2 h, the beacon probe XB converts sulfhydryl groups through Au-S bonds The chain is fixed to the electrode surface.

[0086] c. Modify MCH to block unspecific binding sites.

[0087]d, the main steps in the homogeneous reaction:

[0088] 1. Add 2μL of 100nM HAP1, 2μL of 100nM HAP2, and 20μL of 1× Phi29 DNA polymerase buffer solution to the centrifuge tube, add excess ammonium phosphine (500ppb), and incubate at 37°C for 30min;

[0089] 2. Continue to...

Embodiment 3

[0095] a. The gold electrode is first polished in an alumina slurry with a particle size of 0.3 μm and an alumina slurry with a particle size of 0.05 μm until it becomes a mirror surface, and the electrode is immersed in 20 mL of H with a volume ratio of 3:1. 2 SO 4 and H 2 O 2 In the mixed solution, incubate for 20min and wash thoroughly with ultrapure water;

[0096] b. Add a total of 6 μL of XB solution (10 μM XB1: 2 μL and 10 μM XB2: 4 μL) dropwise to the electrode surface treated in step a, incubate at room temperature for 2 h, the beacon probe XB converts sulfhydryl groups through Au-S bonds The chain is fixed to the electrode surface.

[0097] c. Modify MCH to block unspecific binding sites.

[0098] d, the main steps in the homogeneous reaction:

[0099] 1. Add 2μL of 100nM HAP1, 2μL of 100nM HAP2 and 20μL of 1× Phi29 DNA polymerase buffer solution to the centrifuge tube, add excess acetamiprid (500ppb), and incubate at 37°C for 30min;

[0100] 2. Continue to add...

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Abstract

The invention discloses an electrochemical sensor and a method system for simultaneously detecting isocarbophos and acetamiprid. From the inside to the outside, there are electrodes, beacon probe layer, 6-mercaptohexa-1-alcohol (MCH) sealing layer, and homogeneous reaction product layer. Blue and ferrocene modification; the preparation method is first to polish the electrode and treat it with H2SO4 and H2O2, and then modify the beacon probe on the surface of the electrode. The sensor of the invention has simple preparation method, stable performance and good electrode repeatability, and is suitable for the detection of isocarbophos and acetamiprid in food safety and the practical application of biosensor industrialization; it can realize the detection of isocarbophos and acetamiprid in food. In the rapid online detection of acetamiprid, the detection limits of isocarbophos and acetamiprid were 0.12 and 0.37ppb, respectively.

Description

technical field [0001] The invention relates to a method for detecting two pesticides in the technical field of sensors, in particular to an electrochemical sensor and a method system for simultaneously detecting amiphos and acetamiprid. Background technique [0002] Organophosphorus is widely used for insecticide, sterilization and weeding of crops, and it is the most widely used pesticide in my country. Organophosphorus pesticides are nerve poisons, which mainly inhibit the activity of acetylcholinesterase in blood and tissues, resulting in a large accumulation of acetylcholine, thereby blocking nerve conduction and causing central nervous system poisoning. Aminophos is a highly toxic organophosphorus insecticide, which can cause poisoning through the esophagus, skin and respiratory tract. As a highly toxic pesticide, Amphos is prohibited from being used in fruit, tea, tobacco, vegetables, Chinese herbal medicine and other plants or vegetables and fruits. Acetamiprid is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/38G01N27/26
CPCG01N27/26G01N27/30G01N27/38
Inventor 徐霞红郭玉娜王新全王祥云王强杨华齐沛沛汪志威
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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