Biochemical process of governing soil cadmium pollution
A cadmium pollution and soil technology, applied in the biological field, can solve the problems of inability to fix cadmium metal, loss of cadmium metal, poor stability, etc., and achieve the effects of eliminating cadmium pollution, low restoration cost, and high restoration efficiency
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Embodiment 1
[0022] A biochemical process for controlling soil cadmium pollution, comprising the steps of:
[0023] 1) Preparation of microbial preparations: respectively culture Phanerochaete chrysosporium ATCC 24725 and Pseudomonas CCTCC NO: 2011184 (see CN2011102153648) to a concentration of 1×10 8 Bacterial solution per ml, mixed according to the volume ratio of 2:1 to obtain a mixed bacterial solution, and then the mixed bacterial solution and diatomaceous earth were mixed and stirred evenly according to a mass ratio of 1:2, and finally low-temperature drying was carried out at a drying temperature of 15°C , the moisture content after drying is 8wt%, namely;
[0024] 2) Preparation of nano-iron preparation: under the protection of nitrogen, feed FeSO with a concentration of 0.02M 4 Add polyvinylpyrrolidone to the aqueous solution, and the amount of polyvinylpyrrolidone added is FeSO 4 1% of the mass of the solution; stir mechanically to make it fully mixed, and the mixing time is 15...
Embodiment 2
[0029] A biochemical process for controlling soil cadmium pollution, comprising the steps of:
[0030] 1) Preparation of microbial preparations: Phanerochaete chrysosporium ATCC 24725 and Pseudomonas CCTCC NO: 2011184 were cultured to a concentration of 1×10 8 bacteria solution per ml, mixed according to the volume ratio of 3:2 to obtain the mixed bacteria solution, then mixed with diatomaceous earth according to the mass ratio of 1:2 and stirred evenly, and finally dried at low temperature, the drying temperature was 15°C, and after drying, it contained The amount of water is 8wt%, to get final product;
[0031] 2) Preparation of nano-iron preparation: under the protection of nitrogen, feed FeSO with a concentration of 0.02M 4 The polyvinylpyrrolidone added in the solution, the amount of polyvinylpyrrolidone added is FeSO 4 1% of the solution mass; mechanically stir to make it fully mixed, the mixing time is 15min, and then add the same volume of NaBH with a concentration o...
Embodiment 3
[0036] Migration and stability test: the nano-iron preparation prepared in Example 1 was used as the experimental group, and the preparation not modified by polyvinylpyrrolidone was selected as the control group (the preparation method was the same as in Example 1, without adding polyvinylpyrrolidone).
[0037] Proceed as follows:
[0038] (1) Fe in the experimental group and the control group 0 The concentration is 1.0g / L.
[0039] (2) Take 10 g of cadmium-contaminated acidic soil and fill them in resin glass columns with a diameter of 1.0 cm and a length of 10 cm.
[0040] (3) The same volume of Fe 0 The solution and purified water were injected from the top of the soil column at a rate of 0.2 mL / min, and the penetrating fluid was collected.
[0041](4) The penetration solution was filtered through a 0.22 µm filter membrane, 0.1 M HCl was added, and the Fe content was determined by ion plasma-mass spectrometer (ICP-MS); Fe content (mg L -1 ) see Table 1 for details:
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