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Culture medium capable of promoting growth of phellinus igniarius mycelia

A technology for the growth of Phellinus linteus mycelium and mycelium, which is applied in a method based on microorganisms, uses added compounds to stimulate growth, microorganisms and other directions, can solve problems such as insufficient supply of Phellinus linteus mycelium, and achieves low price and accelerated growth rate. , the effect of reducing costs

Inactive Publication Date: 2017-06-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect of existing culture medium formula, provide a kind of method that can promote the growth of Phellinus mycelia, to solve the defect of insufficient supply of Phellinus mycelium, improve the output of Phellinus polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Optimization test of carbon source and nitrogen source

[0015] Carbon source: add 2% glucose, fructose, maltose, sucrose, soluble starch, corn flour, and flour to the basal medium as carbon sources to form seven different carbon source culture media, and use the basal medium as a carbon-free source The control group was used to study the effects of different carbon sources on the growth of Phellinus mycelium.

[0016] The study found that maltose was the most suitable carbon source for Phellinus fungus. The effect of maltose as carbon source is the best, the mycelial growth rate is 2,89mm / d, the mycelial diameter is 42.59mm, and the mycelial growth index is 13.50. From the analysis of mycelial growth index, the colony diameter of maltose was significantly increased compared with the control group.

[0017] Nitrogen source: Add 0.3% peptone, yeast powder, soybean powder, ammonium tartrate, ammonium sulfate, and ammonium nitrate as nitrogen sources to the bas...

Embodiment 2

[0020] A kind of culture medium that promotes Phellinus mycelia growth, its medium formula is as follows: maltose 16g / L; Ammonium tartrate 0.25g / L; KH 2 PO 4 0.8g / L; MgSO 4 •7H 2 O 0.3g / L; VB 1 8mg / L; agar 18g / L.

[0021] The configuration method is: weigh maltose, ammonium tartrate, KH 2 PO 4 , MgSO 4 •7H 2 O. VB 1 , dissolved in 1L of water and added, fully stirred evenly, added agar and heated and stirred until the agar melted; divided into triangular flasks, sealed with a parafilm at the bottle mouth, and sterilized at 121°C for 30 minutes for later use.

[0022] Phellinus cultivation:

[0023] (1) Inoculation: when the temperature of the medium drops to 36°C, inoculate Phellinus Phellinus, seal it with a parafilm and place it in an incubator for cultivation;

[0024] (2) Mycelia growth: Phellinus japonica grew on the above medium for a total of 10 days. The line was drawn on the third day after inoculation, and then drawn once every 24 hours, and the line was ...

Embodiment 3

[0026] A kind of culture medium that promotes Phellinus mycelia growth, its culture medium formula is as follows: maltose 18g / L; Ammonium tartrate 0.28g / L; KH 2 PO 4 1.0g / L; MgSO 4 •7H 2 O 0.4g / L; VB 1 10mg / L; agar 20gg / L.

[0027] The configuration method is: weigh maltose, ammonium tartrate, KH 2 PO 4 , MgSO 4 •7H 2 O. VB 1 , dissolved in 1L of water and added, fully stirred evenly, added agar and heated and stirred until the agar melted; divided into triangular flasks, sealed with a parafilm at the bottle mouth, and sterilized at 121°C for 30 minutes for later use.

[0028] Phellinus cultivation:

[0029] (1) Inoculation: When the temperature of the medium drops to 38°C, inoculate Phellinus Phellinus, seal it with a parafilm and place it in an incubator for cultivation;

[0030] (2) Mycelia growth: Phellinus japonica grew on the above medium for a total of 10 days. The line was drawn on the third day after inoculation, and then drawn once every 24 hours, and the ...

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Abstract

The invention provides a culture medium capable of promoting the growth of phellinus igniarius mycelia. The culture medium is prepared by taking maltose as a best carbon source for culturing the phellinus igniarius mycelia, using ammonium tartrate as a best nitrogen source for culturing the phellinus igniarius mycelia, and selecting high-purity chemical reagents prepared from accurate components for use, so that the components of the culture medium are enabled to be accurate, and the strain quality control is facilitated. The culture medium is optimized according to the nutritional ingredients needed by the growth of the phellinus igniarius mycelia, thus accelerating the growth speed of the phellinus igniarius mycelia; the phellinus igniarius mycelia can overgrow on an oblique plane after being inoculated for 7-9 days; compared with a potato dextrose agar (PDA) culture medium, the culture medium provided by the invention accelerates the mycelial growth speed by about 20%. The culture medium shortens the growth time of the phellinus igniarius mycelia, is simple in formula, wide in material source and low in price, can reduce the production cost of phellinus igniarius, and provides reliable technical support for the subsequent application of the phellinus igniarius.

Description

technical field [0001] The invention relates to the field of cultivation of edible and medicinal fungi, in particular to a method for promoting the growth of Phellinus mycelium by optimizing the culture medium. Background technique [0002] Phellinus japonica is a precious perennial large medicinal fungus, which gets its name because most of them grow on Morus plants and the fruiting bodies are yellowish brown. The medicinal effects of Phellinus Phellinus were first seen in "Compendium of Materia Medica" and "On the Properties of Medicine", which recorded that Phellinus Phellinus tasted bitter and cold in nature. In traditional Chinese medicine in my country, it is used to treat symptoms such as dysentery, night sweats, metrorrhagia, and prolapse of the anus. The latest research shows that Phellinus also has a unique anti-cancer effect, and it is a medicinal fungus with the highest efficiency among the internationally recognized biological cancer treatment agents. The main...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/14C12R1/645
CPCC12N1/38C12N1/14
Inventor 傅俊生李佳欢戚梦胡开辉林文雄
Owner FUJIAN AGRI & FORESTRY UNIV
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