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Stimuli-responsive type nucleic acid nanostructure carrier chiral noble metal nanocomposite and preparation method thereof and application

A nanocomposite, nucleic acid nanotechnology, applied in the fields of nanotechnology, nanotechnology, DNA preparation, etc., can solve problems such as limited application

Active Publication Date: 2017-06-27
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the accumulation of chains and the limited conformational adjustment ability of these methods, the application of plasma structures in response to biologically active substances in complex biological environments is limited.

Method used

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  • Stimuli-responsive type nucleic acid nanostructure carrier chiral noble metal nanocomposite and preparation method thereof and application
  • Stimuli-responsive type nucleic acid nanostructure carrier chiral noble metal nanocomposite and preparation method thereof and application
  • Stimuli-responsive type nucleic acid nanostructure carrier chiral noble metal nanocomposite and preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of nucleic acid nanostructure carrier, gold nanorod, nucleic acid nanostructure carrier-gold nanorod plasmonic chiral complex

[0062] Mix the scaffold chain:staple chain:capture chain:control chain in a molar ratio of 1:10:10:10 at 1×TAE / Mg 2+ Annealing and hybridization in PCR under the buffer system to obtain triangles I and II. The annealing condition is: slowly drop from 90°C to room temperature, and the whole process lasts for 12h. Triangles I and II were purified with a 100kD ultrafiltration column to remove excess DNA strands. Then, triangles I and II were mixed at a molar ratio of 1:1, annealed and hybridized in PCR, and the annealing conditions were: from 45°C to 25°C as one cycle, a total of 6 cycles. The triangular DNA origami used in this experiment was modified based on Rothemund's 2006 design (Nature, 2006, 440, 297-302.), and the capture strand was extended at 7 positions (5'-AAAAAAAAAAAAAAAA-original staple strand sequence -3'),...

Embodiment 2

[0074] Example 2 Glutathione Regulates Optical Chiral Signal of Stimuli-Responsive Nucleic Acid Nanostructure Carrier Gold Nanorod Complex

[0075] A disulfide bond was introduced on the control chain connecting triangles I and II, and the assembled diamond-shaped origami structure was combined with gold nanorods to form a corresponding complex. The complex was mixed with different concentrations (1, 5, 10 mM) of glutathione Peptides were incubated at 25°C for 12 h, and then separated by electrophoresis to detect the dissociation of the complex; the optical changes of the complex were detected by circular dichroism.

[0076] Circular dichroism characterization: use Jasco J-1500 circular dichroism spectrometer to characterize the chiral signal of the sample. The whole process of the instrument is under the protection of nitrogen. The scanning temperature is room temperature, the wavelength range is 500-900nm, and the cuvette light The distance is 0.5cm, the scan speed is 200nm / ...

Embodiment 3

[0078] Example 3 Restriction nuclease EcoR V regulates the optical chiral signal of the stimulus-responsive nucleic acid nanostructure carrier-gold nanorod complex

[0079] Introduce the restriction endonuclease specific recognition restriction endonuclease sequence on the control strand connecting triangles I and II The diamond-shaped origami structure assembled by introducing enzyme-cut sequences I and II was combined with gold nanorods to form a corresponding complex. After incubating the complex with EcoR V for 1 h at 37°C, electrophoresis separation was performed to detect the solution of the complex. dissociation; optical changes of the complexes were detected by circular dichroism spectroscopy.

[0080] Circular dichroism characterization: use Jasco J-1500 circular dichroism spectrometer to characterize the chiral signal of the sample. The whole process of the instrument is under the protection of nitrogen. The scanning temperature is room temperature, the wavelength r...

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Abstract

The invention discloses a nucleic acid nanostructure carrier, a stimuli-responsive type nucleic acid nanostructure carrier chiral noble metal nanocomposite comprising the same and a preparation method thereof and application. The nucleic acid nanostructure carrier is of two-triangular DNA paper folding structure constructed through a DNA paper folding technology, and the two triangular DNAs are connected through a control chain to form a rhombic paper folding structure. Concretely, the nucleic acid nanostructure carrier is of a nucleic acid nano-structure formed by self-assembly through hybridization of a scaffold chain and an auxiliary folding staple chain and hybridization of a control chain and the scaffold chain of the two-triangular DNA paper folding structure respectively. On the one hand, the stimuli-responsive type nucleic acid nanostructure carrier chiral noble metal nanocomposite overcomes shortcomings of optical signal modulation through a conventional plasma assembly (chain accumulation and limited adjustment range); on the other hand, a plasma structure is adjusted through an external environment, and the nucleic acid nanostructure carrier can be used for monitoring specific bioactive substances and the like.

Description

technical field [0001] The invention belongs to the field of nanocomposite materials, and in particular relates to a stimulus-responsive nucleic acid nanostructure carrier chiral noble metal nanocomposite and its preparation method and application. Background technique [0002] In organisms, the stimuli-responsive biomacromolecular assembly system plays a very important role. The higher the evolutionary level of life, the stress response to environmental changes will be faster and more accurate. This rapid stress behavior is the result of the joint function of every cell that constitutes the life. When the environment around the cell changes, the cell can produce corresponding biological functions by changing the conformation of the biomacromolecular assembly system to better adapt to the environment. Inspired by nature, a large number of artificially assembled structures with stimuli-responsive functions have been prepared in large numbers. [0003] When the incident ligh...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10G01N21/59G01N21/25B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00C12N15/10C12N15/11G01N21/25G01N21/59G01N2021/258G01N2021/5903
Inventor 丁宝全李彤彤蒋乔刘清王振刚湛鹏飞宋琳琳王婷张莉刘鸣华
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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