Fabrication method of microfluidic chip based on nanofiber template method

Abstract

The invention discloses a micro-fluidic chip manufacturing method based on the nanofiber template method. Firstly, a receiving plate is coated with a photo-curing material layer to form a micro-fluidic chip substrate; secondly, near-field electrospinning or micro liquid spinning is conducted to obtain a first fiber layer with a certain pattern; thirdly, the receiving plate descends by a certain height along with a moving platform, orderly sedimentation of a second fiber layer is conducted, and spinning and curing are conducted layer by layer to obtain a photo-curing model combining orderly fibers with a photo-curing material; fourthly, a last fiber layer is communicated with the surface of the photo-curing model, and when the fibers are completely wrapped by the photo-curing material and certain allowance is reserved, forming of the photo-curing model is completed; fifthly, the photo-curing model is taken out and placed into a solvent, and ultrasonic shaking soluble fibers are added; sixthly, when micro-nanofibers are completely dissolved, the model is taken out to obtain a micro-fluidic chip. The soluble fibers serve as channel filler, and forming precision can reach the precision level same as that of the fiber diameter.

Description

technical field [0001] The invention relates to a method for manufacturing a microfluidic chip by using a nanofiber template, in particular to a method for manufacturing a microfluidic chip with nano-channels or complex three-dimensional channels, and belongs to the technical field of micro-nano processing. Background technique [0002] Microfluidic chips are based on the basic principles and technologies of ordinary capillary electrophoresis, and use micro-nano processing technology to process various microstructures on silicon, quartz, glass or polymer matrix materials, such as pipes and reaction pools. , electrodes and other functional units to complete a series of tasks involved in the fields of biology and chemistry, such as sample preparation, biochemical reactions, processing (mixing, filtration, dilution), separation and detection, etc., with fast, efficient, low consumption, and analysis process A miniature analytical experimental device with the characteristics of ...

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Problems solved by technology

[0003] Traditional microfluidic chip microchannel molding processing is mainly injection molding and thermocompression molding, such as Chinese patents 201110072477.7, 201010119177.5, 200910308065.1, 201010300409.7, which provide various microfluidic chip injection molds with microchannel structures on the molding surface, However, due to the micro-scale effect, there are problems of difficult filling and molding of micro-channels, and the manufacturing of injection molds for microfluidic chips is difficult, the injection molding process is complicated, and the production cost is high; thermocompression molding has long heating time, high energy consumption, and high production costs. Problems such as high pressure, thermal deformation and limited forming accuracy

[0004] In addition, methods such as micro-cutting and laser forming have limitations in the scope of processing and conditions. For example, the processing efficiency of micro-cutting is low, and the size and precision of the micro-channels are limited; although laser micro-etching has high processing efficiency, it is The equipment is expensive, the precision of the processed surface is not high, and the wall surface of the processed microstructure channel is rough. This is because residual waste and protrusions are easily adhered to the wall surface of the microchannel, such as literature (CHINESE JOURNAL OF LASERS May.Vol.36, No.5, 2009) reported the phenomenon of recasting in CO2 laser-assisted processing of PMMA microfluidic chips

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