Method for directional domestication of inoculum applicable to anaerobic digestion of straw for production of biogas

A technology of anaerobic digestion and inoculum, applied in the direction of using microorganisms, etc., can solve the problems of difficult cultivation of special inoculum, high transportation cost, long start-up time, etc., achieve significant gas production effect, easy to obtain, and solve the problem of domestication long time effect

Inactive Publication Date: 2017-07-11
NANKAI UNIV
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Problems solved by technology

[0005] The invention relates to a method for directional domestication inoculum suitable for anaerobic digestion of crop straws for biogas production, and aims to solve the p

Method used

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Examples

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Embodiment Construction

[0024] The microcrystalline cellulose used in the following examples is a conventional chemical used in the laboratory; corn stalks are obtained from a corn field in Jinghai County, Tianjin City after normal harvesting, air-dried and pulverized naturally. The specific domestication process is as follows:

[0025] (1) Pour 6 L of sludge anaerobically digested from an urban sewage treatment plant into a fermenter, of which the total volume of the fermenter is 10 L, the effective volume is 6 L, and the upper part of the system is fed and the bottom is discharged; The TS% of the anaerobic digested sludge is 6.46%, the VS% is 3.63%, the pH value is 7.2, the C% is 29.78%, and the N% is 5.08%;

[0026] (2) After the anaerobic digested sludge was introduced, the system did not add any nutrients for the first 7 days, and the inoculum was allowed to adapt to the new environment at 37±0.5°C, while consuming excess organic matter inside the system. Among them, the methane content ( 47%),...

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Abstract

The invention relates to a method for directional domestication inoculum suitable for anaerobic digestion of straw to produce marsh. Among them, microcrystalline cellulose is used instead of crop straw, dewatered sludge or livestock and poultry manure as domestication substrate, and an inoculum suitable for anaerobic digestion of straw to produce biogas is obtained through a domestication process of hydraulic retention time. The domestication content includes: (1) Referring to the semi-continuous operation conditions of the straw biogas demonstration project, first design the hydraulic retention time of the system, the inflow and outflow volume, and the TS% value of the system operation; (2) After the system is connected to the original bacterial source, let the inoculum adapt to the System for a few days, and then start acclimatization; (3) According to the designed system operation TS% value and the value of inflow and outflow, make a mixed solution of microcrystalline cellulose and water in a certain proportion; (4) Regular discharge and feed every day , to carry out a domestication process of one HRT; (5) Obtain the inoculum for directional domestication after the domestication is over. This technology can not only shorten the cultivation time of the inoculum, reduce the transportation cost of the inoculum, and simplify the domestication process, but also effectively improve the gas production efficiency of crop straw and shorten the start-up time of the device, which can provide a reference for straw biogas demonstration projects.

Description

technical field [0001] The invention relates to the field of microorganism application agriculture, in particular to a method for directional domestication inoculum suitable for anaerobic digestion of straw to produce marsh. Background technique [0002] In recent years, with the international energy shortage and environmental pollution becoming more and more serious, the use of anaerobic digestion methane production technology to convert crop straw into biogas not only realizes turning waste into wealth, but also produces clean energy gas, realizing the conversion of biomass solid waste. Resource utilization and energy utilization not only ease the energy crisis in our country, but also ease the contradiction between rural economic development and energy and environment. [0003] There are many factors affecting the gas production of anaerobic digestion, among which the inoculum is very important for the anaerobic digestion process of crop straw and methane production. How...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/22
CPCC12N1/36C12N1/22
Inventor 杨茜鞠美庭李维尊
Owner NANKAI UNIV
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