Preparation method of hard tissue engineering scaffold with gene regulation function

A gene regulation and hard tissue technology, applied in tissue regeneration, textiles and papermaking, medical science, etc., can solve the problems that it is difficult to meet the design requirements of scaffold materials, and the precise regulation of cell growth genes cannot be realized.

Active Publication Date: 2017-07-14
杭州昊莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to meet the design requirements of scaffold materials simply by adding growth factors, let alone the precise regulation of cell growth genes, and it is necessary to develop new cell growth regulation methods

Method used

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  • Preparation method of hard tissue engineering scaffold with gene regulation function
  • Preparation method of hard tissue engineering scaffold with gene regulation function

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) in CaO-P 2 o 5 -SiO 2 P123 was added to the system, and the parameters of the electrospinning process were: electrostatic voltage 10Kv, receiving distance 10cm, spinning rate 1ml / h, relative humidity 25%. The high-speed rotating drum rotates at 200 rpm to obtain regularly arranged micro-nano fibers; the fibers are sintered at a temperature of 300 ° C for 120 minutes to make the surfactant on the fibers evaporate violently, thereby forming a pore structure on the surface of the fibers and obtaining mesoporous organisms Glass micro-nanofibers.

[0030] (2) Extract the Osterix target gene fragment, construct the target gene plasmid, and obtain the plasmid DNA recombinant by gene recombination technology. Mix the chitosan solution and plasmid DNA recombinant, the mixing mass ratio range is 2:1, utilize the attraction between chitosan molecules and DNA, form plasmid nano-microspheres under the condition of rapid stirring at 50 rpm .

[0031] (3) Mix the biological g...

Embodiment 2

[0033] (1) in CaO-P 2 o 5 -SiO 2P123 was added to the system, and the parameters of the electrospinning process were: electrostatic voltage 15Kv, acceptance distance 15cm, spinning rate 2ml / h, relative humidity 30%. The high-speed rotating drum rotates at 300rpm to obtain regularly arranged micro-nano fibers; the fibers are sintered at a temperature of 300°C for 120 minutes to make the surfactant on the fibers evaporate violently, thereby forming a pore structure on the surface of the fibers and obtaining mesoporous organisms Glass micro-nanofibers.

[0034] (2) Extract the Osterix target gene fragment, construct the target gene plasmid, and obtain the plasmid DNA recombinant by gene recombination technology. Mix the chitosan solution and plasmid DNA recombinant, the mixing mass ratio range is 3:1, utilize the attractive force between chitosan molecules and DNA, form plasmid nano-microspheres under the condition of rapid stirring at 80 rpm .

[0035] (3) The biological gl...

Embodiment 3

[0037] (1) in CaO-P 2 o 5 -SiO 2 P123 was added to the system, and the parameters of the electrospinning process were: electrostatic voltage 20Kv, acceptance distance 20cm, spinning rate 5ml / h, relative humidity 50%. The high-speed rotating drum rotates at 400rpm to obtain regularly arranged micro-nano fibers; the fibers are sintered at a temperature of 380°C for 150 minutes to make the surfactant on the fibers evaporate violently, thereby forming a pore structure on the surface of the fibers and obtaining mesoporous organisms Glass micro-nanofibers.

[0038] (2) Extract the Osterix target gene fragment, construct the target gene plasmid, and obtain the plasmid DNA recombinant by gene recombination technology. Mix the chitosan solution and plasmid DNA recombinant, the mixing mass ratio range is 3:1, utilize the attractive force between chitosan molecules and DNA, form plasmid nano-microspheres under the condition of rapid stirring at 80 rpm .

[0039] (3) The biological g...

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Abstract

The invention discloses a preparation method of a hard tissue engineering scaffold with a gene regulation function. The preparation method comprises the steps of adopting a method combining a sol-gel technology and an electro-spinning technology for preparing CaO-P2O5-SiO2 system mesoporous bioactive glass micro-nanofiber as an inorganic base phase; utilizing a gene recombination technology for preparing plasmid DNA (Deoxyribonucleic Acid) loaded with an osteocyte regulatory factor Osterix target gene, and using chitosan for embedding the plasmid DNA to prepare into a microballoon as an additive phase; preparing an emulsion system formed by the bioactive glass micro-nanofiber, the genetic vector microballoon, PCL and other materials, utilizing a thermally induced phase separation method for preparing a bioactive glass/PCL bionicbone tissue engineering scaffold compounding the Osterix plasmid DNA, and obtaining the hard tissue engineering scaffold with the gene regulation function. The hard tissue engineering scaffold can stimulate cells on a molecular level and produces a special response so as to realize accurate regulation on cell growth genes.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold materials, and relates to a preparation method of a hard tissue engineering scaffold, in particular to a preparation method of a hard tissue engineering scaffold with gene regulation function. Background technique [0002] Bone defect refers to the bone loss caused by congenital or acquired diseases, trauma and population aging. It is a serious disease that threatens human health and life. The research on bone defect repair has been one of the key topics that people have been paying close attention to for a long time. one. The effective methods for treating bone defects are autologous bone graft, allogeneic bone graft and artificial bone graft. Traditional autologous bone grafting is still the most ideal bone grafting material and the "gold standard" of bone grafting, but it suffers from many limitations, such as limited bone source, damage to the donor site, insufficient amount of bone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/10A61L27/54A61L27/56D01D5/00
CPCA61L27/10A61L27/18A61L27/54A61L27/56A61L2300/258A61L2300/412A61L2300/60A61L2430/02D01D5/0007D01D5/0076C08L67/04
Inventor 陈晓峰曹晓东原波孙璐瑶
Owner 杭州昊莱生物科技有限公司
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