Rhodococcus pyridinovorans and application thereof in microbial fuel cell

A technology of Rhodococcus pyridinephilus and Rhodococcus pyridine, which is applied in the field of Rhodococcus pyridinephilus and its application in microbial fuel cells, can solve the problems that the electricity production activity of Rhodococcus pyridinephilus has not been found, and achieve low battery operation cost, Improve the output voltage, the effect of good adaptability

Active Publication Date: 2017-07-21
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the literature and patents, no reports on the electrogenic activity of Rhodococcus pyridinephila have been found

Method used

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  • Rhodococcus pyridinovorans and application thereof in microbial fuel cell
  • Rhodococcus pyridinovorans and application thereof in microbial fuel cell
  • Rhodococcus pyridinovorans and application thereof in microbial fuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation and identification of Rhodococcus pyridinovorans HR-1

[0031] Using the landfill leachate from a waste incineration plant in Guangdong Province as the anolyte of a single-chamber air-cathode microbial fuel cell, the single-chamber air-cathode microbial fuel cell was started. The single-chamber air-cathode microbial fuel cell uses carbon cloth as the cathode and anode electrodes, Pt / C as the cathode catalyst, and an external 1000Ω resistor. The single-chamber air-cathode microbial fuel cell was placed in a biochemical incubator at 30°C for static cultivation. Monitor the output voltage of the battery online, and the maximum output voltage reaches 0.35V. After the output voltage of the battery is stable, continue to cultivate for one month, and the output voltage of the battery is stable (see figure 1 ).

[0032] Disassemble the battery, cut a small piece of anode carbon cloth, insert it into LB liquid medium for expansion culture, and then apply L...

Embodiment 2

[0044] Embodiment 2: take sodium acetate as the electricity production situation of fuel

[0045] The Rhodococcus pyridinovorans (Rhodococcus pyridinovorans) HR-1 obtained in Example 1 was inoculated into LB liquid medium, and the bacteria were activated on a shaking table at 30°C and 160 rpm for 36 hours, so that the number of bacteria reached the exponential growth phase, and the bacteria The solution was centrifuged at 4000rpm for 10 minutes, washed three times with PBS, and resuspended in 0.2mol / L sodium acetate solution (that is, the anolyte containing fuel) as the anode inoculum of the microbial fuel cell.

[0046] The cathode and anode electrodes are made of carbon cloth, Pt / C is the cathode catalyst, and a single-chamber air cathode microbial fuel cell with an external resistance of 1000Ω is assembled and sterilized, and then the anode inoculum prepared above is inserted into the battery to shorten the battery life Start-up time, the number of bacteria in the anode ino...

Embodiment 3

[0047] Example 3: Electricity production using glucose as fuel

[0048] The difference with embodiment 2 is:

[0049] The anolyte containing fuel is a 0.2mol / L glucose solution, and the number of Rhodococcus pyridinovorans HR-1 cells in the anode inoculum reaches 1×10 6 cells / L, the battery was placed in a biochemical incubator at 30°C for static cultivation, and the maximum output voltage of the battery was stabilized at 0.40V by online monitoring, indicating that Rhodococcus pyridinovorans (Rhodococcus pyridinovorans) HR-1 can be effectively degraded in a microbial fuel cell system Glucose gets electricity.

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Abstract

The invention discloses Rhodococcus pyridinovorans and application thereof in a microbial fuel cell. The Rhodococcus pyridinovorans HR-1 is preserved in China General Microbiological Culture Collection Center (CGMCC) in No.3 Microbiology Research Institute of China Science Academy, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, on April13th, 2017, with a preservation number of CGMCC NO.14021. The Rhodococcus pyridinovorans HR-1 disclosed by the invention is facultative anaerobe, has the functions of effectively decomposing multiple organic matters and obtaining electric energy at the same time and improving the output voltage of the microbial fuel cell, has better adaptability to extreme organic wastewater such as landfill leachate and industrial wastewater, and can be used as an anode catalyst of the microbial fuel cell for treating the extreme organic wastewater.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of environment and new energy, and in particular relates to a rhodococcus pyridineophilus and its application in microbial fuel cells. [0003] Background technique: [0004] A microbial fuel cell (MFC) is a device that uses microorganisms as an anode catalyst to directly convert chemical energy in organic matter into electrical energy. Compared with conventional fuel cells, MFCs use microorganisms instead of expensive chemical catalysts, and have many advantages such as mild operating conditions, no need to purify fuel to prevent catalyst poisoning, environmental friendliness, high energy conversion rate, good biocompatibility, and wider source of raw materials. . In theory, all microbially degradable organic matter (such as organic waste water, human and animal manure, kitchen waste, etc.) can be used as fuel for MFCs, so MFCs can generate clean energy while disposing of organic waste. [0005] As...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCC12N1/205C12R2001/01H01M8/16Y02E60/50
Inventor 袁浩然邓丽芳程鹏阮颖英呼和涛力
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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