A kind of Pacific oyster neutral polysaccharide and its preparation method and application

A technology of Pacific oyster and neutral polysaccharide, which is applied in pharmaceutical formulations, allergic diseases, organic active ingredients, etc., can solve the problems of many operation steps, low product yield, reduced activity, etc., and achieves promotion of immune activity and simple extraction process. , the effect of inhibiting proliferation

Active Publication Date: 2019-03-22
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In short, the above methods for extracting and removing impurities have problems such as many operation steps, low product yield, long operation cycle, introduction of impurities, and reduced activity.

Method used

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  • A kind of Pacific oyster neutral polysaccharide and its preparation method and application
  • A kind of Pacific oyster neutral polysaccharide and its preparation method and application
  • A kind of Pacific oyster neutral polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Pacific oyster neutral polysaccharide finished product prepared by the method of the present invention

[0039] Pacific oyster neutral polysaccharide finished product press figure 1 The process shown is prepared, and the specific steps are as follows:

[0040] (1) Preparation of defatted oyster powder: the dried oyster powder was crushed, passed through a 40-mesh sieve, and dried in an oven at 60°C until constant weight. Weigh 6g of oyster powder and add it to 60ml of deionized water, add 3 times the volume of acetone to wash, centrifuge at 6000rpm at 4°C for 20min, and dry at low temperature to obtain defatted oyster powder.

[0041] (2) Preparation of oyster polysaccharide crude extract: put the defatted oyster powder obtained in step (1) into a 1000mL glass extraction container, add 600mL deionized water and place it in a 500W ultrasonic microwave extractor for processing at a temperature of 86°C. Time 26min, cooling after extraction. The extract was...

Embodiment 2

[0058] Embodiment 2: HPLC detects the molecular weight and the purity of the Pacific oyster neutral polysaccharide prepared by the method of the present invention

[0059] Conditions: separation column: TSKgel G4000SW (300mm×7.5mm); mobile phase: ammonium acetate solution (0.05mol / L); sample concentration: detailed data later; injection volume: 20μL; flow rate: 0.5mL / min, drift tube temperature : 55°C, carrier gas flow rate: 2L / min, impactor: on. The result is shown in 6, which is a symmetrical peak type with a narrow peak width.

Embodiment 3

[0060] Example 3: Detection of monosaccharide composition determination in Pacific oyster neutral polysaccharide prepared by the method of the present invention (1) Weigh 10 mg of Pacific oyster neutral polysaccharide prepared by the method of the present invention, place it in a 10 mL graduated test tube with stopper, add 1 mol / L of H 2 SO 4 2mL, sealed. Hydrolyze at a constant temperature of 100°C for 4 hours, and after cooling the hydrolyzate, wash with saturated Ba(OH) 2 Neutralize to neutral, centrifuge at 3000rpm for 10min to remove BaSO 4 precipitation. Transfer all the supernatant to a 10mL measuring bottle, dilute to the mark with distilled water, and filter the solution through a 0.45 μm microporous membrane to obtain oyster neutral polysaccharide hydrolyzate.

[0061] (2) HPLC-ELSD instrument detection: glucose standard (500μg / mL), oyster neutral polysaccharide hydrolyzate, chromatographic column Carbohydrate ES 5u (250mm×4.6mm); mobile phase: acetonitrile-wate...

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Abstract

Disclosed in the present invention is a preparation method for a Pacific oyster neutral polysaccharide, comprising the following steps: (1) defatting Pacific oyster dry powder by means of acetone and drying the same to obtain defatted oyster powder; (2) dissolving the defatted oyster powder in water and treating the same using an ultrasonic microwave extractor, centrifuging a solution which is obtained after treatment, and then evaporating and concentrating a supernatant to obtain a crude oyster polysaccharide extract; (3) adding the crude oyster polysaccharide into an ethanol-(NH4)2SO4 dual aqueous phase system to perform extraction and isolation, an extracted lower phase being a crude liquid oyster neutral polysaccharide extract; (4) dialyzing the crude liquid oyster neutral polysaccharide extract to obtain a refined oyster polysaccharide liquid extract, wherein an oyster neutral polysaccharide may be obtained after concentration and drying. The present invention has a simple extraction process, is low cost, and is suitable for intermittent and large-scale production of high purity, high yield and high bio-activity oyster neutral polysaccharide products, while the prepared oyster neutral polysaccharide may be stored for a long period of time, has high purity, may increase the immune activity of mouse splenic lymphocytes and inhibit the activity of Hepg-2 liver cancer cells.

Description

technical field [0001] The invention relates to the field of deep processing of natural products, in particular to a Pacific oyster neutral polysaccharide and its extraction method and application. Background technique [0002] Oyster is the largest cultured shellfish in the world, and it is also an important marine shellfish in my country. It belongs to the Oyster family and is a bivalve mollusc. It is distributed in coastal waters of temperate and tropical oceans. The main species are near river oysters, Pacific oysters, and Dalian Bay oysters. and dense scale oysters. Pacific oysters have left and right shells connected by ligaments and adductor muscles. The left shell is concave, large and thick, and can be used to attach other objects. There are many radial ribs on the surface of the shell, which are clearly countable; the right shell is small and flat, with multiple concentric ring-shaped scales on the surface, without obvious radial ribs. [0003] Oyster is a kind of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00A61P37/02A61K31/716
CPCA61K31/716C08B37/0003C08B37/0009
Inventor 刘杨夏李轩张杰良肖湘钟名其伦镜盛
Owner SHANTOU UNIV
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