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Method for producing xylanase by anaerobic fermentation of corn straws

A technology of xylanase and anaerobic fermentation, which is applied in the field of anaerobic fermentation of corn stalks to produce xylanase, which can solve the problem of unsatisfactory effect of compound flora of artificial components, poor treatment effect of single microorganism, and long pretreatment time and other issues, to achieve important industrial application value and development prospects, high-efficiency lignocellulose degradation ability, and low equipment requirements

Active Publication Date: 2017-07-28
BAIYIN SINO BIOTECH
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  • Application Information

AI Technical Summary

Problems solved by technology

The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anaerobic digestion, but these pretreatment methods are costly and not environmentally friendly
Ordinary microbial treatment also has many defects. The effect of single microbial treatment is not good, and the effect of composite flora of artificial components is not ideal. There is mutual antagonism among various strains, resulting in long pretreatment time and low conversion efficiency. There is a well-established protocol for anaerobic fermentation of corn stover to produce xylanase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment one, Piromyces Yak TZ +M. ruminantium Preparation of bacteria.

[0029] Draw 1mL Piromyces Yak TZ +M. ruminantium The co-culture was inoculated into 9 mL of anaerobic medium based on air-dried and pulverized wheat straw in a 20 mL Heinz anaerobic tube, and compound antibiotics were added at the same time to make the final concentration of the anaerobic medium solution be Penicillin 1600 IU / mL and streptomycin sulfate 2000 IU / mL. After anaerobic culture at 39°C for 72 hours, the growth peak is reached, and the fermentation broth is a high-activity bacterial agent at this time.

Embodiment 2

[0030] Example 2: Production of xylanase by anaerobic fermentation of corn stalks.

[0031] 45 mL of liquid basic medium was placed in a 100 mL volume anaerobic fermentation bottle, and 0.5 g of crushed and air-dried corn stover was used as the substrate. Deoxygenation. Sterilize. Subcultured for 72 h Piromyces Yak TZ +M. ruminantium Use a sterile syringe to draw 5 mL of the co-culture and inoculate it into the above-mentioned anaerobic medium with corn stalks. At the same time, add compound antibiotics so that the final concentration in the anaerobic medium solution is 1600 IU / mL of penicillin and streptomycin sulfate. 2000 IU / mL, cultured anaerobically at 39 °C for 7 days.

[0032] The fermentation broth of the co-culture was 1000× g After centrifugation for 10 min, the supernatant was taken as the crude enzyme solution, and the xylanase activity was determined by the DNS method with a spectrophotometer. The specific method is as follows: diluted crude enzyme solution...

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PUM

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Abstract

The invention discloses a method for producing xylanase by anaerobic fermentation of corn straws. A fermentation strain used in the method comprises natural coexisting Piromyces Yak TZ+M. ruminantium coculture separated from rumen fluid of yak fully grazed in a Tianzhu Nanniwan pasture on the Tibetan plateau, and the collection number of the coculture at the China General Microbiological Culture Collection Center is CGMCC No. 12952. The coculture serving as the strain is used for anaerobic fermentation of the corn straws, the xylanase can be efficiently produced, the enzymatic activity of the xylanase generated by degrading the corn straws through the coculture within the culture period of 7 days reaches up to 6905mU, and the method is short in fermentation time, simple and convenient in fermentation process and has a wide application and development prospect.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a method for anaerobically fermenting corn stalks to produce xylanase. Background technique [0002] Corn is the main food crop in my country, with a wide sowing area, and the amount of straw produced every year is also very huge. At present, a large number of corn stalk resources in rural areas of our country are completely in the state of high consumption, high pollution, low utilization rate and low output, and corn stalks have not been rationally developed and utilized as an energy source. Corn stalks can be regenerated as resources through anaerobic digestion treatment, but the existing anaerobic digestion technology has the problems of low technical efficiency and great difficulty in popularization. [0003] Xylanase system is a kind of enzyme system that degrades xylan, which can degrade xylan-like hemicellulose that exists in large quantities in nature. Xyl...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12R1/645C12R1/01
CPCC12N9/248
Inventor 魏亚琴周剑平王治业
Owner BAIYIN SINO BIOTECH
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