Method for synthesizing monoglyceride in ionic liquid system by enzymic method

An ionic liquid and enzymatic synthesis technology, applied in fermentation and other fields, can solve the problems of low reaction selectivity, environmental pollution, and many side reactions, and achieve the effects of increasing contact area, wide liquid range, and improving mass transfer

Pending Publication Date: 2017-08-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, in the lipase catalyzed reaction, although the organic solvent as the reaction medium has the advantages of increasing the reaction rate, easing the reaction conditions and improving the stability of the enzyme, there are many side reactions, low reaction selectivity, and easy to produce toxic gas, which is easy to cause harm to the human body. Disadvantages such as polluting the environment

Method used

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  • Method for synthesizing monoglyceride in ionic liquid system by enzymic method
  • Method for synthesizing monoglyceride in ionic liquid system by enzymic method
  • Method for synthesizing monoglyceride in ionic liquid system by enzymic method

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Experimental program
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Effect test

Embodiment 1

[0029] 1.1 Screening of ionic liquids

[0030] Mix 1.049g oleic acid (5mmol) and 0.460g glycerin (5mmol), add ionic liquid (0.5608g) accounting for 30% of the total mass of the substrate as a reaction medium, and after stirring, add 4% of the total mass of the substrate. For lipase Novozyme435, react at a temperature of 60° C. for 8 hours with magnetic stirring, and then centrifuge to remove the ionic liquid (including glycerol) and lipase to obtain a product containing monoglyceride. The content of MAG in the reaction system was detected by normal phase high performance liquid chromatography with evaporative light detector (NP-HPLC-ELSD) and quantified by external standard method, and the change of fatty acid content was detected by acid-base titration. Esterification rate=(1-molar weight of fatty acid after reaction / molar weight of fatty acid before reaction)×100%

[0031] Analysis of MAG content by NP-HPLC-ELSD

[0032] Take out 20ul reaction mixture each time at a certai...

Embodiment 2

[0057] Add lauric acid and glycerol to the reactor with a molar ratio of 1:1, add 30% ionic liquid [HO(CH 2 ) 2 MIM]NTf 2 , put in a stirrer, preheat in a constant temperature water bath with a temperature of 60°C, turn on the magnetic stirrer, stir at a speed of 450rpm, add lipase Novozyme435 accounting for 4% of the total mass of the substrate, 4% 4A molecular sieve, and start After reacting for 8 hours, the product was taken out, and the ionic liquid and lipase were removed by centrifugation at 10,000 rpm for 10 minutes. According to liquid phase analysis, the content of monoglyceride in the product was 52.5%.

Embodiment 3

[0059] Caprylic acid and glycerin are added in the reactor with the molar ratio of 1:1, add the ionic liquid [MeO(CH 2 ) 2 MIM]NTf 2 , put in a stirrer, preheat in a constant temperature water bath with a temperature of 60°C, turn on the magnetic stirrer, stir at a speed of 450rpm, add lipase Novozyme435 accounting for 4% of the total mass of the substrate, 4% 4A molecular sieve, and start After reacting for 8 hours, the product was taken out, and the ionic liquid and lipase were removed by centrifugation at 10,000 rpm for 10 minutes. According to liquid phase analysis, the content of monoglyceride in the product was 51.0%.

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Abstract

The invention discloses a method for synthesizing monoglyceride in an ionic liquid system by an enzymic method. The method comprises the following steps: by taking an ionic liquid as a reaction medium, mixing glycerinum and fatty acid; by taking lipase as a catalyst and taking a molecular sieve as a dehydrant, performing stirring reaction; finally centrifugally separating to obtain a monoglyceride product. According to the method, the condition that ionic liquids are different in polarity is utilized to select the ironic liquid with greater polarity to selectively synthesize the monoglyceride, thus not only improving the output of the monoglyceride, but also effectively avoiding the defects that an organic solvent is volatile, poisonous gas easily hurts a human body and pollutes environment, and the like.

Description

technical field [0001] The invention belongs to the technical field of synthesizing structural glycerides, in particular to a method for enzymatically synthesizing monoglycerides in an ionic liquid system. Background technique [0002] Fatty acid glyceride (MAG) is a polyol type nonionic surfactant. Different carbon numbers of fatty acids make them have different hydrophilic-lipophilic balance (HLB), and have different uses in industry. In recent years, it is more and more inclined to use high-purity MAG in various industries, and the usage is also increasing year by year. According to the name of the constituent fatty acids, MAG can be divided into glyceryl monostearate, glyceryl monopalmitate, glyceryl monolaurate, glyceryl monooleate, etc. Among them, glyceryl monostearate is the most widely used . The synthesis methods of MAG include chemical catalysis and enzymatic catalysis. Chemical catalysis often needs to be operated at high temperature, causing serious problems...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62
CPCC12P7/62
Inventor 邹孝强吕松泰钱海峰秦杰黄磊金青哲王兴国
Owner JIANGNAN UNIV
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