Method for detecting components in liquid

A detection method and liquid technology, applied in the direction of measuring devices, analysis materials, material excitation analysis, etc., can solve the problems of complicated sample preparation process and inability to eliminate interference, and achieve the effect of saving experimental materials and low detection limit

Inactive Publication Date: 2017-08-01
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its detection limit can only reach 5×10 -4 M, and the interference of other monosaccharides on glucose detection cannot be ruled out
Non-patent literature (Volume 47, Issue 44October 20, 2008Pages 8386–8389) also discloses a method

Method used

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  • Method for detecting components in liquid
  • Method for detecting components in liquid
  • Method for detecting components in liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Take a piece of Au atom-bombarded track with an average density of 5×10 7 piece / cm 2 , a PET (polyethylene terephthalate) film with a thickness of 12 μm was irradiated under a 365-nm ultraviolet lamp for 30 minutes on the front and back, and then soaked in a 2M NaOH solution at 50 ° C for 5 minutes, and the Au atoms The bombarded sneak tracks were etched to a depth of 12 μm, a diameter of 30 nm, and a density of 5 × 10 7 / cm 2 A cylindrical channel that runs through the film, and after modifying p-aminophenylboronic acid in the nanopore, the surface of the channel has boric acid groups.

[0042] (2) Immerse this PET film in 4mM glucose solution, add 50 μ M TPEDB (two boronic acid groups modify tetraphenylethylene), the chemical structural formula of TPEDB is The transmembrane voltage after reaction at 25°C for 20 minutes is as follows: Figure 1a As shown (the specific conditions for voltage detection are: sandwich the PET film in the middle of the tank where e...

Embodiment 2

[0049] Repeat Example 1 with the same steps described, it is characterized in that, the concentration of glucose is respectively 4 * 10 -1 nM, 4nM, 4×10nM, 4×10 2 nM, 4×10 3 nM, 4×10 4 nM, 4×10 5 nM and 4×10 6 nM, the reaction time is 20min, the obtained concentration-current reduction rate detection curve is as follows Figure 2a As shown, the concentration-fluorescence detection curve is as Figure 2b It can be seen that in the case of the same reaction time, the higher the glucose concentration, the more the current decreases, and the greater the fluorescence value. Fluorescence detection can reach 4×10 -10 mol / L detection limit.

Embodiment 3

[0051] Repeat embodiment 1 with described identical steps, it is characterized in that, get supernatant after urine sample (taking patient's urine sample 1mL earlier at the rotating speed of 800rap / s for 3min, supernatant is again in 12000rap / s) s speed of centrifugation for 20min and then take the supernatant) to replace the glucose solution, the current reduction curve obtained after the reaction for 20min is as follows image 3 As shown, ND is non-diabetic urine sample, DM (before) is the urine sample of diabetic patient before treatment, and DM (after) is the urine sample of diabetic patient after treatment. It can be seen that the urine sample of diabetic patient after treatment and glucose concentrations in non-diabetic urine samples were significantly higher than in pre-treatment urine samples from diabetic patients.

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Abstract

The invention discloses a method for detecting components in liquid, which comprises the flowing steps of adding an aggregation-induced emission compound and a nanochannel membrane into liquid to be detected, enabling the aggregation-induced emission compound to be fully polymerized with the component to be detected in a nanochannel, and obtaining the concentration of the component to be detected according to the change of an electrochemical signal and/or a fluorescence signal of the nanochannel membrane; wherein the nanochannel membrane contains a surface group capable of being combined with the aggregation-induced emission compound or the component to be detected, the aggregation-induced emission compound contains at least two probe groups, and the probe groups are used for being combined with target groups or electronic vacant orbits of the component to be detected. According to the method provided by the invention, the aggregation-induced emission compound is used as a detection probe for the nanochannel membrane, not only is the technical defect that the component to be detected must match with DNA (deoxyribonucleic acid) to amplify the electrochemical signal overcome, but also the change of the fluorescence signal is introduced into the nanochannel membrane detecting technique, so that the detection is more accurate.

Description

technical field [0001] The invention belongs to the field of trace detection, and more specifically relates to a detection method for components in liquid. Background technique [0002] Nanochannel technology is a detection technology with low energy consumption, simple operation, high sensitivity and no pollution, which can be applied to DNA sequencing research and detection of small molecules. In the prior art, capture probes on nanochannels are usually used to detect small molecules such as cysteine ​​(Adv Mater. Volume 26, Issue 3, January 22, 2014Pages 455-460.) Since this method only adsorbs a A layer of cysteine ​​molecules and a layer of capture probe molecules, so the detection sensitivity is limited. [0003] Using DNA as a capture probe, due to the larger molecular weight of DNA, can increase the detection sensitivity to 10 -6 M, however, because this method requires that the DNA and the components to be tested can be complementary to each other, it can only det...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N21/64
CPCG01N27/26G01N21/6428
Inventor 夏帆徐雪梅娄筱叮
Owner HUAZHONG UNIV OF SCI & TECH
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