Extraction method for thalli produced in amino acid fermentation

An extraction method and fermentation bacteria technology, which are applied in the field of amino acid fermentation bacteria extraction, can solve the problems of not fully excavating application potential, unsuitable for production enterprises, low industrial added value, etc., and achieve simple and feasible extraction methods, wall breaking rate and high The degree of hydrolysis is complete and the effect of increasing industrial added value

Pending Publication Date: 2017-08-04
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the bacteria are prepared as feed additives, which can achieve certain economic benefits, but they are low-end products, and a small number of manufacturers apply the bacteria to fertilizer preparation.
The applicant's previous patented technology "A Method for Utilizing Threonine Cellular Protein" processed bacterial protein into protein feed, which achieved certain economic benefits, but the industrial added value was relatively low, and its application has not been fully explored. Potential; the applicant's previous patented technology "Using Amino Acid Fermentation Waste Bacteria to Prepare Liquid Organic Fertilizer" has prepared organic fertilizer, which can be implemented in enterprises with fertilizer production capacity, but it is not suitable for all production enterprises
The applicant's previous patented technology "A Method for Utilizing Amino Acid Fermentation Bacteria" is specifically a method for enzymatically hydrolyzing glutamic acid fermentation thallus using compound enzyme preparations to prepare enzymatic protein paste and protein powder. The industrial added value of body protein maximizes the profit of the enterprise, but it uses a large amount of enzyme preparations, which increases the cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting amino acid fermentation cells, which comprises the following steps:

[0024] Step 1) Drying and crushing: place the amino acid fermented bacteria at 80°C to dry, and then pulverize them into bacterial powder;

[0025] Step 2) Salt treatment and ultrasonic treatment: Add the same mass of sodium chloride aqueous solution with a concentration of 7wt% to the bacterial powder, adjust the pH to 3 with sulfuric acid, then heat to 60°C, and use ultrasonic treatment under heat preservation conditions. The processing time is 20min; the ultrasonic frequency is 20KHz, and the power is 1000W;

[0026] Step 3) Hydrolysis: Put the material obtained in step 2) in a reactor, control the pressure in the reactor to 0.8MPa, the temperature to 100°C, keep the heat and pressure for 8 minutes, then reduce the pressure to normal pressure, and maintain the temperature at 100°C, then Sulfuric acid was added to adjust the pH to 1, and the hydrolysis time was 3 hours to ob...

Embodiment 2

[0029] A method for extracting amino acid fermentation cells, which comprises the following steps:

[0030] Step 1) Drying and crushing: place the amino acid fermented bacteria at 80°C to dry, and then pulverize them into bacterial powder;

[0031] Step 2) Salt treatment and ultrasonic treatment: Add the same mass of sodium chloride aqueous solution with a concentration of 5-7wt% to the bacterial powder, adjust the pH to 2 with sulfuric acid, then heat to 60°C, and use ultrasonic waves under heat preservation conditions. Processing, processing time is 30min; ultrasonic frequency is 20KHz, power is 1000W;

[0032] Step 3) Hydrolysis: Put the material obtained in step 2) in a reactor, control the pressure in the reactor to 1.0MPa, the temperature to 105°C, keep the pressure for 6 minutes, then reduce the pressure to normal pressure, and maintain the temperature at 100°C, then Sulfuric acid was added to adjust the pH to 1, and the hydrolysis time was 4 hours to obtain a hydrolyz...

Embodiment 3

[0035] A method for extracting amino acid fermentation cells, which comprises the following steps:

[0036] Step 1) Drying and crushing: place the amino acid fermented bacteria at 80°C to dry, and then pulverize them into bacterial powder;

[0037] Step 2) Salt treatment and ultrasonic treatment: Add the same mass of sodium chloride aqueous solution with a concentration of 5-7wt% to the bacterial powder, adjust the pH to 2.5 with sulfuric acid, then heat to 65°C, and use ultrasonic waves under heat preservation conditions. Processing, processing time is 25min; ultrasonic frequency is 20KHz, power is 1000W;

[0038] Step 3) Hydrolysis: Put the material obtained in step 2) in the reactor, control the pressure in the reactor to 0.9MPa, the temperature to 102°C, keep the heat and pressure for 7 minutes, then reduce the pressure to normal pressure, and maintain the temperature at 100°C, then Sulfuric acid was added to adjust the pH to 1, and the hydrolysis time was 4 hours to obta...

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PUM

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Abstract

The invention discloses an extraction method for thalli produced in amino acid fermentation, belonging to the technical field of production of amino acids. The extraction method comprises the following steps: step 1) drying and crushing; step 2) salt treatment and ultrasonic treatment; step 3) hydrolysis; and step 4) evaporation and spray granulation. To reduce cost for raw materials, the extraction method does not use expensive enzyme preparations for treatment; and thallus protein powder with comprehensive nutrient composition is prepared, and the thallus protein powder can be used for replacing yeast extract and has better fermentation and culture effect.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a method for extracting amino acid fermentation cells. Background technique [0002] Fermentation of amino acids will produce a large amount of waste bacteria. By measuring the nutritional composition of the bacterial protein, it is found that the amino acid fermented bacterial protein is rich in protein, nucleic acid, carbohydrates, vitamins and other nutrients. Taking the glutamic acid bacteria produced by fermentation of MSG as an example, it is a single-cell protein rich in protein. The analysis of the chemical composition of the dried bacterial protein found that the protein content in the glutamic acid bacteria was as high as 85.8 % The total amino acid content is 78.77%, which is higher than soybean meal and yeast, which are commonly used raw materials for protein hydrolyzate. [0003] At present, most of the bacteria are prepared as feed additiv...

Claims

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Application Information

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IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 王峰唐永强汲广习沈兆胜胡俊礼
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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