Lactic acid bacteria with formaldehyde degradation function and application thereof
A formaldehyde degradation, lactic acid bacteria technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of low efficiency of formaldehyde degradation, achieve long degradation time, good effect, good scientific significance and application value Effect
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Embodiment 1
[0025] Example 1: Screening, isolation and identification of strains
[0026] (1) Screening and isolation of strains
[0027] Put a small amount of sauerkraut juice sample in 20mL sterile physiological saline, add glass beads to shake for 10min, then let it stand still, take 1mL in the basic medium, then add a certain amount of 0.1g / L formaldehyde, shake at 220r / min, 37℃ cultivated in. Take 200 μL of the culture solution and spread it on the basic medium plate with formaldehyde as the only carbon source, culture the culture dish upside down in a 37 °C incubator, observe the colony morphology and pick a single colony on the culture dish for further identification.
[0028] (2) Strain identification
[0029] Morphological characteristics of the colony: the colony is round, medium in size, raised, slightly white, moist, with neat edges, a diameter of 3 mm ± 1 mm, the back of the colony is yellow, and the cells are often arranged in chains.
[0030] Microscopic examination of G...
Embodiment 2
[0032] Embodiment 2: strain formaldehyde resistance test
[0033] Add 1g / L, 2g / L, 3g / L, 4g / L, and 5g / L formaldehyde to the basic medium, inoculate 500μL of seed solution, and measure the OD 600 Observe the growth of bacteria. It was found that the basic medium OD containing more than 4g / L formaldehyde 600 is 0, indicating that the strain cannot grow under this formaldehyde concentration. Reconstituted with 3.1g / L, 3.2g / L, 3.3g / L, 3.4, g / L, 3.5g / L, 3.6g / L, 3.7g / L, 3.8g / L, 3.9g / L formaldehyde The basic medium was also inoculated with 500 μL of seed liquid, and the growth of bacteria was observed. It was found that the strains could not grow in the basic medium containing more than 3.8g / L formaldehyde. Thus, it was determined that the strain's maximum tolerance to formaldehyde concentration was 3.8g / L.
Embodiment 3
[0034] Embodiment 3: Indoor degradation formaldehyde test
[0035] Conditions: humidity 45%, temperature 25°C, volume 55m 3 , more than 7.5kg of lactic acid bacteria culture solution was repeatedly sprayed 3 times in 5 rooms. The sampling volume is 10mL, and the test is carried out with reference to the indoor air quality standard GB / T18883-2002. The results show that the product has the effect of promoting the release of formaldehyde at the beginning, and the product is still working after 10 days, and the amount of formaldehyde release is gradually decreasing. The daily formaldehyde value has dropped below the initial test value, 30 days is close to the national regulation range, and 35 days all rooms are below the national standard. After one, two, and three years of follow-up tests, it has been proved that this product is continuously stable and effective.
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