A kind of preparation method of highly active sugarcane anthocyanins
A kind of anthocyanin and high activity technology, applied in the preparation of sugar derivatives, chemical instruments and methods, organic chemistry, etc., can solve the problems of low extraction efficiency, poor antioxidant activity, etc., achieve rapid separation, convenient operation, and improve utilization rate Effect
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Embodiment 1
[0035]1. Collect the bagasse just after squeezing the juice from the sugar cane sugar factory, dry it and crush it, take 500g of bagasse powder, add 5L of distilled water, and adjust the pH to 4.0 with buffer. Compound enzyme is prepared; the compound enzyme is composed of cellulase, hemicellulase and pectinase, wherein the mass ratio of cellulase, hemicellulase and pectinase is 5:3:1. Mix the above compound enzymes, sterilize, add to the extraction tank, the addition amount is 8mg / kg bagasse, stir and extract at 35°C for 2h, inactivate the extract for 10min, centrifuge at 3000r / min, and concentrate in vacuo to obtain Sugarcane anthocyanin crude extract A 1 .
[0036] 2. The macroporous resin AB-8 and dextran gel Sephadex LH-20 are packed into a column after pretreatment, and the concentrated solution obtained is diluted to 4.2g / L by using a glass chromatography column (7.5mm×240mm). 1000mL of diluent is loaded into the sample, and the flow rate of the sample is controlled a...
Embodiment 2
[0040] 1. Collect the bagasse just after squeezing the juice from the sugar cane sugar factory, dry it and crush it, take 750g of bagasse powder, add 7.5L of distilled water, and adjust the pH to 4.0 with buffer. Compound enzyme is prepared; the compound enzyme is composed of cellulase, hemicellulase and pectinase, wherein the mass ratio of cellulase, hemicellulase and pectinase is 16:8:3. Mix the above compound enzymes, sterilize them, add them to the extraction tank, the addition amount is 8 mg / kg bagasse, stir and extract at 40°C for 4 hours, inactivate the enzymes in the extracts for 10 minutes, centrifuge at 3000r / min, and concentrate in vacuo to obtain Sugarcane anthocyanin crude extract B 1 .
[0041] 2. The macroporous resin D101 and dextran gel Sephadex G-25 are pretreated and then packed into a column. Use a glass chromatography column (7.5mm×240mm) to dilute the obtained shrinkage solution to 6.4g / L, and take 800mL for dilution The liquid is loaded, and the flow r...
Embodiment 3
[0045] 1. Collect the bagasse just after squeezing the juice from the sugarcane sugar factory, dry it and crush it, take 839g of bagasse powder, add 8.39L of distilled water, and adjust the pH to 4.0 with buffer. Compound enzyme is prepared; the compound enzyme is composed of cellulase, hemicellulase and pectinase, wherein the mass ratio of cellulase, hemicellulase and pectinase is 3:2:1. Mix the above complex enzymes, sterilize them, add them to the extraction tank, the addition amount is 14 mg / kg bagasse, stir and extract at 30°C for 5 hours, inactivate the enzymes in the extracts for 10 minutes, centrifuge at 3000r / min, and concentrate in vacuo to obtain Sugarcane anthocyanin crude extract C 1 .
[0046] 2. The macroporous resin NKA-9 and dextran gel Sephadex G-50 are packed into columns after pretreatment, and the concentrated solution obtained is diluted to 5.2g / L by using a glass chromatography column (7.5mm×240mm). 900mL of diluent is loaded into the sample, and the f...
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