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Application of MBL in preparing drug for preventing or treating disease taking Tregs as target spot

A disease, drug technology, applied in the field of new uses of mannan-binding lectin, to achieve the effect of enriching the source of umbilical cord

Pending Publication Date: 2017-08-18
XINXIANG MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether there are still unknown factors that promote the induction and differentiation of Tregs cells needs to be further developed and studied urgently

Method used

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  • Application of MBL in preparing drug for preventing or treating disease taking Tregs as target spot
  • Application of MBL in preparing drug for preventing or treating disease taking Tregs as target spot
  • Application of MBL in preparing drug for preventing or treating disease taking Tregs as target spot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Experimental Materials

[0030] 1. The source of cells used in the experiment

[0031] Neonatal umbilical cord blood was collected from the Third Affiliated Hospital of Xinxiang Medical College and the Department of Obstetrics and Gynecology of Xinxiang First People's Hospital of full-term healthy newborns. Immediately after the fetus was delivered, blood was collected from the umbilical vein of the placenta and placed in a blood collection bag containing 28 mL of preservation solution. All blood samples were delivered to the laboratory within 4 hours after sampling.

[0032] 2. Main reagents

[0033]Medium RPMI1640, fetal bovine serum (FBS), and 10×PBS solution were all purchased from Thermo Fisher Scientific.

[0034] Lymphocyte separation medium (Ficoll-Paque PLUS), which is a sterile endotoxin (<0.12 EU / mL) test solution of FicollTMPM400 and sodium diatrizoate with a density of 1.077g / mL, was purchased from GE Healthcare Life Sciences.

[0035] Anti-human CD3 mon...

Embodiment 2

[0118] Experimental Materials

[0119] 1. The source of cells used in the experiment

[0120] Reference example 1

[0121] 2. Main reagents

[0122] Reference example 1

[0123] 3. Main instruments and equipment

[0124] Reference example 1

[0125] 4. Preparation of common reagents

[0126] Reference example 1

[0127] experimental method

[0128] 1. Preparation of umbilical cord blood mononuclear cells

[0129] Reference example 1

[0130] 2. Coated with anti-CD3mAb

[0131] Reference example 1

[0132] 3. Magnetic separation of CD4+CD25-T cells and CD4+CD25+T cells

[0133] sample preparation

[0134] Peripheral mononuclear cells were separated by density gradient centrifugation. To remove platelets, cells were resuspended in buffer and centrifuged at 200×g for 10-15 min. The supernatant was carefully aspirated and washed repeatedly.

[0135] Magnetic labeling of non-CD4+ T cells

[0136] The whole process should be completed quickly, and the cells should be k...

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Abstract

The invention discloses an application of MBL in preparing a drug for preventing or treating disease taking Tregs as a target spot and belongs to the technical field of a novel application of mannan binding lectin. The key point of the technical scheme is as follows: the invention relates to the application of MBL in preparing a drug for preventing or treating infectious diseases, autoimmune diseases or / and allergic diseases induced by taking Tregs as the target spot, wherein MBL realizes functions of the drug by accelerating induced differentiation of a CD4<+>CD25-T cell toward a Tregs cell. Through exploration, a novel factor of adjusting induced differentiation of the Tregs cell is obtained, that is, MBL directly enhances induced differentiation of the CD4<+>CD25-T cell toward the Tregs cell. The application disclosed by the invention highlights a novel function of MBL, and MBL not only plays an important anti-infection role in inherent immunity, but also can be a pertinent target spot for treating various autoimmune diseases and inflammatory diseases in intervention of induced differentiation of the Tregs cell.

Description

technical field [0001] The invention relates to the technical field of new applications of mannan-binding lectins, in particular to the application of MBL in the preparation of drugs for the prevention or treatment of diseases targeting Tregs. Background technique [0002] Mannose-binding lectin (mannan-binding lectin, MBL) is a highly conserved plasma protein mainly secreted by hepatocytes, and is a member of the collectins family in the C-type lectin superfamily. MBL is also an acute phase response protein, and its plasma concentration can increase 2-3 times during stress (such as pathogen infection, surgical operation, etc.). MBL, known as ante-antibody, widely recognizes sugar structures distributed on the surface of various pathogens such as bacteria, viruses, parasites, fungi, etc., including D-mannose, through its carbohydrate recognition domain (CRD) Sugar, L-fucose, N-acetylglucosamine, N-acetylmannosamine, etc. Its collagen-like region (collagen-like region, CLR)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P31/00A61P37/02A61P37/08A61P33/00A61P11/02A61P11/06A61P3/10A61P29/00A61P19/02A61P17/00A61P17/14A61P5/14
CPCA61K38/1732Y02A50/30
Inventor 王明永王凡平康丽霞张卫斌王闪闪李克君陈晨李俊鹏吴敏娜段巨洪
Owner XINXIANG MEDICAL UNIV
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