Application of MBL in preparing drug for preventing or treating disease taking Tregs as target spot
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A disease, drug technology, applied in the field of new uses of mannan-binding lectin, to achieve the effect of enriching the source of umbilical cord
Pending Publication Date: 2017-08-18
XINXIANG MEDICAL UNIV +1
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Problems solved by technology
However, whether there are still unknown factors that promote the induction and differentiation of Tregs cells needs to be further developed and studied urgently
Method used
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Embodiment 1
[0029] Experimental Materials
[0030] 1. The source of cells used in the experiment
[0031] Neonatal umbilical cord blood was collected from the Third Affiliated Hospital of Xinxiang Medical College and the Department of Obstetrics and Gynecology of Xinxiang First People's Hospital of full-term healthy newborns. Immediately after the fetus was delivered, blood was collected from the umbilical vein of the placenta and placed in a blood collection bag containing 28 mL of preservation solution. All blood samples were delivered to the laboratory within 4 hours after sampling.
[0032] 2. Main reagents
[0033]Medium RPMI1640, fetal bovine serum (FBS), and 10×PBS solution were all purchased from Thermo Fisher Scientific.
[0034] Lymphocyte separation medium (Ficoll-Paque PLUS), which is a sterile endotoxin (<0.12 EU / mL) test solution of FicollTMPM400 and sodium diatrizoate with a density of 1.077g / mL, was purchased from GE Healthcare Life Sciences.
[0035] Anti-human CD3 mon...
Embodiment 2
[0118] Experimental Materials
[0119] 1. The source of cells used in the experiment
[0120] Reference example 1
[0121] 2. Main reagents
[0122] Reference example 1
[0123] 3. Main instruments and equipment
[0124] Reference example 1
[0125] 4. Preparation of common reagents
[0126] Reference example 1
[0127] experimental method
[0128] 1. Preparation of umbilical cord blood mononuclear cells
[0129] Reference example 1
[0130] 2. Coated with anti-CD3mAb
[0131] Reference example 1
[0132] 3. Magnetic separation of CD4+CD25-T cells and CD4+CD25+T cells
[0133] sample preparation
[0134] Peripheral mononuclear cells were separated by density gradient centrifugation. To remove platelets, cells were resuspended in buffer and centrifuged at 200×g for 10-15 min. The supernatant was carefully aspirated and washed repeatedly.
[0135] Magnetic labeling of non-CD4+ T cells
[0136] The whole process should be completed quickly, and the cells should be k...
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Abstract
The invention discloses an application of MBL in preparing a drug for preventing or treating disease taking Tregs as a target spot and belongs to the technical field of a novel application of mannan binding lectin. The key point of the technical scheme is as follows: the invention relates to the application of MBL in preparing a drug for preventing or treating infectious diseases, autoimmune diseases or/and allergic diseases induced by taking Tregs as the target spot, wherein MBL realizes functions of the drug by accelerating induced differentiation of a CD4<+>CD25-T cell toward a Tregs cell. Through exploration, a novel factor of adjusting induced differentiation of the Tregs cell is obtained, that is, MBL directly enhances induced differentiation of the CD4<+>CD25-T cell toward the Tregs cell. The application disclosed by the invention highlights a novel function of MBL, and MBL not only plays an important anti-infection role in inherent immunity, but also can be a pertinent target spot for treating various autoimmune diseases and inflammatory diseases in intervention of induced differentiation of the Tregs cell.
Description
technical field [0001] The invention relates to the technical field of new applications of mannan-binding lectins, in particular to the application of MBL in the preparation of drugs for the prevention or treatment of diseases targeting Tregs. Background technique [0002] Mannose-binding lectin (mannan-binding lectin, MBL) is a highly conserved plasma protein mainly secreted by hepatocytes, and is a member of the collectins family in the C-type lectin superfamily. MBL is also an acute phase response protein, and its plasma concentration can increase 2-3 times during stress (such as pathogen infection, surgical operation, etc.). MBL, known as ante-antibody, widely recognizes sugar structures distributed on the surface of various pathogens such as bacteria, viruses, parasites, fungi, etc., including D-mannose, through its carbohydrate recognition domain (CRD) Sugar, L-fucose, N-acetylglucosamine, N-acetylmannosamine, etc. Its collagen-like region (collagen-like region, CLR)...
Claims
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