In vitro fertilization liquid of porcine oocytes added with traditional Chinese medicinal monomer and cultivation method

Abstract

The invention discloses an in vitro fertilization liquid of porcine oocytes added with a traditional Chinese medicinal monomer. The in vitro fertilization liquid is characterized by comprising a conventional culture solution of the porcine oocytes and a traditional Chinese medicinal monomer, wherein the traditional Chinese medicinal monomer is any one of hyperoside, icariin and ligustrazine; and the mass ratio / volume ratio range of the traditional Chinese medicinal monomer to the conventional culture solution of the porcine oocytes is 2mu g / ml, 0.5mu g / ml and 0.5mu g / ml. The invention further provides a method for improving ectogenesis of in vitro fertilized embryos of pigs on the basis of the fertilization liquid. The research shows that after the traditional Chinese medicinal monomer hyperoside is added to the in vitro fertilization liquid, the in vitro fertilization liquid has a good promotion effect on embryonic development of the porcine oocytes, and is better than a control group free of a traditional Chinese medicinal ingredient in the aspects of the cleavage rate and the blastocyst development rate.

Description

technical field [0001] The invention specifically relates to a porcine oocyte in vitro fertilization fluid added with traditional Chinese medicine monomers. Background technique [0002] Organ transplantation is one of the effective means to save patients with end-stage organ diseases, but the development of organ transplantation is greatly restricted by the severe shortage of donors. With the development of biotechnology and the consensus on ethics, seeking cells, tissues, and organs needed by patients from animals to achieve xenotransplantation has become a research hotspot in the field of medicine today. Because the physiological and anatomical indicators of pigs are very similar to those of humans, they also have the advantages of short breeding period, large number of litters, rapid growth of piglets, easy feeding under designated pathogen-free (DPF) conditions, and ethical comparison with primates. Due to the characteristics of less medical barriers, transgenic pigs h...

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Problems solved by technology

At the same time, this also caused difficulties in embryo cryopreservation.

Some progress has been made in promoting the maturation of oocytes by adding hormones, cytokines or growth factors to the culture medium, but the developmental ability of oocytes matured in vitro is still inferior to that of oocytes matured in vivo, which shows that the oocytes mature in vitro The mature system is not yet perfect and needs further optimization

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