Peste des petits ruminants virus H-F fusion protein, biological material related to same and application of peste des petits ruminants virus H-F fusion protein

Abstract

The invention discloses a peste des petits ruminants virus H-F fusion protein, a biological material related to the same and application of the peste des petits ruminants virus H-F fusion protein. The peste des petits ruminants virus H-F fusion protein is a protein a) or a protein b). The protein a) comprises amino acid sequences shown as SEQ ID No.2; the protein b) is a soluble protein, and each amino acid sequence shown as SEQ ID No.2 is substituted and / or deleted and / or added by an amino acid residue or a plurality of amino acid residues to obtain the soluble protein. The peste des petits ruminants virus H-F fusion protein, the biological material and the application have the advantages that indirect ELISA (enzyme-linked immunosorbent assay) detection methods for peste des petits ruminants virus antibodies are created by the aid of the peste des petits ruminants virus H-F fusion protein which is used as an envelope antigen, are high in specificity and sensitivity and good in accuracy and can be quickly, easily and conveniently implemented, and accordingly the peste des petits ruminants virus H-F fusion protein, the biological material and the application are favorable for clinically monitoring peste des petits ruminants.

Description

technical field [0001] The invention relates to the H-F fusion protein of Peste des Petits Ruminants virus and its related biological materials and application in the field of biotechnology. Background technique [0002] Peste des Petits Ruminants (PPR) is an acute, febrile, contagious disease caused by Peste des Petits Ruminants Virus (PPRV), which is characterized by high morbidity and mortality. At present, there is no effective method to treat Peste des petits ruminants. The only methods to control the disease are vaccination, culling after the outbreak, and strengthening regular serum monitoring. The most common reports at home and abroad use purified PPRV N and H proteins (full length) or partially recombinant N and H protein fragments containing the main antigenic epitopes of N protein as coating antigens, and there are also concentrated PPRV whole viruses as packaging antigens. An indirect ELISA method was established to detect PPR serum antibodies by the antigen (Q...

AI Technical Summary

Problems solved by technology

However, the expression products of Peste des petits ruminants H and F proteins expressed in most studies usually exist in the form of inclusion bodies of insoluble monovalent proteins, and the expression of proteins with soluble H-F fusion activity has not been reported

The expression of monovalent inclusion body protein cannot form high-level protein structure and natural spatial conformation epitope due to insufficient expression and misfolding of spatial structure, resulting in that it cannot form a good immune spatial epitope as a diagnostic antigen

At the same time, the expression product in the monovalent inclusion body has no biological activity, so it needs to be denatured and refolded

Protein denaturation and renaturation is an extremely complicated process. The renaturation conditions of different proteins are different, and the renaturation rate is often difficult to improve.

This is the main constraint limiting its application

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