Building method based on parameter-adjustable-and-controllable self assembly structure substrate and SERS detection method for narcotic drugs

A detection method and self-assembly technology, applied in the field of SERS detection, can solve the problems of serious coffee ring effect, commercialization restrictions, and high detection cost, and achieve the effects of simple and practical method steps, wide application range and high sensitivity

Inactive Publication Date: 2017-08-29
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Common drug detection methods are generally expensive, time-consuming, and require professional personnel. The emergence of SERS technology can make up for the shortcomings of the above methods
The more widely used method of drug detection is to drop the prepared metal sol substrate on the silicon wafer, and then drop the analyte on the substrate. This traditional method has an obvious problem in the actual operation process. The disadvantage is that the coffee ring effect of the substrate is relatively serious during detection, and the reproducibility of the detection process cannot be achieved well, which limits our practical application and commercialization.

Method used

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  • Building method based on parameter-adjustable-and-controllable self assembly structure substrate and SERS detection method for narcotic drugs
  • Building method based on parameter-adjustable-and-controllable self assembly structure substrate and SERS detection method for narcotic drugs
  • Building method based on parameter-adjustable-and-controllable self assembly structure substrate and SERS detection method for narcotic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Soak the silicon wafer in aqua regia and fresh aqua regia successively, wash it with a large amount of ultrapure water, and dry it with nitrogen for later use;

[0052] (2) After cleaning the synthesized gold nanorods with ultrapure water centrifugation (7500r) twice, redisperse them in ultrapure water, and the dispersion concentration is 60wt% gold nanorods solution;

[0053] (3) Add the gold nanorod solution dropwise to the five silicon wafers treated in step (1), the volumes of which are 1 μL, 2 μL, 4 μL, 6 μL, and 8 μL, and turn the silicon wafers upside down after the addition Put it in a simulated climate box for the laboratory, and use the solvent evaporation mechanism to induce the gold nanorod sol to form a gold nanofilm on the silicon wafer, which is the SERS substrate;

[0054] in: figure 1 Optical scanning electron microscopy (SEM) of gold nanofilms at different ratios for the SERS substrate formed by adding a volume of 1 μL gold nanorod solution, from ...

Embodiment 2

[0059] (1) After the silicon chip is soaked in aqua regia and fresh aqua regia and processed successively, after washing with a large amount of ultrapure water, dry it with nitrogen to obtain a clean silicon chip;

[0060] (2) After washing the synthesized gold nanorods with ultrapure water centrifugation (7500r) twice, redisperse them in ultrapure water, and disperse into mass concentrations of 20%, 40%, 60%, 80%, and 100% respectively. % five groups of gold nanorod solutions;

[0061] (3) Drop the five groups of gold nanorod solutions onto the silicon wafers treated in step (1) respectively, with a volume of 4 μL. After the addition, place the silicon wafers upside down in a simulated climate box for the laboratory. Evaporation induced film formation, which is the SERS substrate;

[0062] (4) Take the human body fluid of taking drugs, add organic solvent alcohols or ketones to extract, then centrifuge the extract, and take its organic layer solution; the amount of organic s...

Embodiment 3

[0065] (1) Soak the silicon wafers in aqua regia and fresh aqua regia in sequence, wash them with a large amount of ultrapure water, and dry them with nitrogen for later use;

[0066] (2) After cleaning the synthesized gold nanorods with ultrapure water centrifugation (7500r) twice, redisperse them in ultrapure water, and the dispersion concentration is 60wt% gold nanorods solution;

[0067] (3) Drop the gold nanorod solution onto the silicon wafer treated in step (1) with a volume of 4 μL. After the addition, place the silicon wafer at an angle of 45 degrees in a simulated climate box in the laboratory to evaporate Induced film formation, which is the SERS substrate;

[0068] (4) Take the human urine of the drug ecstasy, add organic solvent alcohols or ketones to extract, then centrifuge the extract, and get its organic layer solution; wherein the amount of organic solvent added is 0.8mL per 0.8mL of human urine Add 0.3 mL.

[0069] (5) Drop the organic layer solution onto ...

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Abstract

The invention provides a building method based on a parameter-adjustable-and-controllable self assembly structure substrate and an SERS detection method for narcotic drugs. The method comprises the following steps of after synthesized gold nanometer rod sol is subjected to centrifugal cleaning and concentration, dispersing the gold nanometer rod sol into ultrapure water again to form a gold nanometer rod solution with the mass concentration being 10 to 90 percent; dripping the gold nanometer rod sol onto a treated silicon water; tipsily installing the silicon wafer into a simulated climate box for a laboratory; inducing the gold nanometer rod sol to perform self assembly on the substrate by using a solvent evaporation mechanism to obtain an SERS substrate; then, taking the body fluid of people taking the narcotic drugs; performing centrifugation on the pretreated extraction liquid of the body fluid; then, taking an organic layer solution; dripping the organic layer solution onto the SERS substrate; performing SERS detection by using a portable raman spectrometer. The method has the advantages that the uniform SERS substrate is formed through self assembly, so that the SERS detection on the narcotic drug molecules can be sensitive and practical; the detection reproducibility is realized.

Description

technical field [0001] The invention belongs to the technical field of SERS detection, and in particular relates to a method for constructing a self-assembled structural substrate based on adjustable parameters and a SERS detection method for drugs. Background technique [0002] According to the 2016 "World Drug Report" published by the United Nations Office on Drugs and Crime: the global situation of drug use is still serious, with more than 247 million people involved in illegal drug use. The drugs popular in the world mainly include opiates (heroin and opium), amphetamine-type stimulants (methamphetamine and ecstasy, etc.), marijuana and so on. And now there is a new class of psychoactive substances, substances of abuse in pure or formulated form that are not regulated by the international drug conventions but may pose a risk to public health. Among them, the seizure, production and consumption of amphetamine-type stimulants are increasing year by year, and they are the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B82Y40/00
CPCB82Y40/00G01N21/658
Inventor 杨良保陈诚唐祥虎毛妹于博荣孟娟曹晓敏
Owner 安徽中科赛飞尔科技有限公司
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