Method for preparing cerium oxide nano-particle functional membrane layer-loaded pure titanium implant material
A nanoparticle and cerium oxide technology, applied in coatings, prostheses, pharmaceutical formulations, etc., has good clinical application prospects, promotes osseointegration, prevents and repairs the failure rate of implants
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Embodiment 1
[0025] Example 1 Preparation of CNPs-loaded implant material of the present invention and its property detection
[0026] 1. Preparation method
[0027] (1) Preparation of CNPs (Cerium Oxide Nanoparticles)
[0028] At room temperature, add 5mL of 0.2mol / L cerium nitrate solution into 10mL of 0.2mol / L dimethyl oxalate solution, add 0.05g-1g of carbon black at the same time, and stir evenly. This solution was heated to 60°C and reacted for 20h. The obtained product is filtered, washed, dried, placed in a crucible, and calcined at 700°C for 2 hours in an air atmosphere to obtain CeO 2 Nanopowder (i.e. cerium oxide nanoparticles).
[0029] The obtained nano-cerium oxide particles such as figure 1 shown.
[0030] (2) Preparation of pure titanium samples loaded with CNPs functional film layer
[0031] Using commercial medical TA2 pure titanium, process it into Φ=14mm, 1mm thick disc sample (in vitro experiment) and Φ3.75mm*8mm columnar implant (in vivo experiment), and perform...
experiment example 1
[0041] Experimental example 1 Restoration effect of the implant material loaded with CNPs of the present invention
[0042] To establish the diabetic rabbit model, 6-month-old male New Zealand rabbits were anesthetized by intravenous injection of pentobarbital sodium, and then slowly injected with alloxan. A few hours after injection, subcutaneously inject 50g / L glucose solution to prevent hypoglycemia, and provide 100g / L glucose drinking water within 24 hours. Blood glucose was detected every 24 hours during the modeling period. After modeling, they were fed normally for 6 weeks, and their blood sugar and body weight were measured every week. The modeling was successful when the fasting blood glucose was stable above 13.9mmol / L.
[0043] Control group:
[0044] Control group 1: medical TA2 pure titanium, columnar implant of Φ4mm*8mm
[0045] Control group 2: medical TA2 pure titanium after MAO treatment, Φ4mm*8mm columnar implant (in vivo experiment), and MAO treatment to...
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