Method for reducing abundance of tetracycline resistance genes in organic fertilizer

Abstract

The invention relates to a method for reducing the abundance of tetracycline resistance genes in an organic fertilizer. The method comprises the following steps: performing pyrolysis on a bamboo at 600 DEG C to obtain biochar; then by taking wheat straws in chicken manure compost as a conditioner, uniformly mixing the air-dried chicken manure with the chopped wheat straws, and regulating the initial C / N ratio to 25:1; adding the biochar accounting for 2.5% of the mixture based on dry weight into the mixture, and adding distilled water to regulate the water content to 60%; and after the composted materials are sufficiently and uniformly mixed, performing composting in a full-automatic composting device, wherein oxygen introduction is ensured by a forced ventilation process. After 26 days of composting, tetracycline resistance genes (tetG, tetW and tetX) are reduced by 70% or above by the treatment of the added biochar, and the removal rate is obviously higher than that of the control treatment. According to the invention, the method reduces the abundance of tetracycline resistance genes in chicken manure compost; and meanwhile, the method has the advantages of short composting period, simple equipment, easy operation, low cost, high compost product stability and safety and the like.

Description

technical field [0001] The invention belongs to the technical field of aquaculture solid waste treatment, in particular to a method for reducing the abundance of tetracycline resistance genes in organic fertilizers. Background technique [0002] Because antibiotics can promote animal growth and prevent diseases, antibiotics are widely used in intensive livestock and poultry farming. Antibiotics can directly induce the resistance of microorganisms in animals (such as intestinal bacteria), which are directly excreted with animal feces. The organic fertilizer made from the livestock and poultry manure carrying the resistance gene will have adverse effects on the soil, water and other environments after being applied. Recent studies have detected the presence of resistance genes in both soil and vegetables. Resistance genes can spread among microorganisms through horizontal gene transfer (conjugation, transformation, and transduction). Once these resistance genes are transferr...

AI Technical Summary

Problems solved by technology

In recent years, studies have shown that composting can remove resistance genes in livestock and poultry manure, but traditional composting has problems such as long composting cycle, high content of heavy metals in the effective state of composting products, and low fertilizer efficiency.

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