Coculture and method using coculture to ferment wheat straw to produce methane

A technology of co-culture and wheat straw, applied in the field of biotechnology renewable energy, can solve the problems of limited rumen fluid source, long pretreatment time, inconvenient use, etc., and achieve high efficiency lignocellulose degradation ability, breakthrough time and The effect of geographical restriction and improving the efficiency of anaerobic fermentation

Active Publication Date: 2017-09-05
GANSU ACAD OF SCI INST OF BIOLOGY
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anaerobic digestion, but these pretreatment methods are costly and not environmentally friendly
Ordinary microbial treatment also has many defects. The effect of single microbial treatment is not good, and the effect of composite bacterial flora of artificial components is not ideal. There is mutual antagonism between various bacterial species, resulting in long pretreatment time and low conversion efficiency.
[0004] Patent CN 105505995 A This patent proposes a method of using rumen microorganisms to pretreat rice straw to increase methane production, which has achieved certain results. However, this method uses rumen fluid for pretreatment, and the source of rumen fluid is limited. It must be after slaughtering ruminants. secondly, the components in the rumen fluid are complex, and the treatment effect is not stable; thirdly, the directly taken rumen fluid must be used immediately, which is inconvenient for storage and transportation, and the scope of application is limited; finally, the components in the rumen fluid are diverse, and the smell is not good. easy to use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Coculture and method using coculture to ferment wheat straw to produce methane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment one, Piromyces Yak TZ +M. ruminantium Preparation of bacteria.

[0029] Draw 1mL Piromyces Yak TZ +M. ruminantium The co-culture was inoculated into 9 mL of anaerobic medium based on air-dried and crushed wheat straw in a 20 mL Heinz anaerobic tube, and compound antibiotics were added at the same time. After adding the compound antibiotics, the final concentration of penicillin in the medium solution was: 1600 IU / mL and streptomycin sulfate: 2000 IU / mL, anaerobic culture at 39°C for 72 hours, the growth peak will be reached, and the fermentation broth is a highly active bacterial agent at this time.

Embodiment 2

[0030] Embodiment 2, anaerobic fermentation of straw to produce methane.

[0031] 45 mL of liquid basic medium was placed in a 100 mL volume anaerobic fermentation bottle, and 0.5 g of air-dried and crushed wheat straw was used as a substrate, deoxygenated and sterilized. Subcultured for 72h Piromyces Yak TZ +M. ruminantium Use a sterile syringe to draw 5 mL of the co-culture and inoculate it into the above-mentioned anaerobic medium with wheat straw, and add compound antibiotics at the same time (final concentration of penicillin in the medium solution: 1600 IU / mL and streptomycin sulfate: 2000 IU / mL ), cultured anaerobically at 39°C for 7 days. A total of 3 parallel experiments were set up, and the amount of methane in the anaerobic bottle was measured every 24 h. Determination of methane by gas chromatography: use a gas chromatograph (GC522, Wufeng Instruments, China), equipped with a GPS101 column (2 m × 3 mm), thermal conductivity detector, vaporization chamber 250 ° ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses anaerobic fungus-methanogens coculture and a method using the coculture to ferment wheat straw to produce methane. The coculture is naturally-coexisting PiromycesYak TZ+M. ruminantium separated from the rumen fluid of fully-grazing yak of the Gansu Tianzhu Nanniwan pasture of the Qinghai-Tibet Plateau, and the preservation number of the coculture in the China General Microbiological Culture Collection Center is CGMCC No. 12952. When the coculture is used to perform anaerobic fermentation on the wheat straw, a large amount of methane can be produced, the quantity of the methane produced after the coculture degrades the wheat straw for 7 days reaches 3.7mmol/g DM, fermentation time is short, the fermentation process is simple, and the coculture is promising in application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a co-culture of anaerobic fungus methane bacteria and a method for anaerobically fermenting wheat straw to produce methane. Background technique [0002] Wheat is the main food crop in my country, with a wide sowing area, and the amount of straw produced every year is also very huge. In recent years, on a global scale, with the increase of the population and the improvement of the quality of life, the demand for energy has risen sharply. Traditional energy materials are in danger of being depleted, and the use of these traditional energy materials produces a large amount of greenhouse gases and pollutes the environment. Wheat straw has received more and more attention as a renewable energy source. But at present, a large number of wheat straw resources in rural areas of our country are completely in the state of high consumption, high pollution, low utilization rat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12R1/01C12R1/645
CPCC12P5/023Y02E50/30
Inventor 魏亚琴周剑平王治业
Owner GANSU ACAD OF SCI INST OF BIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap