Coculture and method using coculture to ferment wheat straw to produce methane

Abstract

The invention discloses anaerobic fungus-methanogens coculture and a method using the coculture to ferment wheat straw to produce methane. The coculture is naturally-coexisting PiromycesYak TZ+M. ruminantium separated from the rumen fluid of fully-grazing yak of the Gansu Tianzhu Nanniwan pasture of the Qinghai-Tibet Plateau, and the preservation number of the coculture in the China General Microbiological Culture Collection Center is CGMCC No. 12952. When the coculture is used to perform anaerobic fermentation on the wheat straw, a large amount of methane can be produced, the quantity of the methane produced after the coculture degrades the wheat straw for 7 days reaches 3.7mmol / g DM, fermentation time is short, the fermentation process is simple, and the coculture is promising in application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a co-culture of anaerobic fungus methane bacteria and a method for anaerobically fermenting wheat straw to produce methane. Background technique [0002] Wheat is the main food crop in my country, with a wide sowing area, and the amount of straw produced every year is also very huge. In recent years, on a global scale, with the increase of the population and the improvement of the quality of life, the demand for energy has risen sharply. Traditional energy materials are in danger of being depleted, and the use of these traditional energy materials produces a large amount of greenhouse gases and pollutes the environment. Wheat straw has received more and more attention as a renewable energy source. But at present, a large number of wheat straw resources in rural areas of our country are completely in the state of high consumption, high pollution, low utilization rat...

AI Technical Summary

Problems solved by technology

The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anaerobic digestion, but these pretreatment methods are costly and not environmentally friendly

Ordinary microbial treatment also has many defects. The effect of single microbial treatment is not good, and the effect of composite bacterial flora of artificial components is not ideal. There is mutual antagonism between various bacterial species, resulting in long pretreatment time and low conversion efficiency.

[0004] Patent CN 105505995 A This patent proposes a method of using rumen microorganisms to pretreat rice straw to increase methane production, which has achieved certain results. However, this method uses rumen fluid for pretreatment, and the source of rumen fluid is limited. It must be after slaughtering ruminants. secondly, the components in the rumen fluid are complex, and the treatment effect is not stable; thirdly, the directly taken rumen fluid must be used immediately, which is inconvenient for storage and transportation, and the scope of application is limited; finally, the components in the rumen fluid are diverse, and the smell is not good. easy to use

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