A γ-decalactone-producing Dicrotophorus strain and its application
A technology of decanolactone and microporia, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., to achieve high application value, abundant raw material sources, and low production costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] Example 1 Isolation and Purification of the Trichomonas Strains Producing γ-Decalactone
[0040] The strains were collected from the fruiting bodies of basidiomycetes in the wild. Cut the fresh fruiting body with a sterile scalpel, tear off a little bacterial flesh with ophthalmic tweezers, inoculate it on a PDA plate, and culture it at 30°C. When the diameter of the mycelium mat reaches 2-3 cm, pick a little mycelium from the edge of the mycelium and inoculate it on a new PDA plate, and transfer it repeatedly until the pure strain is obtained. The target strain was re-screened by shake flask fermentation and further isolated and screened to obtain a stable γ-decalactone producing strain, which was named Piptoporus sp. A-9.
[0041] The above-mentioned strain Trichomona A-9 has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on May 25, 2017. The preservation address is: No. 3, Yard 1, Beichen West R...
Embodiment 2
[0045] Example 2 Sequencing of the 18S rDNA and ITS sequence of the Trichozoa A-9 CGMCC No.13895 strain
[0046] The γ-decalactone-producing strain isolated and purified in Example 1, that is, the CGMCC No.1869 strain, was entrusted to Beshang Biological Company to perform 18S rDNA sequencing.
[0047] The experimental method is: pick the slant culture, extract the genome as a template, use the universal primers NS1 and NS8 to amplify the 18srRNA gene, the fragment size is about 1.8kb, and use the universal primers ITS1 and ITS4 to amplify the ITS sequence, the fragment size is about 750bp .
[0048] Take 5 μl for agarose gel electrophoresis, use TaKaRa Agarose Gel DNA Purification Kit Ver.2.0 (Code No. DV805A) to cut the gel and recover the target fragment, and perform DNA sequencing on the recovered product.
[0049] Sequencing results:
[0050] The 18S rDNA sequence length of CGMCC No.13895 strain is 1679bp, and its nucleotide sequence is shown in SEQ ID NO.1; the ITS seq...
Embodiment 3
[0054] Example 3 Application of Trichropore A-9 CGMCC No.13895 in the preparation of gamma-decalactone by shake flask culture
[0055] The sequence of steps involved in applying the method is as follows:
[0056] (1) Strain selection: select Piptoporus sp. A-9 CGMCC No.13895;
[0057] (2) slant culture activation: inoculate the strains on the slant medium, and culture it statically for 100 hours under the condition of 30° C., and set aside;
[0058] (3) Seed culture: the bacterial strain cultivated in step (2) is put into 90-100mL (500mL Erlenmeyer flask) liquid seed medium with an inoculation loop under aseptic conditions, and the rotation speed is 160 rpm. Minutes, on a shaker with a rotation radius of 40mm, culture at 30°C for 80 hours to obtain a seed solution;
[0059] (4) Shake flask fermentation: with the inoculum amount of 8-10% volume ratio, inoculate the seed liquid in the shake flask that 90-100mL (500mL Erlenmeyer flask) fermentation medium is housed, 30-35 ℃, ro...
PUM
| Property | Measurement | Unit |
|---|---|---|
| thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More