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Extraction method for ferulic acid in rice bran meal

An extraction method, ferulic acid technology, which is applied in the field of ferulic acid extraction with a purity ≥ 98%, can solve the problems of high product purification cost and difficult industrial application, and achieve the goals of reducing repeated use costs, expanding sources, and reducing environmental pollution Effect

Active Publication Date: 2017-09-22
XIAN HEALTHFUL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process does not use immobilized enzymes, but uses complex enzymes, so that the colloids and furfural in the rice bran enter the extract, resulting in high purification costs and difficult industrial applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Immobilized ferulic acid esterase

[0031] Disperse 5 mg of ferulic acid esterase (50 U / mg) in 1 mL of 0.1 mol / L sodium acetate buffer solution with pH=5.5, then add 5 mg of magnetosomes, place on a shaker at 150 rpm for 30 minutes at room temperature, and then add 0.05 mL100U / mL transglutaminase-Tris-HCl solution, continue shaking reaction at room temperature for 4 hours, after the reaction, use magnet adsorption to separate, and the solid product is washed three times with sodium acetate buffer solution to obtain immobilized ferulic acid esterase. The specific activity of enzyme is 22U / mg.

[0032] 2. Pretreatment of rice bran meal

[0033] Add 1 g of rice bran meal (with a ferulic acid content of 5 mg / g) after extracting rice bran oil into 20 mL of deionized water, and add 0.02 g of immobilized ferulic acid esterase, and enzymolyze it at 30 ° C for 60 minutes under ultrasonic conditions, wherein The frequency of ultrasound is 25Hz and the power is 60W.

[0034]...

Embodiment 2

[0041] In step 2 of the pretreated rice bran meal in this embodiment, add 1 g of rice bran meal (with a ferulic acid content of 5 mg / g) after extracting rice bran oil into 21 mL of deionized water, and add 0.02 g of immobilized ferulic acid esterase, Enzymolysis was performed at 30° C. for 60 minutes under ultrasonic conditions, wherein the ultrasonic frequency was 50 Hz and the power was 60 W. The other steps were the same as in Example 1 to obtain ferulic acid with a purity ≥ 98%, and the yield of ferulic acid was 83%.

Embodiment 3

[0043]In step 1 of the immobilized ferulic acid esterase of the present embodiment, 5 mg of ferulic acid esterase (50 U / mg) was dispersed in 1 mL of 0.1 mol / L sodium acetate buffer solution with pH=5.5, and then 2.5 mg of magnetic Place the body in a shaker at 150rpm for 30 minutes at room temperature, then add 0.05mL 100U / mL transglutaminase-Tris-HCl solution, and continue to shake at room temperature for 4 hours. After the reaction, use a magnet to absorb and separate the solid product. The buffer solution was washed three times to obtain the immobilized ferulic acid esterase, wherein the specific activity of the immobilized enzyme was 18 U / mg. The other steps were the same as in Example 1 to obtain ferulic acid with a purity ≥ 98%, and the yield of ferulic acid was 85.2%.

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Abstract

The invention discloses an extraction method for ferulic acid in rice bran meal. According to the method, cross-linking fixation is carried out on biomagnetic nanometer materials such as magnetosome and feruloyl esterase by adopting transglutaminase so as to obtain immobilized feruloyl esterase, then ferulic acid ester in rice bran meal after rice bran oil is extracted is pretreated by adopting the immobilized feruloyl esterase, extraction is carried out by using isopropyl alcohol after pretreatment, after solid-liquid separation of the extracting solution, then microfiltration and nanofiltration two-stage decontamination and low-temperature vacuum concentration are adopted, and activated carbon decoloration and recrystallization carried out on concentrated solution, so that a ferulic acid product with the purity of more than 98% is obtained. Natural ferulic acid is extracted from rice bran meal by adopting the immobilized feruloyl esterase, so that the problems of environmental pollution, low safety and high industrialized production cost existing in the prior art are overcome, and the extraction method has preferable application prospects.

Description

technical field [0001] The invention belongs to the technical field of extracting ferulic acid, and in particular relates to a method for extracting ferulic acid with a purity of ≥98% from rice bran meal after extracting rice bran oil. Background technique [0002] Sodium ferulate is widely found in Chinese herbal medicines such as Ferulicum, Angelica, Chuan Shao, Cimicifuga, Semen Jujuba, and food materials such as coffee, chaff, vanilla beans, wheat drums, and rice bran. It has anti-platelet aggregation, inhibits platelet The formation of thromboxane, the enhancement of prostaglandin activity, analgesia, and the relief of vasospasm are the basic raw materials for the production of drugs for the treatment of cardiovascular and cerebrovascular diseases and leukopenia. Studies in recent years have found that many ferulic acid and its derivatives have pharmacological effects and biological activities such as anti-inflammatory and anti-aging, and have low toxicity, so they are ...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12N11/14C07C51/42C07C51/43C07C59/64
CPCC07C51/42C07C51/43C12N9/18C12N11/14C12P7/42C12Y301/01073C07C59/64
Inventor 高永华李玉松孟利军
Owner XIAN HEALTHFUL BIOTECH
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