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Microparticle chroma clustering analysis method and kit

A technology of cluster analysis and microparticles, applied in the field of biomedicine, can solve problems such as difficult cost reduction, interference, and high cost performance, and achieve low cost, low cost, and good detection sensitivity

Active Publication Date: 2017-09-22
胡胜勤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the discs of this technology adopt microfabrication technology, the cost is difficult to reduce, and the discs are easy to form stacks in the detection system, causing large interference
[0012] It can be seen that people have been exploring multiple detection technology, and multiple detection technology has been improving, but there is still no reliable, cost-effective technology that can simultaneously realize multiple detection of a single sample and rapid multiple detection of multiple specimens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Identification of Common Unexpected Antibodies in Human Blood Types by Microparticle Chromatic Cluster Analysis

[0057] Human blood group accidental antibody refers to the immune antibody produced by the body against the blood group antigen or antigen subtype that it does not have after blood transfusion and pregnancy. May cause hemolytic disease of the newborn in the fetus. Therefore, accidental antibody screening and identification of unexpected antibodies are important tests for blood transfusion and pregnancy testing. At present, accidental antibody screening and accidental antibody identification are two detection items. First, the antibody screening reagent cells are used to react with the specimen to be tested. If there is agglutination, it means that there is an unexpected antibody, and if there is no agglutination, it means that there is no unexpected antibody; In this case, it is necessary to use a group of antibodies to identify the reagent cells to react w...

Embodiment 2

[0072] Detection of tumor markers by microparticle chromaticity cluster analysis

[0073] Tumor is a common and frequently-occurring disease, which seriously endangers the life safety of patients. Laboratory diagnosis is an important method to detect tumors early and win treatment opportunities for patients. Due to the diversity of tumors, it is often necessary to conduct joint detection of dozens of tumor markers at the same time in order to find tumors with a broad spectrum. In this embodiment, the high-efficiency detection of tumor markers is realized by utilizing the multi-analyte detection capability of the microparticle chromaticity cluster analysis method and the high-throughput detection capability of specimens. Since dozens of tumor markers have been discovered, for ease of expression, this example only uses the most common alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), neuron-specific enolase (NSE) Detection of seven tumor markers, including squamous cell...

Embodiment 3

[0088] Infection Screening of Blood Donors Using Microparticle Chromatic Cluster Analysis

[0089] In order to avoid blood transfusion-transmitted diseases, it is an international practice in the blood collection and supply industry to screen blood donor samples for pathogens transmitted by blood. The mandatory screening items stipulated by Chinese laws and regulations include HIV-1 antibody, HIV-p24, HBsAg, HCV antibody and TP antibody Blood banks often need to repeat the above screening for dozens or even hundreds of blood donors at the same time. In this embodiment, the multi-analyte detection capability and the high-throughput detection capability of specimens of the microparticle chromaticity cluster analysis method are used to realize infection screening of blood donors. For the convenience of expression, in this embodiment, the HIV-GP41 antibody is used as the representative of the HIV-1 antibody, the HCV-NS3 antibody is used as the representative of the HCV antibody, a...

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Abstract

The invention discloses a microparticle chroma clustering analysis method and a kit. The method comprises the following steps: S1, creating a biological target detection agent which can be subjected to specific binding reaction with M different biological targets in a sample to be detected; S2, performing biological detection reaction to form a color microparticle-biological probe I-biological target to be detected-separating agent composite; S3, separating a biological detection reaction product, and separating out a color microparticle-biological probe I polymer without being subjected to the binding reaction with the detection agent; S4, determining the detection result, and counting the variety and the content of the biological targets to be detected in the detecting sample according to the one-to-one correspondence built between the color microparticle chroma and the biological target variety. The invention provides the kit for the method. With the adoption of the method, multi-detection of one sample and quick multi-detection of a plurality of samples can be achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a microparticle chromaticity cluster analysis method and a kit. Background technique [0002] Biomacromolecules, including proteins, nucleic acids, etc., embody or record information about life and disease, and are extremely important analysis objects in biological research and disease diagnosis. Detecting their existence and content is the basic work of scientific research and disease diagnosis In addition to the detection of biomacromolecules, in some occasions in disease diagnosis and biological research, it is also necessary to detect viruses and cells. These biomolecules, virus particles and cells are called detection biological targets in detection experiments. biotargets, DBTs). [0003] If DBTs are biomacromolecules, they are also called target molecules (TMs). For the detection of a single biomolecule (TM) to be tested, there are generally two technical...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/58
CPCG01N33/54313G01N33/585
Inventor 蒋天伦
Owner 胡胜勤
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