Method for carrying out tumor detection by utilizing graphene quantum dot
A graphene quantum dot and tumor technology, which can be applied to preparations for in vivo experiments, pharmaceutical formulations, etc., can solve problems such as inability to widely use tumor detection, limited tumor types, and complicated material preparation, and achieve good tumor IFP response effect. , Simple method, high targeting efficiency
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Embodiment 1
[0023] The ability of GQDs to respond to intratumoral pressure:
[0024] To test the specific targeting ability of pressure-responsive GQDs to tumors, breast cancer tumors were inoculated into the right hind limbs of mice by subcutaneous transplantation to establish animal tumor models. GQDs (30-200 mg / kg) were intravenously injected into the animal model every other day. After 0.5 h, the intratumoral pressure was detected, and the distribution of GQDs in the tumor tissue was observed by sectioning. The "needle core" technique was used to detect the IFP value in the tumor. Fill a standard 23-gauge injection needle with nylon thread and saline (to which 50 IE / ml heparin is added), insert it into the center of the tumor tissue, and connect the syringe to the pressure transducer. This device can continuously and stably detect fluid pressure. During detection, fluid connectivity between the needle and sensor is determined through compression and decompression. The average value...
Embodiment 2
[0026] GQDs tumor pressure response is sensitive, and after drug depressurization, tumor cell nuclei cannot be labeled:
[0027]Nicotinic acid was dissolved in normal saline, and niacin was injected intraperitoneally (500 mg / kg, 0.01 ml / g, detected 1 h after injection); after this method, IFP decreased by 60%. Imatinib was dissolved in normal saline, and imatinib was injected by intragastric administration (50 mg / kg, administered daily, tested after four days). The "needle and core" technique described above was used to detect the IFP value in the tumor. The average value of two readings was taken as the tumor IFP value, and the IFP value in the muscle or subcutaneous tissue was used as a blank. The tumor IFP value decreased by 40% in this method. Then 60 mg / kg GQDs were injected into the animal model for 0.5 h, and the tumor tissue was taken out to make a 20 μm thick cryosection sample, which was used to observe the distribution of GQDs in the tumor tissue by laser confocal ...
Embodiment 3
[0029] GQDs are non-toxic in vivo and in vitro, and can be rapidly and completely metabolized:
[0030] In the process of stress-sensitive response to targeted markers in tumor cell nuclei, none of the GQDs produced toxicity and could rapidly metabolize cells and tissues. After 0.5 h of tail vein injection, the tumor tissue was specifically targeted in vivo, and the nuclei of tumor tissue were efficiently labeled, and most of them were metabolized from the body after 24 h, showing low toxicity in vivo. A large number of GQDs are accumulated in the tumor tissue cells, only a small amount exists in the normal tissue interstitium, and do not enter the normal tissue nucleus. This method has no tumor specificity, and is universal and applicable.
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