Method for identifying lipid component structure in lipidomics based on CID fragmentation

A technology of lipid composition and lipidome, applied in the field of life sciences, which can solve problems such as difficulties and bottlenecks
CN107228908AInactive Publication Date: 2017-10-03NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Publication Date
2017-10-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a simple method for identifying unknown lipid components in lipidomics based on CID fragmentation. The main technical route comprises: 1, establishing a lipidomics analysis and determination method; 2, establishing the lipidomics analysis and determination method by using a multi-stage fragmentation mass spectrometry hybrid high resolution mass spectrometer; 3, searching an online spectrum library according to the accurate mass number, and attributing the lipid component types corresponding to the lipid chromatographic peaks to be identified; 4, determining the total carbon content and the unsaturation degree of the fatty acyl part in the lipid molecule; 5, according to a simple formula, determining the characteristic fragment information of each chromatographic peak; 6, deriving the carbon number and the unsaturation degree of each fatty acyl group according to the obtained characteristic fragment information; and 7, finally determining the lipid molecule structure. According to the present invention, the method is accurate and sensitive, is independent of the standard compound, and can rapidly identify the structure of the unknown lipid molecule in the lipidomics data.
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Description

Technical field:

[0001] The invention relates to life sciences, in particular to the establishment of metabolomics and lipidomics analysis and determination methods and the rapid identification of unknown lipid component structures. Background technique

[0002] Due to the integrity and complexity of living organisms, the development of modern biology has returned to a new level of integrative research from organs, tissues, cells to genes, which means that the era of molecular biology has entered the era of systems biology. Therefore, systems biology research methods such as genomics, transcriptomics, proteomics and metabolomics have become new means to interpret the mysteries of life sciences and clarify the mechanism of physiological and pathological changes and drug intervention.

[0003] Lipidomics is a system biological analysis method that has developed rapidly in recent years. It is a new omics method for studying biological systems developed after metabolomics and pr...

Claims

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