A kind of aggregation-induced luminescent antibacterial polypeptide probe and its preparation and application

Abstract

The invention belongs to the technical field of biomedical materials and discloses an aggregation-induced emission anti-microbial polypeptide probe, a preparation and an application. The aggregation-induced emission anti-microbial polypeptide probe is formed in the manner of connecting an aggregation-induced emission compound with an anti-microbial polypeptide. The aggregation-induced emission anti-microbial polypeptide probe is high in anti-microbial activity, can kill bacteria within short time and is difficult to generate drug resistance, and meanwhile, the aggregation luminous efficiency is high, the signal to noise ratio for bacteria imaging is high and the aggregation-induced emission anti-microbial polypeptide probe is used for monitoring the bacteria-killing combining mode and dynamic process of the anti-microbial polypeptide in real time.

Description

technical field [0001] The invention relates to a preparation method of an aggregation-induced luminescent antibacterial polypeptide probe and its application in sterilization, in particular to the application in monitoring the binding mode and binding kinetic process of antibacterial polypeptide and bacteria through the change of fluorescent signal. Background technique [0002] Bone defects or bone loss caused by trauma or disease are very common clinically. Traditional clinical treatment is mainly through autologous bone grafting or implanting biocompatible implant materials. However, despite preoperative antibiotic prophylaxis and material aseptic treatment of patients, the implant failure rate due to bacterial infection is still high in the early stages of implant surgery. According to clinical statistics, the effective rate of implants caused by bacterial infection is about 4%-6%. Such a high rate has attracted widespread attention internationally. The consequences o...

AI Technical Summary

Problems solved by technology

Existing fluorescence imaging mainly uses small molecule organic dyes such as fluorescein, but the fluorescence of traditional small molecule organic dyes has the defect of aggregation-induced quenching. When staining bacteria at a high density, the fluorescence will undergo obvious self-quenching. At the same time, its photostability is poor, and it is difficult to monitor the binding process of antibacterial peptides and bacteria and the way of sterilization in real time.

Images