Bacillus subtilis and application thereof

A technology of Bacillus subtilis and Lactobacillus plantarum, applied in application, bacteria, fungi and other directions, can solve problems such as waste and pollution of resources, and achieve the effects of improving fertility, reducing pests and diseases, and shortening fermentation time.

Active Publication Date: 2017-10-17
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Failure to properly handle these organic wastes w

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0028] The invention provides a preparation method of a composite microorganism decomposing inoculum, the method comprising:

[0029] (1) Bacillus subtilis is Bacillus subtilis zzl-002 whose preservation number is CGMCC No.13758, and its fermentation method is 10% glucose, 10% soybean meal cake, 10% corn flour, 6% calcium carbonate, 7% sodium chloride , and the rest is water. The culture temperature was 37°C, and the rotation speed was 210rpm. After fermentation, it is dried into powder by spray drying method.

[0030] (2) The spore content of each microorganism in the composite microbial decomposing inoculum is: ≥100 billion / gram of Bacillus subtilis; ≥20 billion / gram of Lactobacillus plantarum; ≥10 billion / gram of Trichoderma viride; ≥10 billion / gram g of Aspergillus niger; ≥ 20 billion / g of Saccharomyces cerevisiae.

[0031] (3) The four bacterial agents are Lactobacillus plantarum powder ≥ 20 billion / g; Trichoderma viride powder ≥ 10 billion / g; Aspergillus niger powder ...

Embodiment 1

[0035] Preparation of microbial decomposing inoculum

[0036] Bacillus subtilis is Bacillus subtilis zzl-002 with a preservation number of 13758, and its fermentation method is 10% glucose, 10% soybean meal cake, 10% corn flour, 6% calcium carbonate, 7% sodium chloride, and the rest is water. The culture temperature was 37°C, and the rotation speed was 210rpm.

[0037] Fermentation was stopped when the spore content was greater than or equal to 100 billion / g.

[0038] After fermentation, it is dried into powder by spray drying method.

[0039] A fungus powder with a spore content of ≥100 billion / g is obtained.

[0040] In this example, Lactobacillus plantarum ≥ 20 billion / g; Trichoderma viride ≥ 10 billion / g; Aspergillus niger ≥ 10 billion / g; Saccharomyces cerevisiae ≥ 20 billion / g Provided by Technology Co., Ltd.

[0041] The five kinds of powder microbial inoculants are mixed evenly by weight ratio of 40% of Bacillus subtilis, 20% of Lactobacillus plantarum, 10% of Trich...

Embodiment 2

[0043] Use the compound inoculum in Example 1 to decompose the straw.

[0044] In Changle, Weifang, Shandong, July.

[0045] The straw and chicken manure are mixed in a ratio of 20:1, and the mixture is one ton. One kilogram of inoculum in Example 1 was mixed with one ton of the above-mentioned mixture. as experiment 1.

[0046] The straw and chicken manure are mixed in a ratio of 20:1, and the mixture is one ton. Do not mix with inoculants. as comparison.

[0047] The two kinds of mixed materials are piled into cones with a height of about 1.5 meters and a bottom diameter of about 5 meters.

[0048] The temperature of the experimental group was earlier and faster than that of the control group, and the fermentation time was shortened by 34%.

[0049]

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Abstract

The invention discloses a bacillus subtilis zzl-002, preserved in the general microbiological center of Chinese microorganism preservation management committee (CGMCC for short, address: No.3, courtyard 1, Beichen road west, Chaoyang district of Beijing) in March 17, 2017, and the preservation number is CGMCC No.13758. The compound microorganism decayed bacterium is capable of shortening the fermentation time. The decayed organic fertilizer is capable of improving the fertility and reducing the plant diseases and insect pests.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of organic garbage. Background technique [0002] A large amount of plant straws and organic wastes are produced every year in agricultural production and some industrial production. Failure to properly handle these organic wastes will result in serious pollution and waste of resources. If these organic wastes are rapidly degraded and fully decomposed, a large number of excellent organic fertilizer products can be produced, thereby reducing the use of chemical fertilizers, increasing soil vitality, and improving soil. Therefore, there is an urgent need for an effective bacterial agent that can quickly degrade and fully decompose various organic wastes. SUMMARY OF THE INVENTION [0003] The first object of the present invention is to provide a strain of Bacillus subtilis (Bacillus subtilis) zzl-002, which has been preserved in the General Microorganism Center of the China Microorg...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/18C05F15/00C05F11/08C12R1/125C12R1/25C12R1/885C12R1/685C12R1/865
CPCC05F3/00C05F11/08C12N1/14C12N1/18C12N1/20C12N1/205C12R2001/125C05F11/00C05F17/00
Inventor 仲乃琴刘宁赵盼钞亚鹏赵东
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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