A bionic bone tissue engineering scaffold and its preparation method and application

A tissue engineering scaffold, bionic bone technology, used in tissue regeneration, pharmaceutical formulations, prostheses, etc.

Active Publication Date: 2020-08-07
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although various strategies for modifying scaffolds for bone tissue engineering have been developed, developing the closest to ideal technology remains a challenge

Method used

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  • A bionic bone tissue engineering scaffold and its preparation method and application
  • A bionic bone tissue engineering scaffold and its preparation method and application
  • A bionic bone tissue engineering scaffold and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1 Preparation of Biomimetic Bone Tissue Engineering Scaffold AG-o-CNTs

[0085] The preparation process of the bionic bone tissue engineering scaffold of the present invention is as follows: figure 1 As shown, first, carboxylated multi-walled carbon nanotubes (COOH-CNTs) are modified by dopamine to form dopamine-coated multi-walled carbon nanotubes (Dopamine-CNTs, D-CNTs). Afterwards, place this type of carbon nanotubes in simulated body fluid for 4-8 weeks, and the phosphorus ions and calcium ions in the simulated body fluid will form hydroxyapatite under the guidance of phenolic hydroxyl groups on dopamine. Walled carbon nanotubes (Hydroxyapatite-CNTS, H-CNTs, that is, multi-walled carbon nanotubes loaded with hydroxyapatite). Afterwards, the H-CNTs were formed into an ordered parallel array in the agarose gel by electrophoresis, thereby constructing a biomimetic ordered agarose-carbon nanotube scaffold (AG-o-CNTs scaffold).

[0086] Specifically, the prepa...

Embodiment 2

[0105] Example 2 Characterization of AG-o-CNTs Biomimetic Bone Tissue Engineering Scaffold

[0106] 1. Infrared spectrum detection

[0107] (1) Dry each sample and KBr in a dryer, mix 1-2 mg of sample with 200 mg of pure KBr and grind them evenly, and grind the mixture to a particle size of less than 2 μm to avoid the influence of scattered light. Put the mixture in the mold, press the mixture into a transparent sheet with a pressure of 5-10MPa on the hydraulic press, and wait for the machine to be determined; at the same time, dry the blank bracket and the modified bracket, and test it on the machine.

[0108] (2) Infrared detection of CNTs after dopamine and hydroxyapatite modification

[0109] To further determine the composition of the structures generated on the surface of sh-CNTs, we first analyzed the samples by Fourier transform infrared spectroscopy detection.

[0110] experiment as figure 2 As shown in Figure A, the results show that the infrared characteristic p...

Embodiment 3

[0137] Example 3 Application of bionic bone tissue engineering scaffold AG-o-CNTs

[0138] To study the effect of AG-o-CNTs biomimetic scaffold in promoting the growth of MSCs, and use the carbon nanotube modified agarose scaffold (AG-CNTs scaffold) without electrophoresis as a control.

[0139] 1. Cell culture

[0140] Place blank and modified scaffolds 1×1×1cm in 24-well cell culture plates respectively 3 , the cells were cultured to 60-90% confluency in the 4 -3×10 4 The density of / well was seeded into 24-well plates and cultured for 2, 4, and 6 days for subsequent experiments. Other cell culture conditions were: low-glucose DMEM medium containing 10% newborn calf serum, 37°C, 5.0% CO 2 .

[0141] To study the effect of the two scaffold materials on cell growth, we seeded rat bone mesenchymal stem cells (bMSCs) on the scaffolds and examined their growth status.

[0142] 2. Immunofluorescence (DAPI) detection

[0143] Firstly, the density and growth distribution of c...

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Abstract

The invention discloses a bionic bone tissue engineering bracket as well as a preparation method and application thereof. The method comprises the following steps: firstly, performing carboxylation and dopamine surface modification on carbon nano tubes (CNTs) in turn; forming hydroxyapatite grains on the dopamine CNTs surfaces under the condition of simulated body fluid, thereby constructing biological mineralized CNTs; utilizing agarose to construct a main body bracket and utilizing an electrophoretic technique to move the mineralized CNTs in the bracket and form an array in parallel in sequence, thereby constructing the bionic bone tissue engineering bracket AG-o-CNTs. The bionic bone tissue engineering bracket AG-o-CNTs disclosed by the invention can effectively promote the growth of mesenchymal stem cells (bMSCs) and has wide application prospect at the aspect of bone tissue engineering bracket material or bone substitute material.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials. More specifically, it relates to a biomimetic bone tissue engineering scaffold based on agarose gel and ordered multi-walled carbon nanotubes and its preparation method and application. Background technique [0002] The importance of bone tissue and the harm caused by bone-related diseases have accelerated the development of bone tissue engineering. In this context, bone tissue engineering has become a new cutting-edge method for repairing damaged organs due to its great potential to treat bone-related injuries or diseases. Generally speaking, the research of bone tissue engineering consists of three elements: scaffolds, cells and growth factors. Among the above three, the research on stents is one of the more active fields at present. Bone tissue engineering requires that the scaffolds used are ideal bone substitutes and have properties that can induce bone cells to form minerali...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/08A61L27/28A61L27/32A61L27/38
CPCA61L27/08A61L27/20A61L27/28A61L27/32A61L27/3834A61L27/3847A61L2400/18A61L2430/02C08L5/12
Inventor 关燕清陈吾雅
Owner SOUTH CHINA NORMAL UNIVERSITY
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