Preparation method of sustained release drug-loaded micro-capsule with multi-layer core shell structure
A multi-layer core-shell structure, microcapsule technology, applied in microcapsules, biochemical equipment and methods, medical preparations of non-active ingredients, etc., can solve the problem of multi-layer core-shell structure sustained-release drug microcarriers There are few reports and other problems, to achieve the effects of low preparation cost, reduced administration times, and easy operation.
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Embodiment 1
[0024] The present embodiment prepares slow-release drug-loaded microcapsules according to the following steps:
[0025] (1) Add 0.2 g of sodium carboxymethylcellulose (CMC) to 10 mL of 0.02 g / mL sodium alginate (Alg) solution, stir until dissolved, then add 0.3 g of bovine serum albumin and stir evenly, obtain the inner aqueous phase;
[0026] (2) Prepare 0.1g / mL CaCl 2 Aqueous solution and 0.05g / mL NaCl aqueous solution, the two solutions are mixed at a volume ratio of 1:1 to obtain an external aqueous phase;
[0027] (3) Use a disposable syringe to extract 2 mL of the inner water phase prepared in step (1), and inject it into the outer water phase (20 mL) in step (2) through an orifice at a constant speed at a rate of 450 μL / min to maintain a constant speed. During the injection process, the external aqueous phase was kept flowing at a low speed of 300 rpm under the action of a magnetic stirrer. After the injection was completed, solidify at room temperature for 15 minut...
Embodiment 2
[0031] The present embodiment prepares slow-release drug-loaded microcapsules according to the following steps:
[0032] (1) Add 0.3 g of sodium hyaluronate (HA) to 10 mL of 0.04 g / mL sodium alginate (Alg) solution, stir until dissolved, then add 0.4 g of insulin and stir evenly to obtain the inner aqueous phase;
[0033] (2) Prepare 0.13g / mL CaCl 2 Aqueous solution and 0.07g / mL NaCl aqueous solution, the two solutions are mixed at a volume ratio of 1:3 to obtain an external aqueous phase;
[0034] (3) Use a disposable syringe to extract 2 mL of the inner water phase prepared in step (1), and inject it into the outer water phase (20 mL) in step (2) through an orifice at a constant speed at a rate of 500 μL / min. The inner and outer water phases are kept flowing at a low speed of 330rpm under the action of a magnetic stirrer. After the injection is completed, cure at room temperature for 30 minutes, and collect the calcium alginate core embedded with HA (denoted as CA / HA core)...
Embodiment 3
[0039] The present embodiment prepares slow-release drug-loaded microcapsules according to the following steps:
[0040] (1) Add 0.2 g of cellulose sodium sulfate (CS) to 10 mL of 0.03 g / mL sodium alginate (Alg) solution, stir until dissolved, then add 0.4 g of bovine serum albumin and stir evenly to obtain the inner water box;
[0041] (2) Prepare 0.12g / mL CaCl 2 aqueous solution and 0.06g / mL NaCl aqueous solution, take the two solutions and mix them at a volume ratio of 1:5 to obtain the outer aqueous phase;
[0042] (3) Use a disposable syringe to extract 2 mL of the inner water phase prepared in step (1), and inject it into the outer water phase (20 mL) in step (2) through an orifice at a constant speed at a constant rate of 600 μL / min. During the injection process, the external aqueous phase was kept flowing at a low speed of 350 rpm under the action of a magnetic stirrer. After the injection was completed, the reaction was cured at room temperature for 45 minutes, and...
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