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Nanoparticle for macrophage targeting drug delivery and preparation method thereof

A technology of nanoparticles and macrophages, which is applied in the field of nano drug carriers, can solve the problems of easy decomposition and poor stability, and achieve the effects of increasing curative effect, high yield, and simple and fast preparation process

Inactive Publication Date: 2017-11-10
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Curcumin (CUR) is an active substance extracted from the rhizomes of turmeric, turmeric and other plants in the ginger family. It is relatively safe for humans, but it is easy to decompose in a neutral and alkaline environment and has poor stability. Due to these defects, it has not been widely used in clinical practice

Method used

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  • Nanoparticle for macrophage targeting drug delivery and preparation method thereof
  • Nanoparticle for macrophage targeting drug delivery and preparation method thereof
  • Nanoparticle for macrophage targeting drug delivery and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1 is the preparation method of the loaded CUR nanoparticle of chondroitin sulfate modification, comprises the following steps:

[0036] (1) Weigh 100 mg of PLGA and 6 mg of CUR and dissolve them in 2 mL of dichloromethane and methanol solution;

[0037] (2) Add the above organic solution dropwise into 4 mL of 5% polyvinyl alcohol solution;

[0038] (3) Immediately put the above solution into an ice bath, and perform ultrasonication with an amplitude of 50%, 10 s each time, 1 s interval, and a total ultrasonic time of 1 min;

[0039] (4) After the ultrasound is completed, quickly pour the above emulsion into 60 mL of 0.05% polyvinyl alcohol solution;

[0040] (5) At room temperature, volatilize the organic solvent in the above emulsion;

[0041] (7) The above-mentioned nanoparticles were collected by centrifugation and washed three times with deionized water at a centrifugation rate of 12000 rpm and a centrifugation time of 20 min;

[0042] (8) React the pr...

Embodiment 2

[0047] Embodiment 2 is the preparation method of the loaded CUR nanoparticle of carboxymethyl cellulose modification, comprises the following steps:

[0048] (1) Weigh 100 mg of PLGA and 6 mg of CUR and dissolve them in 2 mL of dichloromethane and methanol solution;

[0049] (2) Add the above organic solution dropwise into 4 mL of 5% polyvinyl alcohol solution;

[0050] (3) Immediately put the above solution into an ice bath, and perform ultrasonication with an amplitude of 50%, 10 s each time, 1 s interval, and a total ultrasonic time of 1 min;

[0051] (4) After the ultrasound is completed, quickly pour the above emulsion into 60 mL of 0.05% polyvinyl alcohol solution;

[0052] (5) At room temperature, volatilize the organic solvent in the above emulsion;

[0053] (7) The above-mentioned nanoparticles were collected by centrifugation and washed three times with deionized water at a centrifugation rate of 12000 rpm and a centrifugation time of 20 min;

[0054] (8) React th...

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Abstract

The invention discloses a nanoparticle for macrophage targeting drug delivery and a preparation method thereof. First, curcumin-carrying nanoparticles are prepared by oil-in-water solvent evaporation method, the surface of the curcumin-carrying nanoparticles is modified with chondroitin sulfate, the particle size of the nanoparticles is 281.5 + / -3.51nm, and the narrow particle size distribution and the higher drug loading amount can be rezlized. The chondroitin sulfate is introduced into the surface of the nanoparticles, and by scanning electron microscope observation, the nanoparticles are round or oval, and the size is more uniform. Compared with the nanoparticles with the surface modified with carboxymethyl cellulose, the chondroitin-sulfate-modified nanoparticles significantly increase the phagocytosis efficiency and anti-inflammatory effect, and the therapeutic efficiency of colitis can be effectively enhanced.

Description

technical field [0001] The invention belongs to the technical field of nano drug carriers, and in particular relates to a preparation method, characterization and application of drug-loaded porous nanoparticles. Background technique [0002] Ulcerative colitis (UC) is the most important type of inflammatory bowel disease. The main clinical symptoms are diarrhea, blood in the stool, weight loss, abdominal pain, fatigue and fever. According to data, the incidence of ulcerative colitis is relatively high in European and American countries. Taking the United States as an example, the incidence rate is as high as 2‰. Symptoms of UC usually get worse over time. Traditional treatment drugs mainly include aminosalicylic acid drugs (such as 5-aminosalicylic acid), immunosuppressant drugs (such as methotrexate) and glucocorticoid drugs (such as budesonide). [0003] In recent years, micro- and nano-particles based on polylactic-co-glycolic acid (PLGA) have been widely studied, which...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/61A61K31/12A61P1/00A61P1/04
CPCA61K31/12
Inventor 肖波马莉君汪敏
Owner SOUTHWEST UNIVERSITY
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