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Microorganism composite microbial agent, preparation method thereof and sewage treatment system

A technology of composite bacterial agent and microorganism, which is applied in the sewage treatment system, microbial composite bacterial agent and its preparation, and the field of domestic sewage degradation. It can solve the problems of long construction period, high operating cost and huge investment, and achieve small investment and high operation efficiency. Simple, quick results

Pending Publication Date: 2017-11-17
安徽瑞驰兰德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such a huge investment, long construction period, high operating costs, difficult to achieve

Method used

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  • Microorganism composite microbial agent, preparation method thereof and sewage treatment system
  • Microorganism composite microbial agent, preparation method thereof and sewage treatment system
  • Microorganism composite microbial agent, preparation method thereof and sewage treatment system

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preparation example Construction

[0033] The preparation method of the microbial composite bacterial agent is to inoculate and culture Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis and Bacillus coliformis in sequence respectively, and then inoculate and mass-produce the cultured strains according to the formula quantity. Produced after cross-antagonism culture.

[0034] Its specific preparation method comprises the following steps:

[0035] A. Inoculate Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, and Bacillus colioids in solid medium respectively, and culture for 36-48 hours at a culture temperature of 25-32°C; the solid medium uses agar.

[0036] B. Inoculate the bacterial classification after step A culture in the shake flask liquid culture medium respectively, cultivate 36~48h, culture temperature is 28~30 ℃, shake flask speed is 50~70r / min; Wherein, the liquid culture medium The ingredients are: each L contains 15g of sucrose, 12g of glucose, K 2 HPO 4 ...

Embodiment 1

[0049] A microbial composite bacterial agent for treating domestic sewage, comprising various strains in the following percentages by weight:

[0050] Bacillus amyloliquefaciens 20%, Bacillus subtilis 30%, Bacillus licheniformis 20% and Bacillus coliformis 30%.

[0051] Its preparation method comprises the following steps:

[0052] A. Inoculate Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, and Bacillus colioids on solid medium respectively, and cultivate them for 36-48 hours at a temperature of 25°C; the solid medium is agar.

[0053] B. Inoculate the strains cultivated in step A into the shake flask liquid culture medium respectively, cultivate for 36-48h, the culture temperature is 28°C, and the shake flask speed is 60r / min; wherein, the composition of the liquid culture medium is: L contains 15g of sucrose, 12g of glucose, K 2 HPO 4 0.3g, CaCO 3 1.5g, NaCl 0.3g. The liquid medium of the four bacteria is the same.

[0054] C. Inoculate the st...

Embodiment 2

[0058] A microbial composite bacterial agent for treating domestic sewage, comprising various strains in the following percentages by weight:

[0059] Bacillus amyloliquefaciens 30%, Bacillus subtilis 35%, Bacillus licheniformis 15% and Bacillus coliformis 20%.

[0060] A microbial composite bacterial agent for treating domestic sewage, comprising various strains in the following percentages by weight:

[0061] Bacillus amyloliquefaciens 20%, Bacillus subtilis 30%, Bacillus licheniformis 20% and Bacillus coliformis 30%.

[0062] Its preparation method comprises the following steps:

[0063] A. Inoculate Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus licheniformis, and Bacillus colioids on solid medium respectively, and cultivate them for 36-48 hours at a temperature of 32°C; the solid medium is agar.

[0064] B. Inoculate the strains cultivated in step A into the shake flask liquid culture medium respectively, cultivate for 36-48h, the culture temperature is 30°C, a...

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Abstract

The invention discloses a microorganism composite microbial agent, a preparation method thereof and a sewage treatment system. The microorganism composite microbial agent comprises the following strains, by weight, of 20-35% of bacillus amyloliquefaciens, 25-40% of bacillus subtilis, 15-30% of bacillus licheniformis and 20-30% of bacillus mucilaginosus. According to the microorganism composite microbial agent, rural domestic sewage can be effectively treated, and various pollutants in the sewage can be removed; through reasonable design of the sewage treatment system, in a first-stage degradation pool, a mixed microbial agent is added; and aerator pipes are installed in the degradation pool of every stage, and stage-by-stage overflow and aeration are achieved, so that the sewage in the pool of every stage has a chance to make contact with the microbial agent and then to be sufficiently degraded and decomposed.

Description

technical field [0001] The invention belongs to the technical field of domestic sewage treatment, and in particular relates to a microbial compound bacterial agent, a preparation method thereof and a system for treating sewage, which are suitable for the degradation of domestic sewage with small volume, high concentration and discontinuous discharge. Background technique [0002] At present, the main problems of domestic sewage in rural areas of our country are large volume, wide area, multiple points, and dispersion. Domestic sewage is mixed with kitchen waste and feces. Generally, it is only simple sedimentation, and some are even discharged directly. The pathogenic bacteria, COD, ammonia nitrogen, total nitrogen, and total phosphorus in the sewage exceed the standard, failing to meet the discharge standard. According to the treatment method of municipal sewage, a pipe network is built to collect it, and a sewage treatment plant is built for unified treatment. Such a huge...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F7/00C12R1/125C12R1/07C12R1/10
CPCC12N1/20C02F3/34C02F7/00C02F2203/006C02F2301/08Y02W10/10
Inventor 李建斌芮文东
Owner 安徽瑞驰兰德生物科技有限公司
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