Kit for detecting procalcitonin in blood and method for detecting procalcitonin

A detection kit and procalcitonin technology, which is applied in the biological field, can solve the problems of widening the detection range of sensitivity and long time to produce results, etc., and achieve the effect of solving long reaction time, reducing interference and improving sensitivity

Pending Publication Date: 2017-11-21
SUZHOU SYM BIO LIFESCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In order to solve the above-mentioned problems in the prior art, the present invention proposes a test kit for detecting procalcitonin in blood and a detection method for procalc

Method used

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  • Kit for detecting procalcitonin in blood and method for detecting procalcitonin
  • Kit for detecting procalcitonin in blood and method for detecting procalcitonin
  • Kit for detecting procalcitonin in blood and method for detecting procalcitonin

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Embodiment 1

[0053] The present embodiment provides a method for detecting procalcitonin in blood, comprising the steps of:

[0054] 1) Coat magnetic beads with streptavidin: Dilute the magnetic beads with 0.1M PBS buffer to 10 mg / mL; add according to the mass ratio of magnetic beads:streptavidin=1:8-1:12 Streptavidin, react at 37°C for 16-24 hours; after magnetic separation and washing, dilute into the blocking solution of Tris-Hcl system, the blocking solution contains 1% BSA, 0.1% casein, 3% sucrose, block at 37°C for 16 -24 hours, after magnetic separation and washing, dilute to 10 mg / mL with the above blocking solution and store to prepare streptavidin-coated magnetic beads;

[0055] 2) Label the first monoclonal antibody to procalcitonin with biotin: dialyze the first monoclonal antibody to procalcitonin and dilute it to 1 mg / mL with 0.1M PBS buffer, and the antibody by mass ratio: biotin = 1 : Add biotin at a ratio of 3-1:8, react at 25°C for 4 hours for labeling, dialyze with 0.1M...

Embodiment 2

[0062] This embodiment provides a kit for the detection method, including a reagent strip consisting of several wells arranged side by side, the reagent contained in the reagent strip is magnetic beads-SA-biotin-PCT monoclonal antibody solvent , Acridine ester-PCT monoclonal antibody solvent. The kit also includes sample diluent, calibrator, magnetic bead washing solution and pre-excitation solution. The corresponding reagents are sealed and stored in the corresponding holes on the reagent strips using heat-sealing film technology, which effectively prevents the reagents from crossing holes and solves the transportation problem.

[0063] In the test kit for the detection method of procalcitonin in this embodiment, a magnetic rod cover for supporting the use with a special upward-suction magnetic bead transfer gun is provided in a hole on the reagent strip. When the kit is used for the detection of procalcitonin, the up-suction magnetic bead transfer gun is used to transfer th...

Embodiment 3

[0067] This embodiment provides a method for using the detection kit. During detection, the reagent strip 4 is placed in the incubation chamber 3, the incubation chamber 3 is set on the conveyor belt 2, and the conveyor belt 2 is connected to Horizontal displacement motor 1, the end of described conveyer belt 2 is provided with analysis reading module 13; And adopts the up-absorbing type magnetic bead transfer gun that can move up and down relative to magnetic bar cover 5 to realize taking magnetic bar cover, absorbing magnetic bar cover on described reagent strip. Beads, magnetic bead elution, magnetic bead mixing, and magnetic rod cover removal.

[0068] Please continue to see figure 2 As shown, the magnetic rod cover 5 of the No. 0 position hole is extracted by an up-suction magnetic bead transfer gun, and the entire detection is carried out from right to left in a conjoined reagent strip. The sample is added to the No. 2 position hole, and the magnetic rod cover 5 5. Lif...

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Abstract

The invention discloses a kit for detecting procalcitonin in the blood and a method for detecting the procalcitonin. The method includes the steps of 1), coating magnetic beads with streptavidin; 2), marking a first monoclonal antibody of the procalcitonin with biotin; 3), combining the magnetic beads coated with the streptavidin with the first monoclonal antibody of the procalcitonin, marked with the biotin for reaction to obtain a magnetic bead-SA-biotin-PCT monoclonal antibody solvent; 4), marking acridinium ester with a second monoclonal antibody of the procalcitonin to obtain an acridinium ester-PCT monoclonal antibody solvent; 5), allowing the procalcitonin in a sample or a calibration product to react with the acridinium ester-PCT monoclonal antibody solvent and the magnetic bead-SA-biotin-PCT monoclonal antibody solvent; 6), after cleaning, calculating content of the procalcitonin in the sample. By the arrangement, the problem that coming out of results takes a long time is solved, the kit and the method can be applied to a peripheral whole blood sample, and detection range is widened without reducing flexibility.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting procalcitonin in blood and a detection method for procalcitonin. Background technique [0002] Procalcitonin (PCT) is a calcitonin propeptide substance without hormonal activity. Calcitonin is only produced when the C cells of the thyroid are stimulated by hormones. Procalcitonin can be produced by different types of cells in many organs. Secreted in response to pro-inflammatory stimuli, especially those caused by bacteria. [0003] Procalcitonin is an important marker that can specifically distinguish between bacterial infection and inflammatory response caused by other reasons. When severe bacterial, fungal, parasitic infection, sepsis and multiple organ failure level rises. Clinical data show that when the PCT concentration is >0.1ng / mL, it indicates that there is a clinically relevant bacterial infection, which needs to be treated with antibiotics; when t...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 张祥洪胡星海
Owner SUZHOU SYM BIO LIFESCI CO LTD
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