Microecological preparation with viable bacteria, preparation method and application thereof

A micro-ecological preparation, live bacteria technology, applied in the application, animal feed, additional food elements, etc., can solve the problems of not much improvement in thermal stability, poor tolerance, etc., achieve good biocompatibility, improve thermal stability The effect of stability and strong stability

Active Publication Date: 2017-11-24
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most live bacterial probiotics on the market can better solve the problems of storage and transportation, poor tolerance to gastric acid or bile, but there is not much improvement in thermal stability

Method used

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  • Microecological preparation with viable bacteria, preparation method and application thereof
  • Microecological preparation with viable bacteria, preparation method and application thereof
  • Microecological preparation with viable bacteria, preparation method and application thereof

Examples

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preparation example Construction

[0044] The present invention provides a kind of preparation method of live bacteria probiotics described in above-mentioned technical scheme, comprises the following steps:

[0045] (1) Adsorb the microorganisms in the porous adsorption material;

[0046] (2) Coat the surface of the porous adsorbent material obtained in step (1) with polysaccharide, vacuum treatment for 15 to 30 minutes in a wet state, dry at 40 to 60° C. for 8 to 15 minutes after the vacuum treatment, and then vacuum dry for 15 to 25 minutes , to get the core material;

[0047] (3) coating the surface of the core material obtained in the step (2) with a hydrophobic material to form a hydrophobic layer to obtain a hydrophobic layer coated core material;

[0048] (4) Coat the wall material with the hydrophobic layer obtained in the step (3) and coat the surface of the core material to form an outer wall layer, and dry to obtain a live bacterial microecological preparation.

[0049] In the present invention, t...

Embodiment 1

[0067] Take 10g of the quartz porous material and pulverize it to 80 mesh, and set it aside. Take 100mL, the number of live bacteria is 1×10 8 The CFU / g lactic acid bacteria suspension was centrifuged at 3000rpm and 4°C for 10min, the supernatant was removed, the precipitate was mixed with the pulverized quartz porous material, and vacuum treated at 0.06MPa and 45°C for 20min to obtain adsorption Quartz porous material with lactic acid bacteria.

[0068] Take 60 g of the porous quartz material with lactic acid bacteria adsorbed thereon, and suspend it in the fluidized bed. Take 3.2g gum arabic and 6.4g maltodextrin to prepare 48mL polysaccharide aqueous solution, and coat the quartz porous material with lactic acid bacteria adsorbed at a spray speed of 1.2mL / min; The above materials in wet state were vacuum treated for 20min under the conditions of 0.06MPa and 45°C. After vacuum treatment, dry at 45°C and normal pressure for 10 minutes, then transfer to a vacuum oven and dr...

Embodiment 2

[0074] Take 4g of sepiolite and crush it to 80 mesh, set aside. Take 100mL, the number of live bacteria is 1×10 9 Mix the CFU / g Enterococcus faecalis bacteria suspension with the pulverized sepiolite above, vacuum treatment at 0.06MPa, 45°C for 5min, centrifuge at 3000rpm, 4°C for 10min after vacuum treatment, and remove the supernatant , that is, sepiolite adsorbed with Enterococcus faecalis.

[0075] Take 80 g of the porous adsorption material adsorbed with Enterococcus faecalis and suspend it in the fluidized bed. Take 3.75g of gum arabic and 3.75g of maltodextrin to prepare 62.5mL of polysaccharide aqueous solution, and coat the porous adsorption material adsorbed with Enterococcus faecalis at a spray speed of 1mL / min; after the coating is completed, add 2g of talcum powder, Keep the flowing state for 5 minutes; vacuum treat the above-mentioned material in the wet state for 15 minutes under the conditions of 0.04MPa and 40°C. After vacuum treatment, dry at 40°C and norm...

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Abstract

The invention provides a microecological preparation with viable bacteria and relates to the technical field of the microecological preparation. The microecological preparation comprises a core material, a hydrophobic layer and an outer wall layer, wherein the hydrophobic layer and the outer wall layer are coated on the core material in sequence; the core material consists of a porous adsorbing material adsorbed with microorganisms and a polysaccharide layer coated on the surface of the porous adsorbing material; and the parts between pores of the porous adsorbing material and the polysaccharide layer are hollow. The microecological preparation provided by the invention has the advantages that the viable-bacteria microorganisms are put into the hollow pores of the core material, and then with the hydrophobic layer and the outer wall layer coated on the core material in sequence, hot steam and the like are not easy to enter the core material and contact with the microorganisms through outer-layer coatings, and the influences of external bad environments with high temperature, high humidity and high pressure and the like on the activity of the microorganisms can be effectively avoided, so that the stability of the microecological preparation with the viable bacteria is improved and the loss of the microecological preparation with the viable-bacteria microorganisms in the process of after processing is reduced.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a living microecological preparation and a preparation method and application thereof. Background technique [0002] Microecological preparations refer to preparations made by special processes using the principles of microecology, using probiotics or probiotic growth-promoting substances that are beneficial and harmless to the host. At present, probiotics have been used in various fields such as feed, agriculture, medicine and health care, and food. The active ingredients of the probiotics can be live bacteria, dead bacteria and their metabolites. The difference between the probiotics of live bacteria and the probiotics of dead bacteria or microbial metabolites is that the probiotics of live bacteria are Efficacy was significantly correlated with the number of viable bacteria in the probiotics. [0003] Most of the existing feed microecological preparations are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/163A23K20/28A23K20/147A23K20/105A23K20/22A23K40/30
Inventor 董颖超薛敏秦玉昌吕学泽李军国段海涛牛力斌
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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