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Closed biological membrane type culture method for industrial production

A cultivation method and biofilm technology, which is applied in the field of closed biofilm culture for industrial production of microalgae, can solve problems such as microalgae biomass pollution, achieve cell detachment and biofilm shedding, reduce dehydration costs, and reduce usage Effect

Inactive Publication Date: 2017-11-24
李彤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention provides a closed biofilm culture method for industrial production of microalgae, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the problem that the microalgae biomass in the existing microalgae culture method is easily polluted by miscellaneous bacteria, etc. the problem of contamination

Method used

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  • Closed biological membrane type culture method for industrial production

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Effect test

Embodiment 1

[0023] Embodiment 1: as attached figure 1 As shown, the closed biofilm culture method for industrialized production of microalgae is carried out as follows: one surface of the microporous film 1 is used as the microalgae cell inoculation surface 2, and the microalgae cell inoculation surface 2 is opposite to the microalgae cell inoculation surface. The other surface of the porous film 1 is used as the supply surface 3, and the microalgae cells 5 are inoculated on the microalgae cell inoculation surface 2. The microalgae cells 5 cannot pass through the micropores of the microporous film 1 to reach the supply surface 3. After inoculation, the microalgae cells The algae cell inoculation surface 2 is covered with a transparent film 4, and the gaseous phase and culture solution containing carbon dioxide are supplied to the microalgae cells 5 from the supply surface 3, and the carbon dioxide and the culture solution can pass through the micropores of the microporous film 1 to reach ...

Embodiment 2

[0028] Embodiment 2: as the optimization of above-mentioned embodiment, microporous film 1 is the filter material that aperture is less than microalgae cell 5, and filter material is filter paper or filter membrane or fiber fabric; And / or, transparent film 4 is transparent nontoxic film Material, the transparent film 4 is a semi-permeable film or a non-porous transparent film, and the aperture of the semi-permeable film is less than or equal to the aperture of the microporous film 1.

[0029] The filter material can adopt the existing cheap material, thereby reducing the production cost.

Embodiment 3

[0030] Embodiment 3: As an optimization of the above embodiments, the surfaces of the microporous film 1 and the transparent film 4 are flat or curved.

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Abstract

The invention relates to the technical field of microalgae culture methods and particularly relates to a closed biological membrane type culture method for industrial production. The culture method comprises the following step: with one surface of a microporous membrane as a microalgae cell inoculation surface as well as the other surface, opposite to the microalgae cell inoculation surface, of the microporous membrane as a feeding surface, inoculating microalgae cells to the microalgae cell inoculation surface. According to the closed biological membrane type culture method for the industrial production, microalgae biomass and a nutrient solution are separated through the microporous membrane, so that the problems of cell detachment and biological membrane falling are thoroughly solved; by virtue of a separation effect of the microporous membrane, pollutants such as infectious microbes in the nutrient solution cannot enter the microalgae biomass; by virtue of a coverage isolation effect of a transparent membrane, the pollution caused by pollutants such as external infectious microbes to the microalgae biomass is avoided, so that the impurity of the microalgae biomass is increased (reaching above 99%), and the quality of the microalgae biomass is improved.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation methods, and relates to a closed biofilm cultivation method for industrial production of microalgae. Background technique [0002] Microalgae refer to eukaryotic or prokaryotic microorganisms that can perform photosynthesis. Some microalgae have high protein content and can be used as food, aquatic bait or livestock feed (such as spirulina); some microalgae can synthesize a large amount of secondary metabolites under specific conditions, such as oil, pigment, polysaccharide, etc. Astaxanthin produced by Haematococcus), these products can be used in functional food, food additives, pharmaceuticals, bioenergy and other fields, and have considerable economic value. In addition, extracting microalgae oil from biomass and transforming it into biodiesel is considered to be one of the most important ways to sequester carbon and reduce emissions. At present, on a global scale, microalgae ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12M1/00C12R1/89
CPCC12M25/02C12N1/12
Inventor 李彤
Owner 李彤
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