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Cockroach bait agent containing cockroach virus, preparation method and applications thereof

A technology of cockroaches and viruses, applied in the field of biopesticides, can solve the problem of unsatisfactory luring and feeding effects, and achieve the effects of human and animal safety, strong specificity, and no pollution to the environment

Inactive Publication Date: 2017-12-05
WUHAN HANTE ENVIRONMENT SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of attracting food is not very ideal

Method used

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  • Cockroach bait agent containing cockroach virus, preparation method and applications thereof
  • Cockroach bait agent containing cockroach virus, preparation method and applications thereof
  • Cockroach bait agent containing cockroach virus, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-15

[0036] A cockroach-killing bait containing cockroach virus, comprising the following components:

[0037]

[0038]

[0039] The extraction method of described cockroach pheromone is:

[0040] Cockroach pheromone extraction method: Take a soaking bucket, weigh and add 1 kg of fresh cockroach feces, add 3 kg of absolute ethanol, homogenize with a high-shear emulsifier, and soak for 24 hours. Then the slurry is filtered with a manual filter press to obtain 2 to 2.5 kg of yellow transparent filtrate. The filtrate was added to a rotary evaporator, and the alcohol solvent was evaporated at 20-25°C under a vacuum of 76 mm Hg, and finally about 10 ml of a yellow-brown liquid substance with a special foul smell was obtained. After testing, it contains cockroach aggregation pheromone, sex pheromone, and other metabolites.

[0041] The cockroach virus was purchased from Baile Biotechnology Co., Ltd.

Embodiment 16

[0043] A preparation method of a cockroach-killing bait containing cockroach virus, comprising the following preparation steps:

[0044] In this embodiment, the formula shown in Example 6 is taken as an example for illustration.

[0045] ① Dissolve sucrose, maltose syrup, sodium benzoate and calcium lactate in hot water and set aside;

[0046] ② Mix glycerin and sodium carboxymethyl cellulose;

[0047] ③ Pour the mixed solution of ① into ②, and stir homogeneously in a vacuum emulsification mixer for about 30 minutes;

[0048] ④ Pour the corn flour and calcium carbonate into the vacuum emulsification mixer respectively, and continue to homogeneously stir for about 30 minutes.

[0049] ⑤Through the cooling water, cool the materials in the pot to about 25°C, mix the cockroach virus solution, cockroach pheromone, and fluorescein sodium evenly into the emulsification pot, vacuumize, and stir for 30 minutes.

Embodiment 17

[0052] Application of a cockroach-killing bait containing cockroach virus in the preparation of cockroach insecticide:

[0053]1. Test insects German cockroaches (Blattella germanica), 10-15 days old adults, half female and half male.

[0054] 2. Test method

[0055] 1) Put 30 Blattella germanica into the square box device with the door closed, plug the worm hole tightly, and after the tested insects resume normal activities, place 10 grams of bait to be tested in one corner of the box, and place Blattella germanica feed on the opposite corner , and placed in the center of the petri dish filled with water-soaked cotton balls. Observe the death of test insects every day, take out and count the dead insects, and observe continuously until the 12th day after feeding. The test should be repeated three times or more, and the normal feed is used as a blank control. The experimental device should be cleaned after each experiment. The experimental results are shown in Table 1, Table...

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Abstract

The present invention discloses a cockroach bait agent containing cockroach virus, a preparation method and applications thereof, wherein the main components of the dosage form are cockroach virus and cockroach pheromone, the cockroach virus inclusion body content is 6000-60000 / g, and the cockroach bait agent further comprises sucrose, corn powder, malt syrup, glycerin, sodium benzoate, calcium carbonate, sodium acetate, calcium lactate, fluorescein sodium, sodium carboxymethyl cellulose and water according to a certain ratio. According to the present invention, the cockroach bait has characteristics of no harm to environment, organisms, animals and human body, no environmental pollution and no drug resistance, can effectively control the occurrence and the harm of cockroach, is suitable for families, hotels, offices, kindergartens, schools, hospitals, food factories, aircrafts and other special occasions, and can ensure the disappearance of cockroaches for one year.

Description

technical field [0001] The invention relates to biological insecticides, more specifically to a cockroach-killing bait containing cockroach virus, a preparation method and application. Background technique [0002] Cockroaches are worldwide important sanitary pests, polluting food, spreading diseases, and seriously threatening human health. There are 13 species of 4 families, 6 genera and 13 species of indoor cockroaches known in my country. Due to convenient transportation and suitable indoor temperature and humidity, cockroaches have basically covered places except the Arctic and Antarctic. They reproduce quickly, have strong resistance, and strong concealment, making it difficult to eradicate cockroaches. [0003] At present, cockroach control is mainly based on chemical control. The main dosage forms include suspension concentrate, emulsifiable concentrate, water-emulsion and bait. Suspended concentrate, emulsifiable concentrate and water-emulsion are mainly retained sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N61/00A01P7/04A01P19/00
CPCA01N63/00A01N61/00A01N2300/00
Inventor 王柏海胡家鑫张宁尹宜农李胜林刘取
Owner WUHAN HANTE ENVIRONMENT SCI & TECH
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