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Preparation method and application of composite photo-thermal antimicrobial agent of antimicrobial peptide modified gold nanorod

A technology of gold nanorods and antibacterial peptides, which is applied in the field of photothermal antibacterial preparation, can solve the problems of poor absorption capacity, digestion and degradation of antibacterial peptides, poor bacterial selectivity, etc., and achieve good antibacterial effect

Active Publication Date: 2017-12-08
兰州博林化工科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the photothermal materials under research mainly include noble metal nanoparticles (advantages: strong NIR absorption, easy to combine with biomolecules; disadvantages: poor bacterial selectivity), carbon nanomaterials (advantages: large photothermal conversion area; disadvantages: near Poor absorption in the infrared region), metal nonmetal compounds (advantages: low cost, easy synthesis; disadvantages: low photothermal conversion efficiency), polymer nanomaterials (advantages: easy to form antibacterial coatings, thin films; disadvantages: photothermal conversion Low efficiency, poor biocompatibility), inorganic biocomposite materials (advantage: identification of bacteria; disadvantage: no bactericidal performance by itself), etc.
Antimicrobial peptides themselves have good antibacterial effects, but single antimicrobial peptides are easily digested and degraded by enzymes in the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of AuNRs: First, add 0.25mL HAuCl4 solution to 7.5mL CTAB solution, stir evenly, then dropwise add 0.6mL NaBH4 solution prepared in ice water, shake and mix well, and place in a 30°C water bath for more than 3h. Get the gold seed. Take 0.05mL gold seed solution, add it to the growth solution containing 23.75mL CTAB solution, 1mL HAuCl4 solution, 0.15mL AgNO3 solution and 0.16mLAA solution, stir evenly, and place it in a 30°C water bath for more than 3h to obtain the AuNRs solution. AuNRs were purified by centrifugation (12000rmp, 10min) twice before use.

[0029] (2) Preparation of AMPs solution: Dissolve 1 mg of antimicrobial peptide powder with amino acid sequence RIWVIERRC in 1 mL of hexafluoroisopropanol solution, shake and dissolve at room temperature overnight to obtain hexafluoroisopropanol-dispersed peptide solution. Take 0.012mL of hexafluoroisopropanol-dispersed polypeptide solution in a centrifuge tube, seal the mouth of the centrifuge tube w...

Embodiment 2

[0033] Same as Example 1, only the volume of the hexafluoroisopropanol-dispersed antimicrobial peptide used for drying in step (2) of Example 1 was changed to 0.02mL and 0.06mL. And the final molar ratio of AuNRs solution to AMPs solution is 20:1, 10:1. The bactericidal efficiency of the prepared Au@AMPs is shown in Table 1. The results showed that as the molar ratio of AuNRs to AMPs decreased, that is, the amount of modified antimicrobial peptides increased, the bactericidal efficiency of the prepared Au@AMPs increased. This shows that antibacterial provides a good bactericidal effect in it.

[0034] Table 1 The impact of different antimicrobial peptide modification amounts on the bactericidal efficiency of the prepared material

[0035] Molar ratio of AuNRs to AMPs

Embodiment 3

[0037] Same as Example 1, only change the power of the near-infrared light used in step (4) of Example 1 to 0.6W, 0.8W, 1W to study its photothermal antibacterial effect by changing the irradiation power. The bactericidal efficiency obtained is shown in Table 2. The results showed that the photothermal sterilization efficiency of Au@AMPs gradually increased with the increase of power.

[0038] The influence of table 2 different irradiation powers on the bactericidal efficiency of prepared material

[0039] Irradiation power (W)

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Abstract

The invention discloses a preparation method and an application of a composite photo-thermal antimicrobial agent of an antimicrobial peptide modified gold nanorod, and belongs to the technical field of material preparation. Specifically, the invention provides the method that the gold nanorod is modified by virtue of antimicrobial polypeptide and synergistic and efficient sterilization is implemented on the basis of an antimicrobial effect of the antimicrobial peptide and a photo-thermal effect of the antimicrobial peptide generated under near-infrared irradiation, and the method mainly comprises the following steps: (1) preparing a gold seed; (2) preparing the gold nanorod; (3) preparing an antimicrobial polypeptide solution; (4) preparing a composite photo-thermal agent of the antimicrobial peptide modified gold nanorod; and (5) implementing photo-thermal bacterium resistance. According to the technology, the antimicrobial polypeptide is modified on the gold nanorod which has a strong NIR absorption effect and is applied to the photo-thermal bacterium resistance. A better synergistic and efficient antimicrobial effect can be achieved with relatively low energy input on the basis of the relatively good antimicrobial effect of the antimicrobial peptide and the photo-thermal effect of the antimicrobial peptide generated under the near-infrared irradiation.

Description

technical field [0001] The present invention relates to a preparation method and application of a photothermal antibacterial agent, in particular to the use of antibacterial polypeptides to modify gold nanorods, through the antibacterial effect of the antibacterial peptide itself and the photothermal effect produced under near-infrared light irradiation, synergistically efficient Sterilization belongs to the technical field of material preparation. Background technique [0002] At present, the problem of overuse of antibiotics is becoming more and more serious around the world, especially in developing countries. According to statistics, the global consumption of antibiotics in 2013 was about 330,000 tons; the consumption of antibiotics in my country reached 162,000 tons, accounting for about half of the global consumption. Due to the large-dose use and abuse of antibiotics, many new multidrug-resistant (MDR) "super bacteria" have emerged in the past 20 years, such as methi...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K38/08A61P31/04B22F9/24B22F1/00B82Y40/00B82Y30/00
CPCA61K38/08A61K41/0052B82Y30/00B82Y40/00B22F9/24B22F1/054B22F1/145A61K2300/00
Inventor 刘磊张杰冯永海陈清玉董明东
Owner 兰州博林化工科技有限公司
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