The invention relates to a preparation method of biocompatible photo-thermal response self-healing conductive hydrogel, which includes: preparing solution from N,N-dimethyl acrylamide monomer, potassium persulfate serving as initiator and N,N,N',N'-tetramethylethylenediamine serving as accelerator; then soaking graphene hydrogel into the monomer solution for solution displacement and standing; and finally, initiating polymerization reaction at the room temperature, so that the biocompatible photo-thermal response self-healing conductive hydrogel is prepared. The preparation method is simple in process, mild in reaction condition, short in reaction time and suitable for industrial production. The hydrogel uses the graphene as a three-dimensional network framework, the graphene is high in reduction degree, chemical stability, electric conductivity and mechanical strength and good in photothermal conversion effect, and the hydrogel shows good self-healing performance within the body temperature range and under near-infrared irradiation.